ABSTRACT
In recent years, gene engineering is developing rapidly and many recombinant proteins have been expressed. The use of plant bioreactor to express specific pharmaceutical proteins provides a new way for the prevention and treatment of some important diseases in human beings. Nowadays, chloroplast genetic transformation and expression system has become a research hotspot in plant bioreactor. Higher plant chloroplasts have unique advantages in the expression of recombinant proteins due to their special structures and inherited characteristics: such as high expression, site-specific integration, and the maternal inheritance characteristics of exogenous genes. The maternal inheritance of chloroplast is helpful for biological safety of transgene escaping by pollens. Many important pharmaceutical proteins have been successfully expressed in plant chloroplasts. As a chloroplast transformation model of higher plants, tobacco has made significant progress in the expression of pharmaceutical proteins, such as vaccine antigens, antibodies, and other important recombinant proteins. Chloroplast genetic transformation in higher plants also provides new techniques and methods for the study of chloroplast gene expression and regulation mechanisms. In order to provide a new idea for the development of chloroplast expression platform and the expression of important pharmaceutical proteins, this review outlined the progress of chloroplast genetic transformation system in higher plants, including the chloroplast transformation principle, vector construction, expression of recombinant proteins and important pharmaceutical proteins, and the effects of recombinant proteins expression on plant metabolism and traits.
ABSTRACT
Background: Because of its strong specificity and high accuracy, real-time quantitative PCR (RT-qPCR) has been a widely used method to study the expression of genes responsive to stress. It is crucial to have a suitable set of reference genes to normalize target gene expression in peanut under different conditions using RT-qPCR. In this study, 11 candidate reference genes were selected and examined under abiotic stresses (drought, salt, heavy metal, and low temperature) and hormone (SA and ABA) conditions as well as across different organ types. Three statistical algorithms (geNorm, NormFinder and BestKeeper) were used to evaluate the expression stabilities of reference genes, and the comprehensive rankings of gene stability were generated. Results: The results indicated that ELF1B and YLS8 were the most stable reference genes under PEG-simulated drought treatment. For high-salt treatment using NaCl, YLS8 and GAPDH were the most stable genes. Under CdCl2 treatment, UBI1 and YLS8 were suitable as stable reference genes. UBI1, ADH3, and ACTIN11 were sufficient for gene expression normalization in low-temperature experiment. All the 11 candidate reference genes showed relatively high stability under hormone treatments. For organs subset, UBI1, GAPDH, and ELF1B showed the maximum stability. UBI1 and ADH3 were the top two genes that could be used reliably in all the stress conditions assessed. Furthermore, the necessity of the reference genes screened was further confirmed by the expression pattern of AnnAhs. Conclusions: The results perfect the selection of stable reference genes for future gene expression studies in peanut and provide a list of reference genes that may be used in the future.
Subject(s)
Arachis/genetics , Genes, Plant , Real-Time Polymerase Chain Reaction/methods , Plant Growth Regulators , Reference Standards , Selection, Genetic , Stress, Physiological , Gene Amplification , Cold Temperature , Gene Expression Profiling , DroughtsABSTRACT
Objective: The research is to provide the basis for effective protection and wise utilization of the plants resources inTea Mountain National Ecological Demonstration Area of Shandong Province. Methods: The general investigation on plant species inTea Mountain was conducted. The research scope covered plant species, their distribution in wild, community structure and flora.Results: A total of 352 higher plants, belonging to 250 genera and 90 families, were recorded. Four species were listed as nationalsecond-grade protected plants. Regional vegetation is Pinus thunbergii plantation and Secondary forest. The main forest vegetation isPinus thunbergii community. Pantropic types and N. Tem types are dominant types. The latitude and "the islands-effect" should be themajor-effect factor of this pattern. Because of intense artificial active disturbance, primary vegetation types in this region have sufferedserious destruction, especially the rare and precious plants that are at high risk of dying out. Conclusions: This regional communitystructure is simple. The shrub layer and the liana layer are undeveloped. The syngenesis time is short.
ABSTRACT
Objective: The research is to provide the basis for effective protection and wise utilization of the plants resources inTea Mountain National Ecological Demonstration Area of Shandong Province. Methods: The general investigation on plant species inTea Mountain was conducted. The research scope covered plant species, their distribution in wild, community structure and flora.Results: A total of 352 higher plants, belonging to 250 genera and 90 families, were recorded. Four species were listed as nationalsecond-grade protected plants. Regional vegetation is Pinus thunbergii plantation and Secondary forest. The main forest vegetation isPinus thunbergii community. Pantropic types and N. Tem types are dominant types. The latitude and "the islands-effect" should be themajor-effect factor of this pattern. Because of intense artificial active disturbance, primary vegetation types in this region have sufferedserious destruction, especially the rare and precious plants that are at high risk of dying out. Conclusions: This regional communitystructure is simple. The shrub layer and the liana layer are undeveloped. The syngenesis time is short.
ABSTRACT
Objective: The research is to provide the basis for effective protection and wise utilization of the plants resources inTea Mountain National Ecological Demonstration Area of Shandong Province. Methods: The general investigation on plant species inTea Mountain was conducted. The research scope covered plant species, their distribution in wild, community structure and flora.Results: A total of 352 higher plants, belonging to 250 genera and 90 families, were recorded. Four species were listed as nationalsecond-grade protected plants. Regional vegetation is Pinus thunbergii plantation and Secondary forest. The main forest vegetation isPinus thunbergii community. Pantropic types and N. Tem types are dominant types. The latitude and the islands-effect should be themajor-effect factor of this pattern. Because of intense artificial active disturbance, primary vegetation types in this region have sufferedserious destruction, especially the rare and precious plants that are at high risk of dying out. Conclusions: This regional communitystructure is simple. The shrub layer and the liana layer are undeveloped. The syngenesis time is short.