ABSTRACT
Resumen El objetivo del estudio fue determinar la digestibilidad aparente de la materia seca, proteína y carbohidratos de la dieta con ensilado biológico de harina de maíz en Cryphiops (C.) caementarius. Camarones adultos (4.50 a 7.20 cm de longitud total), se colectaron del río Pativilca (Lima, Perú) y en laboratorio se mantuvieron en recipientes individuales instalados dentro de seis acuarios (cuatro camarones por acuario). La dieta control fue sin ensilado y para la dieta experimental se reemplazó el 75% de la harina de maíz por el ensilado biológico de harina de maíz; ambas dietas tuvieron 1% de óxido crómico como marcador inerte. Los camarones fueron alimentados dos veces por día (40% a las 08:00 h y 60% a las 18:00 h) con el 6% del peso húmedo, durante 10 días. Para la colección de heces, los camarones fueron trasladados a un sistema de caja cribada (12 compartimentos) instalada dentro de un acuario. La digestibilidad aparente se determinó en base seca. Los camarones alimentados con la dieta con ensilado biológico de harina de maíz tuvieron altos coeficientes de digestibilidad aparente de carbohidratos (95.69%), proteína cruda (80.02%) y materia seca (76.19%), que aquellos alimentados con la dieta sin ensilado (p<0.05) que fue de 93.48, 78.21 y 74.36%, respectivamente. Los camarones adultos digieren con eficiencia los carbohidratos de la dieta con ensilado biológico de harina de maíz, en vez de la proteína de dicha dieta.
Abstract The aim of this study was to determine the apparent digestibility of dry matter, protein and carbohydrates of the diet with biological corn meal silage in Cryphiops (C.) caementarius. Adult prawn (4.50 to 7.20 cm total length) was collected from the Pativilca river (Lima, Peru) and in the laboratory each shrimp was kept in individual containers set up in six aquaria (four prawn per aquarium). The experimental diet was supplemented with corn meal biological silage and the control diet was without silage; both diets had 1% chromic oxide. Each diet had three replicates. Prawn was fed twice a day (40% at 08:00 h and 60% at 18:00 h) at 6% of wet weight for 10 days. For feces collection, the prawn was transferred to a system of sieved boxes (12 compartments) installed inside an aquarium. Apparent digestibility was determined on a dry basis. Prawn fed the diet with corn meal biological silage had higher apparent digestibility coefficient for carbohydrates (95.69%), crude protein (80.02%) and dry matter (76.19%) than those fed the diet without silage (p<0.05), which was 93.48, 78.21 and 74.36%, respectively. The adult prawn digested efficiently the carbohydrates of the corn meal biological silage diet, instead of the protein of this diet.
ABSTRACT
Resumen El objetivo fue determinar la preferencia termal de machos adultos de Cryphiops caementarius previamente aclimatados a diferentes temperaturas. Los camarones se colectaron del río Pativilca. En laboratorio, los camarones se aclimataron durante 30 días en nueve acuarios, cada uno con 10 recipientes circulares. En cada recipiente hubo un camarón (10 por acuario). Las temperaturas de aclimatación fueron de 19, 24 y 28 °C. El sistema de gradiente de temperatura (12 a 33 °C) fue una canaleta de PVC (3 m de longitud y 10 cm de profundidad) con 18 compartimentos. En un extremo de la canaleta se colocaron termorreguladores sumergibles (100W) y en el otro extremo, bolsas con hidrogel congeladas. La preferencia termal se determinó por el método agudo y las pruebas se realizaron entre las 08:00 y 14:00 h. Los camarones aclimatados a 24 °C se desplazaron a compartimentos cuyas temperaturas fueron más altas que la temperatura de aclimatación. Los camarones aclimatados a 24 °C se desplazaron a compartimentos cuyas temperaturas fueron alrededor de la temperatura de aclimatación. Los camarones aclimatados a 28 °C se desplazaron a compartimentos donde las temperaturas fueron más bajas que aquella de aclimatación. La preferencia termal de los camarones aclimatados a 19 °C fue de 22.7 °C, y la de los aclimatados a 24 °C fue de 24.8 °C, ambos sin diferencias significativas (p>0.05). En cambio, en los aclimatados a 28°C fue de 21.1 °C, que solo difirió (p<0.05) con los aclimatados a 19 °C. La preferencia termal final fue de 24.5 °C.
