ABSTRACT
The imprinted gene H19, one of the maternally imprinted gene which had been found long time ago, is highly expressed in early embryo. It mainly concentrates in endoderm and mesoderm which plays an important role in regulating the development of embryos and in control of off spring behavior. More and more studies have shown that the alterations of methylation status in CpG islands of H19 gene imprinted control region will lead to the decline of male sperm quality and female oocyte quality, then affecting human fertility. Imprinting gene H19 is also related to the development of the embryo. This article reviews the progress of methylation of the imprinted gene H19 in assisted reproductive technology.
ABSTRACT
Since human imprinted genes in 11P15.5 was found,it had been proved that various human physiological abnormality associated with it.As one of the first identified imprinted non-coding RNA genes,more attention is paid to lncRNA H19 by many scholars not only because it's linked with Beckwith-Wiedemann syndrome (BWS) and Russell syndrome (SRS),but also H19 gene is the only imprinted gene belonging to long non-coding RNA related with a variety of tumors which can also produce microRNA675 and lncRNA 91H coming from H19 gene antisense-coding.This paper will review possible mechanisms of H19 gene in tumorigenesis process and its research status in Cancer therapy from four aspects:imprinting feature,single nucleotide polymorphisms,lncRNA character and its possible action signal axis in cancer.
ABSTRACT
Objective@#To investigate the influence of high fat diet-induced obesity (HFDIO) on the differentially methylated region (DMR) of the imprinted gene and global genome methylation of sperm DNA.@*METHODS@#We performed bisulfite sequencing on the DMR of the imprinted gene and global genome methylation of sperm DNA in the mouse model of HFDIO.@*RESULTS@#No statistically significant differences were found between the HFDIO model and normal control mice in MEG3-IG (93.73 vs 97.26%, P = 0.252), H19 (98.00 vs 97.83%, P = 0.920), IGF2 (97.34 vs 96.25%, P =0.166), IGF2R (1.43 vs 1.11%, P = 0.695), PEG3 (0.19 vs 0.38%, P = 0.537), MEST (0.23 vs 0.68%, P = 0.315), NNAT (0.31 vs 0.00%, P = 0.134), or SNRPN (1.88 vs 3.13%, P = 0.628). A total of 8 942 DMRs were detected across the sperm genome (P <0.05). Gene functional enrichment analysis indicated that the enriched terms with the largest numbers of genes were the metabolic process (n = 1 482), RNA synthesis (n = 779), and transcription (n = 767).@*CONCLUSIONS@#The methylation level underwent no significant change in the DMRs of the imprinted genes from the mice with HFDIO, but the CG methylation of the genes involved in the metabolic process, RNA synthesis and transcription were significantly altered.