ABSTRACT
This study was designed to investigate the effects of pyroligneous liquor on oxygen radicals and their scavenger enzy-mes in the liver of Cri/Bgi CD rats (7 rats per group ). Male rats were fed a basic diet prepared in our Lab., PL-0 (Control ), PL-1, PL-25, PL-50 and PL-75 groups were prepared to be 0%, 1%, 25%, 50% and 75%with distilled water using pyrolig-neous liquor (35% of Choa Co. Ltd.), and were administrated orally for 8 weeks. Superoxide radical contents in liver mi-tochondria and microsomes were significantly decreased to 12-14%, 11-15%, respectively, in these PL-25 and PL-50 groups compared with the control group. Hydroxyl radical content in mitochondria and microsomes were markedly decreased to 12-20% and 17%, respectively, in these PL-25 and PL-50% groups compared with the control group. Hydro-gen peroxide content in mitochondria and microsomes were significantly decreased about 15-12% and 22-20% in liver of PL-25 and PL-50 groups compared with the control group. Mn-SOD and Cu/Zn-SOD activities in liver of PL-25 and PL-50 groups were remarkably increased to 15-25%, 11-16%, respectively, compared with the control group. GPx activities in mitochondria and microsomes were significantly increased in the liver of PL-25 and PL-50 groups compared with the control group. CAT activities in mitochondria and cytosol were significantly increased to 12-14%, 15-27%, respectively, in the liver of PL-25 and PL-50 groups compared with the control group. These results suggest that long term administra-tion orally of 25 and 50% pyroligneous liquor may effectively inhibit the formation of oxygen free radicals, and also scavenger enzyme activities significantly increase through the administration orally.