Abstract In this work, we establish the thermal preference of Cryphiops caementarius adult males, previously acclimated at different temperatures. Shrimp were collected from the Pativilca River. In the laboratory, shrimp were acclimated for 30 days in nine aquariums, each with 10 circular containers. In each container, there was a shrimp (10 per aquarium). Acclimation temperatures were 19, 24 and 28 °C. The gradient temperature system (12 to 33 °C) consisted for a PVC gutter (3 m long and 10 cm deep) with 18 compartments. Submersible thermoregulators (100W) were placed at one end of the gutter and the other end, frozen hydrogel bags. The thermal preference was determined by the acute method and the tests were performed between 08:00 and 14:00 h. Shrimp acclimated at 24 °C moved to compartments whose temperatures were higher than the acclimation temperature. Shrimp acclimated at 24 °C moved to compartments whose temperatures were around the acclimation temperature. Shrimp acclimated at 28 °C moved to compartments where temperatures were lower than acclimation. The thermal preference of acclimated shrimp at 19 °C was 22.7 °C, and those acclimated at 24 °C were 24.8 °C, both without significant differences (p>0.05). On the other hand, in acclimated ones at 28 °C it was 21.1 °C, which only differed (p<0.05) with those acclimated at 19 °C. The final thermal preference was 24.5 °C.
ABSTRACT
Ecdysis is a common phenomenon that happens throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii. It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process. The objective of the present study was to determine the amino acid profile released by M. rosenbergii during the ecdysis process that promotes cannibalism. To accomplish this, changes in amino acid levels (total amino acid (TAA) and free amino acid (FAA)) of tissue muscle, exoskeleton, and sample water of culture medium from the moulting (E-stage) and non-moulting (C-stage) prawns were analysed using high-performance liquid chromatography (HPLC). Comparison study revealed that among the TAA compounds, proline and sarcosine of tissues from moulting prawn were found at the highest levels. The level of FAA from water that contains moulting prawns (E-stage) was dominated by tryptophan and proline. Significant values obtained in the present study suggested that these amino acid compounds act as a chemical cue to promote cannibalism in M. rosenbergii during ecdysis. The knowledge of compositions and compounds that were released during the moulting process should be helpful for better understanding of the mechanism and chemical cues that play roles on triggering cannibalism, and also for future dietary manipulation to improve feeding efficiencies and feeding management, which indirectly impacts productivity and profitability.
ABSTRACT
Ecdysis is a common phenomenon that happens throughout the life phase of the giant freshwater prawn Macrobrachium rosenbergii. It is vital to better understand the correlation between cannibalism and biochemical compound that exists during the moulting process. The objective of the present study was to determine the amino acid profile released by M. rosenbergii during the ecdysis process that promotes cannibalism. To accomplish this, changes in amino acid levels (total amino acid (TAA) and free amino acid (FAA)) of tissue muscle, exoskeleton, and sample water of culture medium from the moulting (E-stage) and non-moulting (C-stage) prawns were analysed using high-performance liquid chromatography (HPLC). Comparison study revealed that among the TAA compounds, proline and sarcosine of tissues from moulting prawn were found at the highest levels. The level of FAA from water that contains moulting prawns (E-stage) was dominated by tryptophan and proline. Significant values obtained in the present study suggested that these amino acid compounds act as a chemical cue to promote cannibalism in M. rosenbergii during ecdysis. The knowledge of compositions and compounds that were released during the moulting process should be helpful for better understanding of the mechanism and chemical cues that play roles on triggering cannibalism, and also for future dietary manipulation to improve feeding efficiencies and feeding management, which indirectly impacts productivity and profitability.
