Gender Determination in Pakhtun Pakistani Population Using Dental Arch Dimensions: A Digital Model Study

@#Introduction: In circumstances where the ante mortem list is unknown, gender determination would exclude onehalf of the population, aid in a more precise search of the ante mortem records. This study aims to formulate gender prediction models in the Pakhtun Pakistani population using digital dental arch dimensions. Methods: Data collection and analysis of the dental casts were conducted on 128 subjects, 64 males and 64 females from the Pakistani population. The mean age of the subjects was 19.2 years old. Several linear dental arch dimensions were measured and recorded for both upper and lower arches. Results: It was found that gender differences in linear arch dimensions were statistically significant for both males and females (p<0.05); in which the arch dimensions for the males were larger than the arch dimensions for the females. Stepwise discriminant function analysis found that the highest discriminant power of the variables was present within the inter-second premolar width for the upper arch and inter-molar width for the lower arch. These variables significantly contributed to gender variance. Moreover, the prediction of 67.2% of original grouped cases for the upper arch and 66.4% of cross-validated group cases was correct. Similarly, the correct prediction was made on 64.8% of original grouped cases for the lower arch and 64.1% of cross-validated group cases. Conclusion: The dental arch dimensions were larger among the males compared to the females. Prediction models obtained in this study were moderately strong predictors which may be used as an adjunct to predict gender.

Use of pulp tissue of deciduous teeth for gender determination - A comparative molecular analysis study

Aim: The present study aimed to evaluate the effectiveness of pulp tissue collected from deciduous teeth for the determination of gender using polymerase chain reaction (PCR). Materials and Methods: 140 extracted deciduous teeth were selected. The control group comprised 20 teeth that were subjected to DNA analysis immediately. Whereas Group I and Group II consisted of 60 teeth which were stored in the open environment and salt water, respectively, for a period of 3, 9, and 15 months. DNA was isolated and quantified followed by the amplification of X and Y chromosomes by PCR and compared with the actual gender of the child. The data were analysed using the Shapiro?Wilk test, the independent sample t?test, paired t?test, and the Chi?square test. Result: The PCR analysis results of Group I showed a more correct interpretation of gender as compared to Group II on storage for a period of 15 months. The PCR analysis results of the Control group showed a 100% accuracy rate as compared to the samples in Groups I and II. Conclusions: Gender could be effectively determined from the samples evaluated immediately after extraction. But the period of storage and the method of storage conditions affected the quality of isolated DNA and thus decreased the ability of gender determination

Accuracy of mid-trimester ultrasound scan in foetal gender determination at the University College Hospital, Ibadan Nigeria

Objectives: This study aimed to determine the accuracy of prenatal sonographic gender determination during foetal anomaly ultrasound and the overall sensitivity pattern in our institution.Materials and Methods: A cross-sectional study of 520 consenting pregnant women who presented for foetal anomaly scans within a one-year period in our institution. The diagnostic accuracy of gender determination during the anomaly and delayed scans were determined by comparing the sonographic gender with the birth gender and calculating sensitivity, specificity, positive predictive value and negative predictive value. Data were analyzed using the Statistical Package for Social Sciences (SPSS) version 20. Descriptive statistics, frequencies, Mc-Nemar chi-square test were used at 5% level of significance. Results: The mean maternal age was 31.51 ±5.02years. Of the 520 consenting pregnant women studied, 16(6.0%) women were having twin gestation. Four hundred and ninety-seven (92.7%) genders were determined during foetal anomaly scan. The accuracy of the ultrasonography (US) examination performed by the resident doctors was 98.02% while the accuracy of the scan performed by the consultant radiologists was 100%. Overall, the accuracy of the gender determination on ultrasound was 98.69%. The general specificity and sensitivity of the US were 98.71 % and 98.68% respectively while the positive and negative predictive value were 99.01 % and 98.29% respectively. Conclusion: The accuracy of ultrasound examination in detecting foetal gender during foetal anomaly ultrasound is high with equally high predictive values and therefore it is recommended as a mandatory variable during anomaly scans. There is need for continuous training of resident doctors or operators in lower cadre to improve their competency in foetal gender determination.

