ABSTRACT
Objective T o establish an autom ation system for detection of alcohol content in blood. Meth-ods T he determ ination w as perform ed by autom ated w orkstation of extraction-headspace gas chrom atog-raphy (H S-G C ). T he blood collection w ith negative pressure, sealing tim e of headspace bottle and sam-ple needle w ere checked and optim ized in the abstraction of autom ation system . T he autom atic sam pling w as com pared w ith the m anual sam pling. Results T he quantitative data obtained by the autom ated w ork-station of extraction-H S-G C for alcohol w as stable. T he relative differences of tw o parallel sam ples w ere less than 5%. T he autom ated extraction w as superior to the m anual extraction. A good linear rela-tionship w as obtained at the alcohol concentration range of 0.1-3.0 m g/m L (r≥0.999) w ith good re-peatability. Conclusion T he m ethod is sim ple and quick, w ith m ore standard experim ent process and ac-curate experim ental data. It elim inates the error from the experim enter and has good repeatability, w hich can be applied to the qualitative and quantitative detections of alcohol in blood.
ABSTRACT
Objective To discuss blood collection tubes with different additives and their effects on the testing results of alcohol concentration in blood sam ples. Methods Blood sam ples from 10 volunteers were collected 2 hours after drinking with seven different types of disposable vacuum blood collection tubes, including ordinary tube without anticoagulant, coagulant tube, separating gel-coagulant tube, sodi-um citrate (1∶4) tube, sodium citrate (1∶9) tube, sodium citrate (9∶1) tube and EDTA-K2 tube. The al-cohol concentrations in these blood sam ples were analyzed by headspace gas chrom atography. Results The concentration testing results of the sam e blood sam ples in different types of tubes were different from one to another. The sequence was as follows:separating gel-coagulant tube>coagulant tube>ordi-nary tube without anticoagulant>EDTA-K2 tube>sodium citrate (1∶9) tube>sodium citrate (1∶4) tube, whereas the results of the sam e blood sam ple in sodium citrate (1∶9) tube and sodium citrate (9∶1) tube showed no obvious difference. Conclusion It is better to collect a suspicious drunk driver’s blood sam-ple using a disposable vacuum blood collection tube, with the EDTA-K2 tube being preferred.