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Objective To develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to simultaneously detect the contents of gallic acid, syringin, phellodendrine, aesculin and rhein in the gallnut lotion. Methods An UPLC- MS/MS method was established. Separation was performed on an Agilent Poroshell 120 EC-C18(2.1 mm×150 mm, 2.7 μm)with a gradient mobile phase system of 0.2% formic water-acetonitrile solution. The flow rate was 0.3 ml/min. The temperature of column was 30 ℃. The injection volume was 2 μl. The MS detection was in dynamic MRM mode. Results gallic acid, syringin, phellodendrine, aesculin and rhein were successfully separated using this method, with good linear relationship as the ranges of 153.8-15380、10.31-1031、5.265-526.5、50.70-5070、1.054-105.4 ng/ml, respectively. The precision, repeatability, stability and recovery were good. Conclusion This UPLC-MS/MS method is stable, rapid, and reproducible., It is suitable for detecting the contents of gallic acid, syringin, phellodendrine, esculetin and in the gallnut lotion.
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Objective:To explore the influence of gallnut extract as a root canal irrigants in the curative effect of chronic periapical periodontitis,and to clarify its mechanism.Methods:A total of 192 patients diagnosed with chronic periapical periodontitis were selected and randomly divided into control group (distilled water),hydrogen peroxide group,sodium hypochlorite group and gallnut extract group (n=48).In the process of root canal treatment,four irrigants were used respectively,then the curative effects 1 week,6 months,12 months after treatment were observed and compared.Results:After root filling treatment,the cold and heat stimulation of the tooth was insensitive in clinical examination,percussion (-),loose degree≤ Ⅰ °,no swollen gums,bleeding on probing (-),no fistula,normal function of the exercise of chewing,decreased or disappeared transmission area in periapical bone in X-ray photo,and those metioned above were considered effective treatment.After 6 months oftreatment,the effective rate in gallnut group was 89.4％,and there were no statistically significant differences compared with hydrogen peroxide group (91.1％) and sodium hypochlorite group (91.5 ％) (P＞0.05),but there was significant difference compared with control group (67.4％) (P＜0.05).After root filling treatment for 12 months,the effective rate in gallnut group was 83.7％ and there were no statistically significant differences compared with hydrogen peroxide group (83.3％)and sodium hypochlorite group (87.8％) (P＞0.05),but the ratio of tooth from improvement to recovery in gallnut group was higher than those in hydrogen peroxide group and sodium hypochlorite group.Conclusion:Gallnut extract as a root canal irrigants has good antibacterial and cleaning effect,and can achieve the aim of curing the chronic root periapical inflammation and preventing the infection.
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Objective: To explore the influence of gallnut extract as a root canal irrigants in the curative effect of chronic periapical periodontitis, and to clarify its mechanism. Methods: A total of 192 patients diagnosed with chronic periapical periodontitis were selected and randomly divided into control group (distilled water), hydrogen peroxide group, sodium hypochlorite group and gallnut extract group (n=48). In the process of root canal treatment, four irrigants were used respectively, then the curative effects 1 week, 6 months, 12 months after treatment were observed and compared. Results: After root filling treatment, the cold and heat stimulation of the tooth was insensitive in clinical examination, percussion (-), loose degree≤ I°, no swollen gums, bleeding on probing (-), no fistula, normal function of the exercise of chewing, decreased or disappeared transmission area in periapical bone in X-ray photo, and those metioned above were considered effective treatment. After 6 months of treatment, the effective rate in gallnut group was 89.4%, and there were no statistically significant differences compared with hydrogen peroxide group (91. 1%) and sodium hypochlorite group (91. 5%) (P>0. 05), but there was significant difference compared with control group (67.4%) (P0. 05), but the ratio of tooth from improvement to recovery in gallnut group was higher than those in hydrogen peroxide group and sodium hypochlorite group. Conclusion: Gallnut extract as a root canal irrigants has good antibacterial and cleaning effect, and can achieve the aim of curing the chronic root periapical inflammation and preventing the infection.
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The main objective was to research the process of gallnut suppository preparation with its water extract as the main drug, and evaluate its irritation to rectal mucosa. gallnut extract was obtained by decocting method, and its suppository preparation was obtained by fusion method with semi-synthetic aliphatic esters and rose flower oil as the matrix. Weight difference and in vitro melting time limit of the suppository were assayed and UV-Vis was used to determine the contents of polyphenols, tannin and saccharide. The irritation to colon mucosa was evaluated after successive administration of 14 days to New Zealand white rabbits. Finally, the prescription compositions were determined: semi-synthetic aliphatic esters and rose flower oil with the ratio of 2:1 as the proper matrix, with the drug loading of 54%. The prepared suppository was brown, conical and smooth. The weight difference was (1.43±0.03) g, with an average melting time limit of (17±2) min. The Contents of Polyphenols, tannic and polysaccharide were 332.4, 245.0, 3.3 mg•g-1 respectively in each suppository. The results also showed that the continuous administration had no irritation to rectal mucosa. It can be concluded that the suppository was an acceptable administrate form, whose preparation process was easily controlled, and with no irritation to rectum mucosa.