Lip Print Analysis in Malaysian Chinese Population (Klang Valley): Lipstick-Cellophane Tape Technique (Analisis Cap Bibir Populasi Cina Malaysia di Lembah Klang: Teknik Gincu-Pita Selofan) / Jurnal Sains Kesihatan Malaysia

@#Cheiloscopy is recognized as a tool for personal identification due to its strength in criminalistics. In this study, the lip print of Malaysian Chinese population in Klang Valley was used for gender determination using lipstick-cellophane technique. 412 subjects (203 males and 209 females) were selected conveniently. Lipstick was first applied on the lips and the lip print was lifted using cellophane tape prior to pasting them onto a plain A4 paper and analysis using magnifying glass. Six topography areas of lip prints were classified using Suzuki and Tsuchihashi’s classification, including upper left, upper middle, upper right, lower right, lower middle and lower left. Chi-square test showed that all lip sections were significantly different from each other (p < 0.05). Type II was the dominant pattern in four lip sections for both genders: upper left (male 81.3%, female 57.4%), upper right (male 71.9%, female 42.9%), lower left (male 98.0%, female 90.0%) and lower right (male 96.1%, female 86.1%). Type IV was the dominant pattern for both middle part of the lips; upper middle (male 56.2%, female 50.7%) and lower middle (male 62.6%, female 50.2%). The results showed that gender can be differentiated based on lip print patterns. This finding suggested that cheiloscopy could be used in aiding personal identification for forensic investigation.

Morphometric Variation of Adult Human Clavicle - A Tool for Gender Determination

Introduction: Bones can reflect the basic framework of human body and provide valuable information about the biological identity of the deceased. They often, survive the morphological alteration, decay/mutation and decomposition insults. An in depth, knowledge and analysis of variation in clavicular length, midclavicular circumference and weight is imperative for both clinical and forensic anthropological perspectives. Objectives: The present study was done to determine morphometric variability of adult human clavicles by using length, weight and midclavicular circumference parameters. Materials and Methods: 300 pairs of adult human clavicles of known sex obtained from cadaveric dissection and bony collections of anatomy department were utilized for the study. The length, weight and midclavicular circumference were measured. The data obtained was statistically analysed. Results: Gender specific statistical analysis for length, weight and midclavicular circumference was found to be highly significant. The side specific significance was found only in male and in female clavicles for weight and midclavicular circumference respectively. No side specific significance found for length of clavicle. Conclusions: The present study provides morphometric data for North Indians and compares observations with other populations. The study establishes that length, weight and midclavicular circumference of clavicle have a dimorphic potential. This fact can play a decisive role in forensic identifications and clinical interventions. The results are of immense significance for anatomists, orthopedic surgeons and the forensic anthropologists in their professional endeavors.

Radiomorphometric Evaluation of Orbit and Inter-Orbital Distance for Gender Determination

Introduction: Anthropometry deals with obtainingmeasurements of anatomical structures to assist in identifyingthe age, stature, gender and several aspects related to individualor ethnic group. Orbital measurements and inter-orbitaldistance are one of the craniofacial parameters that couldbe used in gender identification in terms of anthropologicalstudies. Study aimed to measure the width of the orbitalapertures on Postero-anterior skull radiographic view, tomeasure the height of the orbital apertures on Postero-anteriorskull radiographic view, to measure the interorbital distanceon Postero-anterior skull radiographic view and to determinegender from radiomorphometric evaluation of orbit and interorbital distanceMaterial and Methods: In this study, a total of 100retrospective postero-anterior radiographs were selected fromdepartment’s collection using KODAK-8000 panoramic andcephalometric system following radiation protection protocol.Subjects were in the age range of 18 to 50 years. The maximumwidth and height of orbit and inter-orbital distance of theorbits were measured using masterveiw software (Version4.5.1). The data obtained was analyzed by using student“t” test.Results: The mean orbital width in males was 4.91 andfemales was 4.97. The observed mean difference wasstatistically significant (P-0.005). The mean orbital height inmales was 4.96 and females was 4.86. The observed meandifference was statistically significant (P-0.001) The meaninter-orbital distance in males was 3.37 and females was 3.00.The observed mean difference was statistically significant(P-<0.001)Conclusion: Hence we conclude that orbit and inter-orbitaldistance can be used in determining the gender.

