ABSTRACT
Haematopoiesis is a complex process in which the regulatory mechanisms of several implicated transcription factors remain uncertain. Drosophila melanogaster is an excellent model to resolve the unanswered questions about the blood cell development. This study describes the role of Beadex, a Drosophila homologue of LIM domain only 2 (LMO2), in haematopoiesis. Mutants of Beadex were analysed for blood cell abnormalities. Crystal cells, a subset of haemocytes, were significantly more in Beadex hypermorphic flies. Similarly, Beadex misexpression in prohemocytes altered the crystal cell numbers. Stage-specific misexpression analyses demonstrated that Beadex functions after the prohemocytes enter the crystal cell lineage. We also discovered that Pannier–U-shaped complex is a negative regulator of the crystal cell differentiation and is possibly negatively regulated by Beadex through its interaction with Pannier. We, therefore, suggest the mechanism of two novel regulators of crystal cell specification—Beadex and Pannier—during Drosophila haematopoiesis.
ABSTRACT
This study assessed the impact of the electrochemically activated aqueous solutions (ECA AS) on the growth and formationof the biofilm of lactic acid bacteria (LAB). The effectiveness of biocides produced by the unipolar electrochemicalactivation of aqueous solutions of sodium chloride was studied. It was found that sequential treatment of the biofilm withcatholyte and anolyte (fractions ECA AS) lead to the most pronounced decrease in the growth rate and density of bacteria.The results obtained demonstrate antibacterial efficacy and the possibility of using ECA AS for the prevention anddisinfection of aquatic systems, for example, at the enterprises of the agro-industry and the food industry
ABSTRACT
Patients with acute myeloid leukaemia (AML) who have FMS-like tyrosine kinase 3 (FLT3) mutations are a concern for haematologists. Many studies indicate that these patients have poor prognosis. Due to the dearth of research in this area, the role of allogeneic transplantation as a therapy is still controversial. But the available limited data suggest that transplantation in first remission is possibly the first choice. As FTL3 mutations in AML result in poor patient outcomes, different FLT3 inhibitors are used as specific targeted therapy.
ABSTRACT
Malaria is a major health problem in India. In cases of fever with thrombocytopenia, malaria should be on top of the differential diagnosis. This was a study of admitted patients in medical wards of Bhaskara Medical Colleges, Hyderabad who were diagnosed with malaria and they were assessed for thrombocytopenia using automated quantitative D3 analyser. The data were analysed using SPSS version 16.0. Viral haemorrhagic fevers can also be presented as fever with high haematocrit and leucopenia along with thrombocytopenia. In our study, the commonest cause of thrombocytopenia in malaria was vivax malaria.
ABSTRACT
El ciclo celular constituye el conjunto de eventos que determinan la actividad metabólica de toda célula así como su división y la generación de células hijas. Este proceso está involucrado en todos los procesos celulares tales como proliferación y diferenciación. Se ha demostrado que diversos elementos moleculares participan en su regulación y que alteraciones en este proceso pueden conducir a trastornos fisiológicos e incluso a la muerte del organismo. En los mamíferos, uno de los tejidos con mayor recambio celular es el hematopoyético, por lo que se requiere un control muy estricto del ciclo celular, principalmente a nivel de células troncales y progenitoras para la óptima producción de células sanguíneas. En este artículo presentamos un panorama general de los principales mecanismos y factores involucrados en la regulación del ciclo celular, poniendo particular énfasis en su papel en la biología de las células hematopoyéticas primitivas.
The cell cycle comprises a group of biological events that determine the metabolic activity of any cell, as well as its division and the generation of new daughter cells. It is involved in every cellular process, such asproliferation and differentiation. It has been shown that several molecular elements regulate the cell cycle, and that alterations in any of its phases and/or regulators could give rise to physiologic deficiencies, and even death. In mammals, the haematopoietic tissue is one of the most active in terms of cell replacement; thus, a tight cell cycle control, particularly at the level of stem and progenitors cells is crucial for optimal blood cell production. In the present article, we give a comprehensive overview of the major mechanisms and regulatory, molecules that participate in the cell cycle of mammalian cells, with particular emphasis on its role in the biology of primitive cells of the hematopoietic system.