ABSTRACT
Objective To study the protective effects of hydroxysafflor yellow A (HSYA) against the oxidative damage caused by β-mercaptoethanol (BME) during neural differentiation of mesenchymal stem cells (MSCs) in vitro. Methods When the confluence reached 50%-60%, 4th passage MSCs were divided into three groups to culture. G1: normal group which was cultured using basic medium (DMEM containing 10% FBS all the time); G2: unprotected group which was continuously cultured using basic medium for 24 h, and then cultured using pre-induction medium (DMEM containing 10% FBS and 1 mmol/L BME); G3: protected group which was firstly cultured using protective medium (DMEM containing 10% FBS and 160 mg/L HSYA) for 24 h, and then cultured using pre-induction medium for 24 h. After these treatments as above, cell viability, relative levels of SOD/GSH and apoptosis rate were respectively detected. The expression of Bcl and Bax was examined by Western blotting. After HSYA protection and BME pre-induction, neural induction was performed. The expression of NSE and MAP-2 was respectively analyzed on cellular and molecular levels. Results Compared with unprotected group, 160 mg/L HSYA could obviously improve cells viability, maintain high level of SOD and GSH in MSCs, reduce apoptosis rate and improve the ratio of Bcl/Bax. After protection with 160 mg/L HSYA, the survival time of neuron-like cells could be extended. Immunocytochemical staining showed that after 10 h of neural induction, the differentiated neuron-like cells in protected group were still in a good state, and the mRNA levels of NSE and MAP-2 were increased during the induction course checked. Conclusion HSYA could improve the resistance of cells to the oxidative damage caused by BME.
ABSTRACT
Objective To investigate the effect of hydroxyl safflor yellow A on mediating blood lipid and to explore its primary mechanism.Methods Fifty KM mice were divided into five groups randomly:control group(A),hyperlipidemia model group(B),high-dose group(C),mid-dose group (D) and low-dose group(E).C,D and E group were injected by hydroxy safflor yellow A with 10,40 and 70 mg.kg-1 day-1respectively,while A and B group were both injected by saline with 0.4 ml day-1,the administrations were kept on three days.The levels of serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C) and malondialdehyde (MDA) were assayed,simultaneously the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed after 18 hours.Results Compared with the control group,the serum TC (4.09+0.2) mmol/L,TG (0.96±0.15) mmol/L,LDL-C (5.87±0.17) mmol/L,HDL-C (0.83±0.21) mmol/L,MDA (8.26+1.05) nmol/ml,and the activities of SOD (330.18 ± 11.45 ) U/ml,GSH-Px (1023.54±25.34) U/ml of model group injected high doses of hydroxysafflor yellow A were statistically significant.Conclusion Hydroxy safflor yellow A had the function of mediating blood lipid.Anti-oxidation could be the mechanism.
ABSTRACT
Objective To establish content determination of hydroxyl safflower yellow A in Biyangqing.Methods Hhgh-performance liquid chromatography(HPLC)was used in the determination.A C18 column was used for the separation flow rate Was set at 1.0mL/min,the temperature of the column was set at 30℃,and wavelength of diction was set at 403 nm.with 100.08%average recovery and 0.98%RSD.Conclusion This detrmination method is specific and reproducible and can be used to control the quality of Biyangqing.