Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp.

Bacterial are well known for their ability to excrete enzymes into the environment. Bacillus sp. being industrially important organisms produces a wide variety of extra-cellular enzymes including proteases. Bacillus sp. isolated from local marine samples collected from Saudi Arabia for produced protease enzyme. The aim of this work was to evaluate protease production by different bacterial isolated from marine. Six bacterial isolates were screened for protease production. Two protease producing isolates, No.2 and No.3 were selected on the basis of gelatin hydrolyses. When the extracellular protease were examined using fermentation production medium, the date recorded the highest extracellular protease enzyme reached to (243 U/ml) by isolate No. 2. Finally the selected isolate was identify according to the morphological characteristics and named Bacillus sp No.2 EHN.

Formation of proteases from newly isolated strain isolated from Saudi Arabia.

Comparative study was carried out on the production of protease using isolate from moraine. The isolates were screened on gelatin plates for gelatin hydrolyses by the different isolates. Among the all tested isolate No. 7 EHN produced the highest activity. while under the fermentation conditions after 3 days the maximum yield of protease was 277 U/ml. The protease production from marine isolate could be commercially used for applications purpose.