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Objective:To establish the HPLC fingerprint method for assessing the quality of Moutan Cortex, and to determine the contents of paeonol, paeoniflorin, gallic acid, hydroxyl-paeoniflorin and benzoyl-paeoniflorin of Moutan Cortex in different growth period. Methods:Diamonsil Plus C18 column (250 mm × 4.6 mm, 5 μm) was used with the mobile phase comprising acetonitrile-0.05% formic acid solution and the flow rate of 1.0 ml/min with gradient elution manner. The detected wavelength was 230 nm for paeoniflorin and benzoyl-paeoniflorin, 267 nm for gallic acid, 258 nm for hydroxyl-paeoniflorin and 274 nm for paeonol with temperature column of 25 ℃. Then putting chromatograms into Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica (2012A) to evaluate the similarity of Moutan Cortex in different growth period; then putting peak area data into SPSS software for cluster analysis and the clustering effect was determined. Results:The HPLC fingerprints established with this method has 23 shared peaks and 5 of them were identified, namely, paeonol, paeoniflorin, gallic acid, hydroxyl-paeoniflorin and benzoylpaeoniflorin. The similarity of Moutan Cortex in different years was between 0.850-0.991. This method has good linear relation ( r≥0.999 5), RSDs of precision, stability tests and reproducibility were lower than 1.6% ( n=6). Different growth periods of Moutan Cortex have obvious influence on the concentration of five compounds. Conclusion:This method is useful to evaluate and discriminate Moutan Cortex at different growth periods so as toprovide scientific reference on the harvest,industrialization and evaluation of Moutan Cortex.
ABSTRACT
Objective: To establish a HPLC fingerprint method for assessing the quality of Dendrobium huoshanense, in addition to determining concentrations of syringic acid, rutin, dendrophenol and naringenin in this crude drug. Methods: Agilent C18 column (250 mm × 4.6 mm, 5 μm) was utilized with the mobile phase comprising methanol-0.1% phosphoric acid with the flow rate of 1 mL/min in a gradient elution manner. The detection wavelength was set at 240 nm and the column temperature was 30 ℃. The resultant chromatograms were imported to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica (2012.1) to obtain retention time and peak area of samples. Similarity for 39 sets of samples were analyzed. The peak area data were processed by SPSS software for cluster analysis and the clustering effect was discussed. Results: The line relationship of this way was good (R > 0.999), with high precision regarding instrument used (RSD < 3.00%), the method showed good reproducibility (RSD < 3.00%), standard recovery was between 99.26% and 100.32% (RSD of 0.35%-1.67%). Different growth period and different planting patterns of D. huoshanense were distinct regarding the concentration of four compounds. Conclusion: The method is useful to evaluate and discriminate D. huoshanense at different growth period for the purpose of providing scientific reference on harvest, development and evaluation of D. huoshanense.
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Objective: To observe the effects of various factors on the antitussive effect of Siraitia grosvenorii. Methods: According to the sequential method (i.e., up and down method) for the principle of median effective dose, the antitussive effect of S. grosvenorii was observed and the median effective time (EDT50) of cough induced by NH3 aq. in mice was calculated. Results: S. grosvenorii from Hunan-Hengyang, Nanning-Tanluo, Yongfu-Longjiang, and Baishou have the antitussive effect, especially in Yongfu-Longjiang and Baishou (R>150%). The fruits obtained from cuttage seedlings and tissue culture seedlings have the antitussive effect, while the immature fruits have not (R130%) have the significant antitussive effect, and so first class fruit and ringing fruit do (R>130%). However secondary class fruit does not have the antitussive effect (R<130%). Conclusion: The factors, except the habitat, breeding technology, and whether drying or not, such as growth period and commercial specification may influence the antitussive effect of S. grosvenori.
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This study aimed to determine the relationships between the torque generating capacity of the lower extremity muscles and either running or jump performance in primary and junior high school boys. A total of 102 primary and junior high school boys participated in this study. Muscle thicknesses (MTs) of the knee extensors and plantar flexors were determined using ultrasonography. Muscle volumes (MVs) of the knee extensors and plantar flexors were estimated using MTs and limb lengths. The isometric joint torques (TQs) for knee extensors and ankle plantar flexors were measured using myometer. MV and TQ were divided by body mass (MV/BM and TQ/BM, respectively). Running velocity was measured using a non-motorized treadmill. The counter movement jump (CMJ) and squat jump (SJ) were performed on a matswitch system. The flight time was measured and used to calculate the heights of CMJ and SJ using the following equation; height (cm) = g × (flight time)<sup>2</sup> /8/10. As the result of multiple regression analysis, age, MV/BM and TQ/BM were selected as predictors of running velocity in the primary school boys, whereas TQ and lean body mass in junior high school boys. In the primary school boys, TQ/BM and body fat mass was selected as significant contributors for SJ and CMJ performances, whereas, in the junior high school boys, TQ and the percent of body fat for SJ performance and MV/BM and TQ for CMJ performance. Thus, the present results indicate that the relationships between torque generating capacity of the lower extremity muscles and either running or jump performance differ between primary and junior high school boys. It may be assumed that, for running and jump performances, muscle mass and strength become determinant factors in junior high school boys, whereas their values relative to body mass in primary school boys.