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Objective To study the inhibitory effect of Gallnut on the biofilm of Candida albicans. Methods MTT assay was used to determine the inhibitory effect of Gallnut on biofilm formation and mature biofilm. RT-PCR was used to determine the effect of Gallnut on the expressions of ALS1 and CPH1 genes. Results Gallnut has a stronger inhibitory effect on Candida albicans biofilm formation, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) for inhibition of biofilm formation both are 128mg/mL. Gallnut also has a strong inhibitory effect on the mature biofilm of Candida albicans, the minimum inhibitory concentration (MIC) is 256mg/mL. Gallnut could significantly reduce the gene expressions of ALS1 and CPH1. Compared with the control group, 64 mg/mL Gallnut act on Candida albicans after 16h, the gene expression of ALS1 and CPH1 decreased by 79.2% and 82.0% respectively. Conclusion Gallnut has a significant inhibitory effect on the formation and maturation of Candida albicans biofilm. The inhibitory effect of Gallnut on the adherence and invasion of Candida albicans is mainly through inhibiting the expressions of ALS1 and CPH1 genes.
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Objective Study on the inhibitory effect of gallnut extract extract on MRSA β-lactamase. Methods Determination of inhibitory effect of gallnut extract on MRSA3002 by TTC method. β-lactamase was repeated by freezing and thawing method . Synergistic effect of gallnut extract and gentamicin was detected by TTC. Results The MIC and MBC of MRSA3002 by gallnut extract were 8mg/mL and 32mg/mL.Gallnut extract can reduce strains of β-lactamase activity,the MRSA300224h 1/2MIC after the effect of gallnut extract, beta lactam enzyme activity inhibition compared with the control group there were significant differences (P<0.01),compared with the positive control group, the difference was not significant. Synergistic effect of gallnut extract and gentamicin can significantly reduce the MIC of MRSA3002. Conclusion Gallnut extract can reduce β-lactamase activity recovery sensitivity of drug-resistant bacteria.
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Objective To investigate the inhibition mechanism of gallnut on biofilm formation by MRSA 41577.Methods TTC assay was used to detect inhibitory effects of biofilms formation and mature biofilms.The of PIA on biofilm formation was studied using Congo red agar method.Micro-Ultraviolet Spectrophotometer was used to detect inhibitory effects of the release of eDNA.The influence for Baicalein on icaA and cidA gene expression were detected by RT-PCR method.Results The inhibitory concentration (MIC) and minimum bactericidal concentration (MIC) of MRSA 41577 BF were 0.5 mg/mL and 1 mg/mL, respectively.The inhibitory effect of galla on MRSA 41577BF formation and mature BF was significantly inhibited.Inhibition of MRSA 41577,the MIC and MBC of mature BF were 4 mg/mL and 16 mg/mL.Congo red test results show that Galla can inhibit the synthesis of MRSA 41577 PIA, and the concentration was dose-dependent.The results showed that gallnut could inhibit the release of MRSA 41577 eDNA, and the release amount of eDNA was 3.61μg/OD595 and 11.91μg/OD595 , respectively, when the concentration of gall was 1/2MIC.The release of eDNA was reduced by 69.7% (P<0.01).The expression of icaA and cidA genes in the control group was 9.7% and 6.67%, respectively.The expression of icaA and cidA in the control group was significantly lower than that in the control group ( icaA and cidA, and cidA gene expression were 100%, the expression of icaA and cidA genes were reduced by 90.3%and 93.3%, respectively (P<0.01).Conclusion The inhibitory effect of gallnut on the biofilm of MRSA 41577 is mainly through inhibiting the expression of icaA and cidA genes, and then affecting the synthesis of PIA and the secretion of eDNA .