Subject(s)
Animals , Amino Acids/chemistry , Aquaculture , Cannibalism , Chromatography, High Pressure Liquid , Feeding Behavior , Fresh Water , Malaysia , Molting , Palaemonidae/physiology , Proline/chemistry , Tryptophan/chemistryABSTRACT
Effects of estradiol-17β on the giant freshwater prawn were observed in case of the embryonic tolerance, growth, development, survival rate, yolk adsorption, eye appearance, and muscular compactness. The methods were designed in two steps; those were the tolerance and the growth. In the two hours after spawning, embryos were immersed in 10, 50, 100, or 150 µg/ml estradiol-17β solution for two days, and the controls were treated with 15% artificial seawater. The tolerance was monitored in a separate experiment; they were immersed in 10, 50, 100, or 150 µg/ml estradiol-17β solutions showed that the mortality rate at 150 µg/ml estradiol-17β was 31.66%. The growth represented by yolk adsorption, hatching rate, and eye appearance. The results showed that the eyes appeared earlier than those of the controls. The survival rates were investigated in other experiments. The results showed that the highest survival rate was 48.16%, observed in the 50 µg/ml estradiol-17β treatment. Therefore, estradiol might accelerate the growth as indicated by the number of days for eye appearance being shorter period than in the control treatment. The eyes of the embryos treated with 50, 100, and 150 µg/ml estradiol-17β appeared on day 10, whereas those in the control were observed on day 16. Hatching rate was tending to high in 150 µg/ml estradiol-17β but those were not significance with the control. Yolk adsorption was found in treated embryo rather than those of the controls. The pattern of yolk cluster distribution was differing from the control. The muscle tissue was observed on day 20 after the histological process. The results showed that the bundles muscle cells were more compactness and were larger, denser, and stronger with increasing concentrations of estradiol-17β than that the controls. Therefore, estradiol-17β should be applied to stimulate growth and might be introduced with the feed to the prawn industry and manufacturing
ABSTRACT
Macrobrachium amazonicum is a freshwater prawn endemic to South America with wide distribution in Brazilian Amazon rivers. In estuary and freshwater streams of the Pará State, they are captured with different types of traps locally know matapi. This study evaluated the efficiency of traps of different sizes (large, medium and small) and baits (babassu coconut and fish) for sampling this shrimp. Samplings were conducted with 24 traps with different treatments (trap size and bait). We captured 909 specimens. Higher mean catches were observed in traps baited with babassu coconut. Interactions between babassu coconut bait and medium matapi (BM-M), and fish bait and large matapi (FISH-L) were significant. Carapace length (CL) varied significantly between sites (F = 12.74, p < 0.01). The total maximum length was 13.65 cm. Medium traps baited with babassu coconut were the most successful in the tested combinations, however, there was a clear correlation between size trap and size of shrimp, for both body weight and carapace length.
Macrobrachium amazonicum é uma espécie de camarão endêmico da América do Sul e está amplamente distribuída nos rios da bacia amazônica brasileira. Nos estuários e cursos de água do estado do Pará são capturados com uso de armadilhas tradicionais conhecidos como 'matapis'. O presente estudo incidiu sobre as técnicas utilizadas pelos pescadores locais, no estado do Pará, além de avaliar a eficácia de diferentes tipos de armadilhas (com tamanhos grande, médio e pequeno) e iscas (de peixe ou farelo de babaçu). As amostras foram obtidas por 24 armadilhas sob diferentes tratamentos (tamanho da arte e isca). Foram capturados 909 indivíduos. As capturas médias nos locais foram maiores nas armadilhas com farelo de babaçu. As interações entre isca babaçu e matapis de tamanho médio (BM- M), e isca de peixe e grandes matapis (FISH- L) foram significativas. O comprimento da carapaça (CL) variou significativamente entre os locais (F = 12,74, p < 0,01). O comprimento máximo total registrado foi de 13,65 cm. As armadilhas de médio porte com isca de babaçu foram os mais bem sucedidos nas combinações testadas, no entanto, houve uma clara relação entre o tamanho da armadilha e o tamanho do camarão capturado, tanto em termos de peso corporal e comprimento da carapaça.