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The purpose of this study was to examine factors related to the occurrence of enthesis pain by long-term longitudinal investigation and measurement in junior high school soccer players, while intervening for its prevention. The subjects were 107 male junior high school soccer club members. The presence or absence of enthesis pain, the height growth velocity, muscle tightness, and alignment were longitudinally investigated, and stretching was instructed periodically. The time of enthesis pain occurrence was consistent with phase II of the height growth velocity curve in many cases. On comparison between before and after the appearance/disappearance of enthesis pain, no association was noted between the muscle tightness and occurrence of enthesis pain. But, on comparison of changes with time in muscle tightness, the tightness of the right quadriceps significantly increased, being useful for the prediction of enthesis pain. A significant increase in the Q-angle was also noted when enthesis pain appeared. Furthermore, the tightness of the bilateral iliopsoas, hamstrings, hip adductors, and gastrocnemius had significantly improved on the final measurement, showing that instruction in stretching was effective. This study showed the necessity of the long-term longitudinal evaluation of muscle tightness, suggesting that there is a possibility of intervention for prevention, as well as physical therapy for sporting injuries in the growth period.
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The purpose of this study was to examine the bone stiffness of healthy girls during their growth period and factors affecting on such stiffness. The subjects were 302 secondary school girls in Tokyo. Parameters examined included the body characteristics (standing height, body weight, bone stiffness measured by ultrasound, muscle thickness measured with the ultrasound B-mode system), extension power of the lower limb (containing the knee and hip joints), and a questionnaire about the daily intake of milk, kinds of meals and number of years from menophania. These examinations were carried out in June 1997.<BR>Results were summarized as follows : 1) From the observation of bone stiffness in secondary school girls, it appears that bone stiffness increases during the junior high school period. In other words, the bone stiffness of the subjects had almost reached on adult level by high school. 2) A significant positive correlation was recognized between chronological age and bone stiffness (r=0.365, p<0.05) . A positive correlation also existed between the years from menophania and bone stiffness (r=0.477, p<0.05) . These coefficients showed that the years from menophania correlate with bone stiffness more closely in comparison with chronological age. 3) In the period when short comparatively years from menophania, body characteristics, which were the index of maturity, correlated to bone stiffness. However, muscle thickness/power, which was related to exercise habit, became the major parameter correlating with bone stiffness. These results suggest that factors affecting bone stiffness should differ according to the growth period. 4) Comparison of different athletic clubs showed that the bone stiffness of volleyball players was higher than that of control. These data suggest that physical education class, which was given 3 times a week, is not enough for total body development in both quality and quantity. In other words, physical education should be better matched with total physical development, including the growth of healthy bones.
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The method of treatment in skeletal Class III malocclusion must be chosen according to an etiology and timing of the treatment. Maxillary protraction has been used as an effective treatment method in growing children with maxillary deficiency. The efficacy of maxillary protraction has been viewed as a result of downward-backward displacement of mandible and compensatory dental displacement during the treatment rather than forward-downward growth of maxilla itself. In this study, 104 subjects treated with maxillary protraction, and 19 males and 21 females with known annual growth amount have been chosen longitudinally as treated group and normal group, respectively. And changes in position of maxilla, mandible and dentition have been comparatively analyzed on the lateral cephalometric radiographs by age. The results were as follows: 1. Treated group showed more forward movement of maxilla compare to the normal group and the mandible displaced backward compare to the normal group. 2. Downward movement of maxilla in treated group was similar to that of normal group with statistical signigicance in female 12 year old group and downward movement of mandible in treated group was similar to that of normal group. 3. In treated group, maxillary central incisor moved more forward than the normal group with statistical significance in male 8, 10 year-old groups and female 8, 9, 10 year-old groups. In treated group, downward movement of maxillary central incisor was similar to that of the normal group with statistical significance in male and female 7, 8 year-old groups. Considering the above results and the duration of the treatment, the forward movement of maxilla due to maxillary protraction was effective compared to normal growth amount of the normal group.