ABSTRACT
Crude glycosaminoglycan(CPG) from the whole viscera of Pinctada martensii was isolated with the two-enzyme hydrolysis and purified by the chromatograph.And the antitumor activity was studied with MTT colormetric assay. The CPG could be separated into two fractions(GAG-1, GAG-2) by the DEAE-52-cellulose ion exchange chromatograph. The contents of hexosamine of CPG,GAG-1, GAG-2 were 54.6%,50.0%,35.4%,respectively. The sulfate of GAG-2 was 13.8%. The inhibiting rates of CPG, GAG-1 and GAG-2 to HL-60 cells were 54.6%, 50.0% and 35.4%,respectively. The inhibition to HL-60 of 5-Fu could be increased by combined use with CPG, GAG-1 and GAG-2, GAG-1 was the main active fraction.
ABSTRACT
Objective To study the extraction and purification of glycosaminoglycans from Rhoqilema esculenta Kishinouye,optimize the enzymolysis process,and its antihyperlipidemia effects were studied.Methods Extraction of glycosaminoglycans(GAG) from Rhopilema esculenta Kishinouye by hydrolyzation method with papain,subtilisin and trypsin neutral proteinase,ethanol precipitation.The rude product of glycosaminoglycans(GAG) was purified and fractionated by the methods of adsorption,dialysis,ethanol precipitation and CTAB precipitation.The experimental hyperlipidemia mice induced by high fat forage were studied.The total cholesterol(TC) and triglyceride(TG) levels were determined.Results Optimum enzymolysis process: papain neutral proteinase1.5%,subtilisin neutral proteinase1.5%,treated for 5h;The result showed that the levels of TC and TG decreased to 6.83?1.09 mmol?L-1(P