ABSTRACT
AIM:To explore the effects of Gax gene transfection on expressions of c-fos and c-jun mRNA and proliferation of pulmonary arterial smooth muscle cells (PASMCs) in rat under hypoxia. METHODS: PASMCs were transfected with Gax gene by Ad-Gax. Under normal oxygentention (21% O2) or hypoxia (2.5% O2) for 12 h condition, expressions of Gax mRNA and protein in PASMCs were detected by RT-PCR and immunocytochemistry. The expressions of c-fos and c-jun mRNA were evaluated by RT-PCR. [3H]-TdR incorporation was used to measure the PASMCs proliferation. RESULTS: The Gax overexpression in transfection group was confirmed by RT-PCR and immunocytochemistry. Under normal oxygentention or hypoxia, the c-fos and c-jun mRNA levels in transfection group were lower than those in the non-transfection group, respectively (P
ABSTRACT
Objective To investigate the role of proliferating cell nuclear antigen (PCNA) and cyclin dependent kinase 2 (CDK2) in inhibition of vascular smooth muscle cells (VSMCs) proliferation by gax gene. Methods After being transfected by AdCMV-gax, the expression of gax, PCNA and CDK2 in VSMCs were assessed. The effect of gax overexpressions on VSMCs proliferation was observed by 3 H-thymidine incorporation. Results After AdCMV-gax was transfected to VSMCs, the level of Gax protein expression was significantly higher than that before transfection. PCNA and CDK2 expressions were decreased after VSMCs were transfacted with AdCMV-gax. The level of 3 H-thymidine incorporation was decreased significantly in with AdCMV-gax transfected VSMCs compared with that in non-transfected VSMCs. Conclusion The mechanism of inhibition of VSMCs proliferation by gax gene is related with depression of expression of PCNA and CDK2.