ABSTRACT
AIM: To determine the content of ginsenoside Rg_1、Re and Rb_1 in Shenfu Injection (Radix et Rhizoma Ginseng Rubra,etc.) by HPLC-ELSD. METHODS: Separation was achieved on a Waters ODS column((4.6) mm?150 mm,5 ?m).Elution was carried out using a gradient of acetonitrile and water.Drift tube temperature:120 ℃,gas flow rate:1.8 mL/min. RESULTS: The calibration curves were linear in the range of 0.322-(3.22) ?g for ginsenoside Rg_1,0.290-2.90 ?g for ginsenoside Re,0.508-5.08 ?g for ginsenoside Rb_1. CONCLUSION: The method is simple,accurate,effective and feasible.It can be used to control the quality of Shenfu Injection.
ABSTRACT
AIM: To establish the quality sdandard for Xintongning Tablet(Radix et Rhizoma Ginseng,Radix et Rhizoma Salviae Miltiorrhizae,etc.) METHODS: TLC was used to indentify Radix et Rhizoma Salviae miltiorrhizae,Rhizoma Curcumae Longae and Fel Ursi Powder.The contents of ginsenoside Rg_1,Re were determined by HPLC. RESULTS: Radix et Rhizema Salviae miltiorrhizae,Rhizoma Curcumae Longa and Fel Ursi Powder could be indentified by TLC.The average recoveries of ginsenside Rg_1,Re were 97.18% and 96.74% respectively(n=5). CONCLUSION: The methods are reliable and reproducible.It can be used for quality control of Xintongning Tablets.