ABSTRACT
<p><b>OBJECTIVE</b>To observe the clinical efficacy and action mechanism of acupuncture for ovulatory disorder infertility of ovarian induction period with clomiphene citrate (CC).</p><p><b>METHODS</b>Through retrospective analysis, 36 patients with ovulatory disorder infertility were divided into CC group (16 cases) and acupuoture group (20 cases) by method. The patients in the CC group were treated with oral administration of CC (50 mg) for 5 days from the 5th day of menstruation; based on the treatment of CC, patients in the acupuncture group were treated with acupuncture; the group A of acupoint was mainly consisted of acupoints of the conception vessel and the three meridians and front- acupoints in abdomen; the group B of acupoint was mainly consisted of acupoints of the governor vessel and back- acupoints. The two groups of the acupoints were selected alternately, combined with the -guan points (Taichong (LR 3)、Hegu (LI 4)) and five- points. The needles were inserted with flying needling method, and the needles were retained for 30 min per treatment; acupuncture was given once every other day. All the patients were treated for 2 months, and treatment stopped if pregnancy occurred. The pregnancy rate, cycle ovulation rate, maximum diameter of follicle, endometrial thickness and adverse effects were compared between the two groups.</p><p><b>RESULTS</b>The pregnancy rate of the acupuncture group was 75.0% (15/20), which was superior to 37.5% (6/16) in the CC group (<0.05). The cycle ovulation rates of two groups were 70.4% (19/27, >0.05). The maximum diameter of follicle and the endometrium thickness in the acupuncture group were superior to those in the CC group (both <0.05). The incidence of adverse effects in the acupuncture group was 0% (0/20), which was lower than 18.8% (3/16) in the CC group (<0.05).</p><p><b>CONCLUSION</b> acupuncture combined with CC could improve the quality of follicle and the receptivity of endometrium in patients with ovulatory disorder infertility, so as to improve the pregnancy rate, which could be used as a safe and effective means to cooperate with modern assisted reproductive technology.</p>
ABSTRACT
The effect of age, follicular diameter and month of the breeding season (September to January) on the hCG induction of ovulation was evaluated using 123 Criollo mares. Age varied between two and 24 years and the animals were examined daily by rectal palpation and ultrasonography with a 5 MHz linear transducer. When ovarian follicles reached a diameter of 30 to 35 mm, ovulation was induced with an i.v. injection of 1000 IU (n = 39); 1500 IU (n = 41) or 2000 IU (n = 43) of hCG. The mares were bred the next day and examined daily until ovulation was detected. The percentage of mares ovulating before 24 h of hCG injection was 10.3%, 7.3% and 4.7%; until 48 h after injection 92.3%, 85.3% and 86.0% of the mares treated with 1000, 1500 and 2000 IU of hCG, respectively, ovulated. The month of the breeding season, age of the mares and follicular diameter had no influence on ovulatory response. The three hCG doses used in Criollo mares (P > 0.05) result in the induction of ovulation within 48 h after injection when a pre-ovulatory follicle with a 30 to 35 mm diameter was identified. A single dose of 1000 IU of hCG is efficient to induce ovulation in Criollo mares.
O efeito da idade, diâmetro folicular e mês da estação de monta (setembro a janeiro) na indução da ovulação com hCG foi avaliado em 123 éguas Crioulas. A idade das éguas variou entre dois e 24 anos e os animais foram examinados diariamente por palpação retal e ultrassonografia com transdutor linear de 5 MHz. Quando os folículos ovarianos atingiram diâmetro de 30 a 35 milímetros aplicou-se uma injeção intravenosa com 1000 UI (n = 39); 1500 UI (n = 41) ou 2000 UI (n = 43) de hCG. As éguas foram cobertas no dia seguinte e examinadas diariamente até a detecção da ovulação. O percentual de éguas que ovularam antes de 24 h da injeção de hCG foi de 10,3%, 7,3% e 4,7%, até 48h após a injeção foi de 92,3%, 85,3% e 86,0%, nos grupos com 1000, 1500 e 2000 UI de hCG, respectivamente. O mês da estação de monta, a idade das éguas ou o diâmetro folicular não influenciaram a resposta ovulatória. As três doses de hCG utilizadas em éguas Crioulas (P > 0,05) resultaram na indução da ovulação dentro de 48h após a aplicação, quando foi identificado um folículo pré-ovulatório de 30 a 35 mm de diâmetro. Uma única dose de 1000 UI de hCG é eficiente para induzir a ovulação em éguas Crioulas.