To Correlate The Age And Gender Between Living Female Children And Adult Through Measurement Of Different Segment Of Sternum Bone In North Population Of India

Introduction: The ideal rule of identification of sex and age is a sever need in medico legal cases. Sternum ismuch more appropriate for detection of sex and age of human beings separately from racial characters.Method: This study has been conducted on the total 100 subjects, 50 female (0-15 yrs) and 50 female (16-60yrs)of healthy people (living children & adults) from general population of north India as well as in and around theLucknow of Uttar Pradesh. The technique described by Ashley GT -1956b was used for measurement of sternum.Discussion: on the basis of observation and result in my present study it was fully supported and agree of theprevious researchers, who has done the work in measurement of sternum bone in cadaver and skeletal of human,because they told that the sterna has distinguish in different zone of India.Result: Comparing the mean length, Student’s t test showed significantly different and higher (8.1%) length ofmanubrium in 16-60 yrs female living adults as compared to 2-15 yrs female living children (44.47 ± 5.79 vs.48.38 ± 5.71, t=3.39, p=0.001).Conclusion: Different segment of sternum bone significantly different and higher length of manubrium, becausethe growth of sternum segment was completely develop in adult, but the development in children female iscontinuous in ratio of adults female.

Evaluation of Gender in Living Male and Female Children Through the Measurement of Sternum Bone in the Population of Uttar Pradesh

Introduction: Sex evaluation from the skeletal remains is of very much important for medicolegal aspects in the field of medical science. On the basis of previous researcher, I have been planned to verify the existrnal data and establish them in reference to population in and around Lucknow. With the help of this study we were distinguishes the male and female sternum bone in children. Subjects and Methods: The technique described by Ashley GT -1956b was used for measurement of sternum. Each linear measurement has been taken thrice on the anatomical position of the sternum using Mitutoyo-digital vernier calipers to the nearest millimeter with precision of 0.01 mm. Results: Discussion: The previous researchers told that the sternum has distinguished in different zone of India, and it is shorter than European country. Our findings of manubrium were 49.45±3.45 mm for male and 44.47± 5.79 mm for female children respectively. The study done by previous researcher was provided full support to our present finding. Conclusion: The comparison of mean length of manubrium, mesosternum and sternum between 2 to 15 yrs, female and male sternum between two groups, significantly different and higher length of manubrium, mesosternum and sternum in males as compared to females children.

Preliminary investigation of gender assignment in 46,XY disorders of sex development with severe male undermasculinisation / 中华儿科杂志

Objective@#To explore the feasibility of gender assignment in 46,XY disorders of sex development (DSD) with severe undermasculinisation mainly based on molecular diagnosis.@*Methods@#A retrospective study of 45 patients of 46, XY DSD with severe undermasculinisation were admitted between November 2015 and October 2018 at Children′s Hospital, Zhejiang University School of Medicine. The initial social gender were all female, of whom the external genital manifestations were Prader 0 to 2; the degree of masculinity was scored using external masculinisation score (EMS); the position and development of the gonads were examined by ultrasound, cystoscopy and laparoscopy, also including assessing the development of the Wolffian tube and the Müllerian tube. The level and ratio of testosterone to dihydrotestosterone before and after hCG stimulation were evaluated for the function of Leydig cell and 5α-reductase-2. Gender role scales and sandbox games were used to assess gender role behavior. Genital sensitivity to androgen stimulation was assessed; A panel including 163 genes related to gender development were determined by second-generation sequencing in all 45 patients. Finally, a multidisciplinary team (MDT) makes a gender assignment after a comprehensive analysis mainly based on the molecular etiological diagnosis.@*Results@#Thirty-nine out of 45 patients (87%) had an identifiable genetic etiology, and the remaining 6 (13%) were negative for genetic testing. Forty-five patients had EMS less than or equal to 3 points. Sexual psychological assessment was performed in 39 patients, with male dominance in 24 (62%) and female dominance in 15 (38%). The gender assignment was 23 cases (51%) for male and 19 cases (42%) for female, and 3 cases (7%) were not completely determined.@*Conclusions@#Molecular diagnosis provides a strong basis for appropriate gender assignment of 46, XY DSD children with severe undermasculinisation. Based on molecular diagnosis, each DSD should be analyzed by professional MDT to analyze the clinical symptoms/signs, gonadal development, gonad tumor risk, external genital morphology, sexual psychological assessment, potential fertility opportunities, parental views, Social and cultural factors, etc. make appropriate gender assignment.

