ABSTRACT
Objective To investigate the interference role that two kinds of adipokines,adiponectin and leptin,exert to hepatocellular apoptosis in mice with acute liver injury induced by Concanavalin A(ConA).MethodsTwenty-six male BALB/c mice were divided into four groups randomly:ConA group(8 mice),adiponectin group(5 mice),leptin group(5 mice) and control group(8 mice).Mice were sacrificed 8 hours after ConA-treatment for observing hepatic injury and hepatocellular apoptosis.Results Comparison to ConA group,the mice in adiponectin group showed less hepatic injury and lower ALT level and less hepatocellular apoptosis(P
ABSTRACT
Objective To study the relationship between hepatocellular apoptosis and glycogen contents during hepatic cold preservation reperfusion and its mechanism.Methods Based on the model of four groups of rabbit livers with different hepatocellular glycogen contents, hepatocellular apoptosis and bax gene expression were observed during hepatic cold preservation reperfusion.Results Apoptotic hepatocytes were obviously found in 60 minute reperfusing livers subsequent to 9 hour cold storage, and there was significant difference in the numbers of apoptotic hepatocytes among all the groups. In the same time, there was the close relationship between the levels of bax gene expression and the glycogen contents of hepatocytes.Conclusion Intracellular abundant glycogen may significantly depress the hepatocellular apoptosis during hepatic cold preservation reperfusion by decreasing hepatocellular bax gene expression.