Effects of Parental Dietary Exposure to GM Rice TT51 on the Male Reproductive System of Rat Offspring / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To evaluate the health effects of parental dietary exposure to GM rice TT51 on the male reproductive system of rat off spring.</p><p><b>METHODS</b>Rice-based diets, containing 60% ordinary grocery rice, MingHui63, or TT51 by weight, were given to parental rats (15 males/30 females each group) for 70 days prior mating and throughout pregnancy and lactation. After weaning, eight male offspring rats were randomly selected at each group and fed with diets correspondent to their parents' for 70 days. The effects of exposure to TT51 on male reproductive system of offspring rats were assessed through sperm parameters, testicular function enzyme activities, serum hormones (FSH, LH, and testosterone levels), testis histopathological examination, and the relative expression levels of selected genes along the hypothalamic-pituitary- testicular (HPT) axis.</p><p><b>RESULTS</b>No significant differences were observed in body weight, food intake, organ/body weights, serum hormone, sperm parameters, testis function enzyme ACP, LDH, and SDH activities, testis histopathological changes, and relative mRNA expression levels of GnRH-R, FSH-R, LH-R, and AR along the HPT axis.</p><p><b>CONCLUSION</b>The results of this study demonstrate that parental dietary exposure to TT51 reveals no significant differences on the reproductive system of male offspring rats compared with MingHui63 and control.</p>

EFFECTS OF THE GENETICALLY MODIFIED RICE ENRICHED WITH RESISTANT STARCH ON LARGE BOWEL HEALTH IN RATS / 营养学报

Objective To study the effects of the genetically modified(GM) rice enriched with resistant starch on large bowel health in rats. Method Forty-eight healthy male SD rats were randomly assigned into four groups based on body weight,and given with no-GM rice diet (No-GM), GM rice diet(GM), half-GM rice diet (Half-GM) and normal diet (ND), respectively. After fed for 5 w, 4 d feces samples were collected. At 6 w all rats were killed and the contents of cecum and colon were collected. Large bowel function was evaluated by determination of cecum and colon weight, their contents, pH value, short-chain fatty acids (SCFA), etc. Results GM group had similar body weight with ND and significantly less than No-GM. Compared with No-GM and ND, fecal bulk and moisture, cecum and colon weight and the cecum contents of GM and Half-GM were higher dose-dependently. The contents of SCFA in cecum, colon and feces dropped gradually. SCFA level of cecum and colon was higher significantly in GM and Half-GM compared with No-GM and ND except butyric acid of colon in Half-GM. In all groups, there were differences of acetic and propionic acid level in feces but no difference of butyric acid. Cecum and fecal pH value of GM and Half-GM dropped much significantly compared with No-GM and ND. Conclusion The genetically modified rice enriched with resistant starch can improve the large bowel health of rat.