Study on quality control of melittin by SDS-PAGE method / 中草药

Object To establish a SDS PAGE method on quality control of melittin. Methods Melittin was extracted and purified from bee venom by gel chromatography. Purity and molecular mass of melittin were determined by SDS PAGE. Results In the gelatinous board, the sample was a single peptide chain with a molecular mass of about 2 800 dalton as revealed by SDS PAGE. The purity was (90 59?0 85)%, which was in parallel with the result tested by HPLC. Conclusion SDS PAGE is accurate, reliable and simple for the determination of melittin.