Subject(s)
Amazonian Ecosystem , Astacoidea , EstuariesABSTRACT
This study records, for the first time, the occurrence of all four male morphotypes in a population of Macrobrachium amazonicumfrom a continental environment, with an entirely freshwater life cycle. The specimens studied came from the Tietê River, state of São Paulo, Brazil, and were collected in a lotic environment downstream from Ibitinga Dam. This population was compared with other continental populations, including a population from the dam itself, collected in a previous study. Four samples of 30 minutes were taken monthly, using a trap, from January to April 2011. Each male specimen was measured with respect to seven body dimensions as follows: carapace length (CL), right cheliped length (RCL), dactyl length (DCL), propodus length (PPL), carpus length (CRL), merus length (ML) and ischium length (IL). The relative growth was analyzed based on the change in growth patterns of certain body parts in relation to the independent variable CL. The four male morphotypes proposed for the species were found using morphological and morphometric analyses. Different biological characteristics were found between the populations studied. The male population of the lake of Ibitinga and from Pantanal presented mean sizes and number of morphotypes lower than the population studied here. These differences seem to be closely related to ecological characteristics of the environments inhabited by these populations. Our results supported the hypothesis that coastal and continental populations of M. amazonicum belong to the same species.
Este estudo registra, pela primeira vez, a ocorrência dos quatro morfotipos de Macrobrachium amazonicum em uma população continental com ciclo de vida totalmente dulcícola. Os camarões são provenientes do Rio Tietê, estado de São Paulo, Brasil, e foram coletados em um ambiente lótico abaixo da Barragem de Ibitinga. Essa população foi comparada com outras populações provenientes de ambientes continentais, incluindo a de um estudo anterior na represa da barragem de Ibitinga. Quatro amostras de 30 minutos foram realizadas mensalmente, utilizando armadilhas, de janeiro a abril de 2011. Cada indivíduo macho foi mensurado em relação a sete dimensões corporais, sendo elas: comprimento da carapaça (CL), comprimento total do quelípodo direito (RCL), comprimento do dáctilo (DCL), comprimento do própodo (PPL), comprimento do carpo (CRL), comprimento do mero (ML) e comprimento do ísquio (IL). O crescimento relativo foi analisado de acordo com as mudanças nas taxas de crescimento de determinadas partes do corpo em relação à variável independente CL. Os quatro morfotipos descritos para a espécie foram encontrados, utilizando análises morfológicas e morfométricas. Diferentes características biológicas foram encontradas entre a população estudada e as demais, incluindo a proveniente do reservatório. A população de machos da represa de Ibitinga e do Pantanal apresentaram tamanhos médios e número de morfotipos inferiores aos da população estudada aqui. Essas diferenças parecem estar intimamente relacionadas às características ecológicas dos ambientes onde estas populações estão inseridas. A hipótese de que populações costeiras e continentais de M. amazonicumpertençam à mesma espécie é suportada por nossos resultados.