Determination Of Age And Gender Using Bigonial Width - An Orthopantomographic Study

INTRODUCTION;. Mandible plays a major role in determination of age and gender which is important in forensic and medico legal cases. Skull is the most dimorphic portion of the skeleton after pelvis and mandible is dimorphic, largest and strongest bone of the skull. Hence, using digital panoramic radiographs this study was conducted in Telangana population to evaluate the usefulness of different linear measurements of ramus in determination of age and gender. MATERIALS AND METHOD: 150 panoramic images of dentate subjects with an age ranging from 20-70yrs old were selected for the present study. The Bigonial Width measurements were performed and further subjected to statistical analysis. RESULTS: It was seen that the mean value of the bigonial width was more in males than females. On comp, the mean value of bigonial width among different age groups, there was a significant difference among the mean values of bigonial width.

Evaluation And Comparison Of Various Methods Of Lip Prints In Gender Determination And Its Stability Over A Period Of Time

Context: Gender identification is one of the most important aspects in the forensic science. It is based onscientific principles involving dental records, fingerprints and DNA comparisons. Sometimes, it becomes necessaryto apply easy techniques like cheiloscopy. The wrinkles and grooves on the labial mucosa form a characteristicpattern called lip prints, the study of which is referred to as cheiloscopy.Aim: The present study was done to evaluate the accuracy of various methods of lip prints in gender determinationand its stability over a period of time.Subjects and Methods: It consists of 80 healthy individuals (40 males and 40 females) in the age group of 18 to25 years. Lip prints were taken by lipstick, latent and digital photography methods. Data was collected by twoobservers at 0 month and 6months to assess their stability in gender determination.Statistical Analysis Used: Chi-square test was used.Results: The results obtained showed an accuracy of 0% and 57.5% by observer I, 30% and 67.5% by observer IIat 0 and 6th month in gender determination. The measurement of agreement in stability of lip prints was found tobe 27.8% by observer II.It was found that digital photography being the accurate method followed by lipstick andlatent methods.Conclusion: This study revealed that lip prints can be used for determining the gender of an individual.

Application of maxillary canine pulp volume / 中国法医学杂志

Objective To explore the clinical application value of three-dimensional reconstruction of maxillary canine pulp volume /tooth volume (PV/TV)in individual gender determination.Methods There were 103 patients (51 males and 52 females) with CBCT imaging data from September 2015 to August 2016, Shanxi Medical University, department of radiology, Mimics 17.0 software was used to measure pulp volume of maxillary canines and tooth volume, and to calculate the ratio of pulp volume/tooth volume.The data were processed by SPSS16.0 statistical software and Fisher discriminant method. The gender determination function was obtained and Cross -Validated method for performance evaluation. Results When gender determination was determined by tooth volume (TV) and pulp volume/tooth volume (PV / TV) as the study index, the gender discriminant function was Y=0.009(TV)+28.896(PV/TV) - 6.962, Cross-validated method for the effect of evaluation, to determine the compliance rate: 51 males, the correct rate of 64.7%;52 female, the correct rate of 78.8%. Conclusion The tooth volume (TV) and pulp volume/tooth volume (PV/TV)can be used as a research indicator of gender inference, it provides a new method and avenue for the gender determination in forensic medicine.