ABSTRACT
Objective To investigate the effect of hyperbaric oxygen preconditioning on syngeneic kidney transplantation in rats and to explore the potential mechanism. Methods Forty male SD rats were randomly divid-ed into control group(Sham group),kidney transplantation group(KTx group)and hyperbaric oxygen precondi-tioning group(HBO+KTx group). Kidney transplantation was performed 24 h after hyperbaric preconditioning. Rats were sacrificed 24 h after the transplantation.Serum creatinine(Scr)and blood urea nitrogen(BUN)were de-tected.The level of superoxide dismutase(SOD),malondialdehyde(MDA)and heme oxygenase-1(HO-1)in the graft was examined. Kidney pathological examination was performed after renal tissue fixed. Results Compared with the KTx group,HBO + KTx group showed lower Scr and BUN level. The MDA level was decreased and the SOD activity was increased significantly after hyperbaric oxygen preconditioning in HBO+KTx group.Renal tubu-lar necrosis was significantly reduced in HBO+KTx group.HO-1 level in HBO+KTx group was significantly high-er than that of KTx group. Conclusion Hyperbaric oxygen preconditioning can reduce lipid peroxidation,en-hance antioxidant enzyme activity and improve the level of HO-1 in graft tissue,thus attenuating kidney injury.
ABSTRACT
Objective To explore the effects of hyperbaric oxygen preconditioning on ischemia-reperfusion inflammatory reaction of rats after skin flap transplantation.Methods Fifty-six Sprague-Dawley rats were randomly divided into 3 groups:a sham ischemia-reperfusion (SH) group,an ischemia-reperfusion (IR) group and a hyperbaric oxygen reconditioning (HBO) group.Both IR group and HBO group were further divided into 3 subgroups,respectively,according to the time points of serum sampling for test post establishment of the IR model of the abdominal pedicle skin flap transplantation.The IR model of the abdominal pedicle skin flap transplantation was established in all the animals except those in the SH group,with those in the HBO group were preconditioned with HBO twice daily for 3 days before the operation.The blood was sampled at 1,3 and 5 day post-operation to test the level of IL-23 using enzyme-linked immunosorbeut assay (ELISA).The survival skin flaps were sampled from all the animals at 3 and 5 days after the operation for histological observation and evaluation.Results The average IL-23 level of HBO 3 d subgroup (17.80 ± 14.78) was significantly lower than that of the IR 3 d subgroup (38.91 ± 12.26).The average histological scores of the IR 3 d and 5 d subgroups,as well as HBO 3 d and 5 d subgroups were (2.66 ±0.44)and (3.2 ±0.53),(1.85 ±0.31) and (2.29 ±0.32),significantly higher than SH group (0.38 ±0.10).Moreover,the average histological score of the HBO 3 d and 5 d subgroups was significantly lower than IR 3 d and 5 d subgroups respectively.Conclusion Hyperbaric oxygen preconditioning can relieve the ischemia-reperfusion inflammatory reaction through reducing the serum level of IL-23 in rats after skin flap transplantation.