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Objective To study the inhibitory effect of Gallnut on the biofilm of Candida albicans. Methods MTT assay was used to determine the inhibitory effect of Gallnut on biofilm formation and mature biofilm. RT-PCR was used to determine the effect of Gallnut on the expressions of ALS1 and CPH1 genes. Results Gallnut has a stronger inhibitory effect on Candida albicans biofilm formation, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) for inhibition of biofilm formation both are 128mg/mL. Gallnut also has a strong inhibitory effect on the mature biofilm of Candida albicans, the minimum inhibitory concentration (MIC) is 256mg/mL. Gallnut could significantly reduce the gene expressions of ALS1 and CPH1. Compared with the control group, 64 mg/mL Gallnut act on Candida albicans after 16h, the gene expression of ALS1 and CPH1 decreased by 79.2% and 82.0% respectively. Conclusion Gallnut has a significant inhibitory effect on the formation and maturation of Candida albicans biofilm. The inhibitory effect of Gallnut on the adherence and invasion of Candida albicans is mainly through inhibiting the expressions of ALS1 and CPH1 genes.
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Objective To investigate the compound rhubarb gallnut gargle combined periodontal treatment effect on inflammatory factors in patients with chronic periodontitis.Methods 76 patients with chronic periodontitis from June 2014 to May 2015 in our hospital were selected and randomly divided into two groups,given periodontal treatment as control group,compound rhubarb gallnut gargle combined periodontal treatment as observation group,38 cases in each group,two groups were detected after treatment of gingival crevicular fluid levels of inflammation.Results After treatment,the gingival crevicular fluid of tumor necrosis factor-α(alpha TNF-α),high sensitive C-reactive protein(hs-CRP)and interleukin three(IL-6,IL-1 beta and IL-8)of two groups were significantly lower than those before treatment,and the observation group of inflammatory cytokine levels were significantly lower than the control group,the difference was statistically significant(P<0.05).The gingival index(GI)and gingival bleeding index(SBI)of the two groups were were lower than those before treatment,and the GI and SBI in the observation group were lower than those in the control group,the difference was statistically significant(P<0.05).Conclusion In the treatment of chronic periodontitis,compound rhubarb gallnut gargle combined periodontal basic treatment effect,can significantly reduce the level of inflammation.
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Objective To observe the effect of externally-applied gallnut powder on Shenque integrated with orally-administeredWuhong Decoctionon sweat syndrome of cancer patients and summarize its nursing points. Methods Sixty patients were randomly divided into the control group and the therapeutic group, each with 30 patients. The therapeutic group was treated with externally-applied gallnut powder on Shenque and medication of'Wuhong Decoction'apart from the conventional treatment which was only used in the control group. The two groups were compared in terms of the situation of spontaneous sweat and night sweat and score of dizziness. Result The effects of spontaneous sweat and night sweat in the therapeutic group were better than those of the control group (P<0.05). Conclusions Externally-applied Gallnut powder on Shenque combined with medication ofWuhong Decoction can be effective in the treatment of sweat syndrome of cancer patients. It can help them to regain their physical power and then strengthen their resistance against diseases and finally enhance their quality of life.
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Objective:To observe the inhibition of Chinese gallnut on bacteria in plaque biofilms by the use of artificial mouth.Methods:Water extract of Chinese Gallnut was prepared and liquid media two-fold dilution method was used to determine the minimal inhibition concentration(MIC)of the drug on S.mutans,A.viscosus,S.sanguis and S.oralis.Individual bacteria plaque biofilms in artificial mouth were created.The inhibition of Chinese gallnut on the bacteria in plaque biofilms was studied by colony counting.Results:In bacteria suspension gallnut water extract inhibited the growth of the four kinds of bacteria.The MIC of the extract on S.mutans,A.viscous and S.sanguis was 64 mg/ml,that on S.oralis 8 mg/ml.The plaque biofilms formed by the four kinds of bacteria could be inhibited by 2 mg/ml-128 mg/ml of the extract dose-dependently.At the highest tested conentration(128 mg/ml)the gallnut water extract could not completely clear up the colonyforming unite(CFU)of the becteria.Conclusion:Chinese gallnut water extract can inhibit the growth of bacteria in suspension and in plaque biofilms,the bacteria in biofilms may have stronger resistance to the extract.
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Objective:To observe the effects of gallnut water extract on the vitality of plaque biofilms.Methods:The oral plaque biofilms were obtained through bonding the enamel fragments to the bucca of the mindibular first molar for 24 h.The two groups of the biofilms were treated by gallnut water extract at 6 mg/ml and saline(control) for three minutes respectively.The effect of gallnut water extract on the vitality of plaque biofilms was observed by Ethidium bromide/Fluorescein diacetate (EB/FDA) staining and confocal laser scan microscope(CLSM).Results:All specimens had early biofilm formed,The percent vitality of the plaque biofilms treated with gallnut water extract and the control was 37.10?9.63 and 60.78?7.60 respectively(P