Simultaneous determination of five constituents in Gastrodiae Rhizoma from seven growing areas by RP-HPLC / 中成药

AIM To develop a RP-HPLC method for the simultaneous content determination of five constituents in wild Gastrodia elata Blume,G.elata Bl.form.glauca S.Chow,G.elata Bl.form.elata and G.elata Bl.form.flavida S.Chow from seven growing areas (Zhaotong and Lijiang in Yunan,Lu'an in Anhui,Hanzhong in Shannxi,Dejiang in Guizhou,Guangyuan in Sichuan,and Yichang in Hubei).METHODS The analysis of Gastrodiae Rhizoma 70％ methanol extract was performed on a 35 ℃ thermostatic Waters Sunfire C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of methanol-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 270 nm.RESULTS Adenosine,gastrodin,4-hydroxybenzyl alcohol,4-hydroxybenzaldehyde and parishin A showed good linear relationships within their own ranges (r≥0.999 5),whose average recoveries were 99.34％-101.33％ with the RSDs of 1.85％-3.28％.Their contents were the highest in G.elata Bl.form.glauca S.Chow cultivated in Zhaotong (collected in 2013),Lu'an (collected in 2013),Yichang (collected in 2013),Zhaotong (collected in 2014) and Yichang (collected in 2013),respectively,and the total content was the highest in G.elata Bl.form.glauca S.Chow cultivated in Yichang (collected in 2013).CONCLUSION This accurate,sensitive and reliable method can be used for the quality control of Gastrodiae Rhizoma.

Evaluation of saponins in Paris Polyphylla var.chinensis from twenty-one growing areas / 中成药

AIM To evaluate saponins in Paris Polyphylla Smith var.chinensis (Franch.) Hara from twentyone growing areas.METHODS HPLC was adopted in the content determination of pennogenins { pennogenin-3-O-β-D-glu (1→3) [α-L-rha (1→2)]-β-D-glu (PGGR),polyphyllinsⅥ,Ⅶ,H} and dioscins (gracillin,polyphyllins Ⅰ,Ⅱ,Ⅴ),then SPSS 22.0 software was used for principal component analysis and cluster analysis.RESULTS Pennogenin was the main kind of Paris Polyphylla var.chinensis saponins,and PGGR,polyphyllins Ⅰ,Ⅴ,Ⅶ,H were the main effective constituents of this medicinal material.The comprehension scores of seven growing areas were more than zero,which was in sequence of Bazhong ＞ Qingchuan ＞ Yanjin ＞ Tongjiang ＞ Nanchuan ＞ Chongzhou ＞ Hongya.Twenty-one batches of samples were divided into four types,which was similar to cluster analysis results.CONCLUSION Bazhong,Qingchuan,Chongzhou,Hongya,Tongjiang in Sichuan,Yanjin in Yunnan,and Nanchuan in Chongqing are suitable for cultivating Paris Polyphylla var.chinensis,which can guarantee the stability of saponins.

Quality evaluation of fifteen varieties of Epimedii Folium from different growing areas / 中成药

AIM To evaluate the quality of fifteen varieties of Epimedii Folium from different growing areas.METHODS UPLC-MS/MS was adopted in the content determination of icariin,icariside Ⅰ,icariside Ⅱ,epimedin A,epimedin B,epimedin C,sagittatoside A,sagittatoside B,2"-O-rhamnosylicariside Ⅱ and baohuoside Ⅱ.The analysis of 40％ ethanol extract of Epimedii Folium was performed on a 40 ℃ thermostatic Welch C18 column (50 mm × 2.1 mm,3 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner.Then principal component analysis was made.RESULTS Ten constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 97.6％-104.5％ with the RSDs of 1.37％-2.93％.In 46 batches of samples,epimedin C content was the highest,followed by epimedin A content,and icariside Ⅰ content was the lowest.The contents of various constituents showed obvious differences between small-flowered taxa and big-flowered taxa.CONCLUSION This sensitive and rapid method can provide a reference for the development and utilization of Epimedii Folium.

Quality evaluation of Linderae Radix from different growing areas / 中成药

AIM To evaluate the quality of Linderae Radix from different growing areas.METHODS Hot dipping method was applied to determining the extract content.HPLC was adopted in the content derermination of linderane,linderalactone and norisoboldine.Then SPSS19.0 software was used for principal component analysis.RESULTS The effect degrees of various index components were in sequence of extract ＞ norisoboldine ＞ linderalactone ＞ linderane.The accumulative contribution rate of the first two principal components (total content of four index components,extract content) reached 86.86％.The comprehensive score of Linderae Radix from Taizhou (Zhejiang) was the highest.CONCLUSION Taking Taizhou (Zhejiang) as the genuine producing area of Linderae Radix has a certain scientific basis.

Quality evaluation of fifteen varieties of Epimedii Folium from different growing areas / 中成药

AIM To evaluate the quality of fifteen varieties of Epimedii Folium from different growing areas.METHODS UPLC-MS/MS was adopted in the content determination of icariin,icariside Ⅰ,icariside Ⅱ,epimedin A,epimedin B,epimedin C,sagittatoside A,sagittatoside B,2"-O-rhamnosylicariside Ⅱ and baohuoside Ⅱ.The analysis of 40％ ethanol extract of Epimedii Folium was performed on a 40 ℃ thermostatic Welch C18 column (50 mm × 2.1 mm,3 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner.Then principal component analysis was made.RESULTS Ten constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 97.6％-104.5％ with the RSDs of 1.37％-2.93％.In 46 batches of samples,epimedin C content was the highest,followed by epimedin A content,and icariside Ⅰ content was the lowest.The contents of various constituents showed obvious differences between small-flowered taxa and big-flowered taxa.CONCLUSION This sensitive and rapid method can provide a reference for the development and utilization of Epimedii Folium.

Quality evaluation of Linderae Radix from different growing areas / 中成药

AIM To evaluate the quality of Linderae Radix from different growing areas.METHODS Hot dipping method was applied to determining the extract content.HPLC was adopted in the content derermination of linderane,linderalactone and norisoboldine.Then SPSS19.0 software was used for principal component analysis.RESULTS The effect degrees of various index components were in sequence of extract ＞ norisoboldine ＞ linderalactone ＞ linderane.The accumulative contribution rate of the first two principal components (total content of four index components,extract content) reached 86.86％.The comprehensive score of Linderae Radix from Taizhou (Zhejiang) was the highest.CONCLUSION Taking Taizhou (Zhejiang) as the genuine producing area of Linderae Radix has a certain scientific basis.