ABSTRACT
This study records, for the first time, the occurrence of all four male morphotypes in a population of Macrobrachium amazonicum from a continental environment, with an entirely freshwater life cycle. The specimens studied came from the Tietê River, state of São Paulo, Brazil, and were collected in a lotic environment downstream from Ibitinga Dam. This population was compared with other continental populations, including a population from the dam itself, collected in a previous study. Four samples of 30 minutes were taken monthly, using a trap, from January to April 2011. Each male specimen was measured with respect to seven body dimensions as follows: carapace length (CL), right cheliped length (RCL), dactyl length (DCL), propodus length (PPL), carpus length (CRL), merus length (ML) and ischium length (IL). The relative growth was analyzed based on the change in growth patterns of certain body parts in relation to the independent variable CL. The four male morphotypes proposed for the species were found using morphological and morphometric analyses. Different biological characteristics were found between the populations studied. The male population of the lake of Ibitinga and from Pantanal presented mean sizes and number of morphotypes lower than the population studied here. These differences seem to be closely related to ecological characteristics of the environments inhabited by these populations. Our results supported the hypothesis that coastal and continental populations of M. amazonicum belong to the same species.
Este estudo registra, pela primeira vez, a ocorrência dos quatro morfotipos de Macrobrachium amazonicum em uma população continental com ciclo de vida totalmente dulcícola. Os camarões são provenientes do Rio Tietê, estado de São Paulo, Brasil, e foram coletados em um ambiente lótico abaixo da Barragem de Ibitinga. Essa população foi comparada com outras populações provenientes de ambientes continentais, incluindo a de um estudo anterior na represa da barragem de Ibitinga. Quatro amostras de 30 minutos foram realizadas mensalmente, utilizando armadilhas, de janeiro a abril de 2011. Cada indivíduo macho foi mensurado em relação a sete dimensões corporais, sendo elas: comprimento da carapaça (CL), comprimento total do quelípodo direito (RCL), comprimento do dáctilo (DCL), comprimento do própodo (PPL), comprimento do carpo (CRL), comprimento do mero (ML) e comprimento do ísquio (IL). O crescimento relativo foi analisado de acordo com as mudanças nas taxas de crescimento de determinadas partes do corpo em relação à variável independente CL. Os quatro morfotipos descritos para a espécie foram encontrados, utilizando análises morfológicas e morfométricas. Diferentes características biológicas foram encontradas entre a população estudada e as demais, incluindo a proveniente do reservatório. A população de machos da represa de Ibitinga e do Pantanal apresentaram tamanhos médios e número de morfotipos inferiores aos da população estudada aqui. Essas diferenças parecem estar intimamente relacionadas às características ecológicas dos ambientes onde estas populações estão inseridas. A hipótese de que populações costeiras e continentais de M. amazonicum pertençam à mesma espécie é suportada por nossos resultados.
Subject(s)
Animals , Female , Male , Palaemonidae/anatomy & histology , Palaemonidae/classification , Brazil , Fresh Water , Life Cycle Stages/physiology , Palaemonidae/physiology , Reproduction/physiologyABSTRACT
Erythromycin A (EA) is now one of antibiotics limited in seafood products in general and in giant freshwater prawns (Macrobrachium rosenbergii) and tilapia (Oreochromis niloticus) in particular while exporting to the US, EU, Japan, Canada. There are many methods used for analyzing this antibiotic in these aqua species such as ELISA, HPLC, LC-MS/MS, GC-MS, etc. A new sensitive analytical approach for determination of erythromycin A using stripping square wave voltammetry at the slowly dropping mercury electrode was primarily developed and validated to quantify this antibiotic with simple and short time analysis, not too pure analytical solutions, not high price equipments. Electrochemical signals were measured at potential wave -1430 mV. The optimal experimental parameters for the method were: supporting electrolyte ammonium acetate 0.1 M, pH 8.0, the solvents for dissolving erythromycin standard: acetonitril, Vstart = -400 mV, Vstop = -1700 mV, Vstep = 6 mV, Vpulse = 40 mV, Tdrop = 5000 ms , Velctrolise = -1100 mV, Telectrolyse = 5 s. The method showed high recovery (85.07 ÷ 96.50 %), high sensitivity (lower limit of detection, LoD = 0.57 μg kg-1 in giant prawn and LoD = 0.52 μg kg-1 in tilapia) and high precision (RSD 0.91 ÷ 2.1 %) as well as excellent linearity (r2 adjusted ≥ 0.99999).