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Population aging has become a major feature of the demographic structure of today's society， and anti-aging is currently one of the major areas and hot spots of medical research. Kidney deficiency causes aging is the basic theory of aging in TCM， and kidney tonics have a long history of improving physiological functions and significantly alleviating aging-related diseases. However， the formulation of kidney tonics is complicated and the mechanism of action is still unclear. It is difficult to precisely analyze the targets of disease action and metabolic pathways. The kidney tonic herb Epimedium has the effect of tonifying the kidney and augmenting yang， and is not only used to treat diseases related to kidney deficiency and aging， but also has unique advantages in the treatment of mental and emotional diseases. Modern pharmacological studies have proved that Icarrin， a monomeric component of Epimedium， can produce a wide range of biological functions on cardiovascular system， exercise system， reproductive system， and nervous system， with strong antioxidant， free radical scavenging， and repair of mitochondrial DNA damage， which can anti-aging， anti-depression， and improve immunity， etc. In this paper， we searched databases such as CNKI， Pubmed， Wanfang database， Wipu Chinese journal service platform， and web of science to review the research on Icarrin， a monomeric ingredient that has both anti-aging and anti-depression effects， in order to provide a basis for further research on anti-depression and anti-aging in Chinese medicine.
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Objective@#To investigate the effect of icariin (ICA) on the bisphenol A (BPA)-enhanced proliferation function of thyroid carcinoma cell B-CPAP and underlying mechanism.@*Methods@#The proliferation of Gastric B-CPAP cell line was evaluated by cell counting kit-8 (CCK-8). Apoptosis and ROS expression in B-CPAP cells were detected by flow cytometry. The expression of superoxide dismutase (SOD) and malondialdehyde (MDA) in B-CPAP cells were measured by individual assay kits. The expressions of Bcl-2 and γ-HA2X were detected by Western blot. SPSS 18.0 software was used to analyze the data.@*Results@#B-CPAP cell activity was promoted by treatment with 3×10-7mol/L BPA for 48 h, with significant difference in absorbance between BPA and control groups (1.089±0.053 vs 0.935±0.010, P<0.05). The cell activities of BPA+ ICA25, BPA+ ICA50, BPA+ ICA100 and BPA+ ICA200 groups was 0.780±0.036, 1.007±0.050, 0.958±0.033 and 0.625±0.064, respectively (all P<0.01). The proliferation of B-CPAP cells treated with BPA for 72 hours showed a similar trend to 48 hours. There was no significant difference between all treatment groups in 24 hours. The apoptosis rate was (19.272±0.186)% in BPA-treated cells, and was (22.412±0.238)% in control cells (P<0.05). The apoptosis rates of BPA+ ICA50 and BPA+ ICA200 groups were (23.688±0.412)% and (30.270±0.696)%, respectively (P<0.01). The intracellular accumulation of ROS in BPA, BPA+ ICA50, and BPA+ ICA200 groups were 806±21, 1 772±37, 2 041±16, respectively (P<0.01). The expressions of anti-apoptotic protein Bcl-2 in control, BPA, BPA+ ICA50, BPA+ ICA200 groups were 7 120±151, 9 801±286, 5 902±171 and 4 203±216, respectively (P<0.01).@*Conclusion@#BPA can promote the proliferation of thyroid carcinoma B-CPAP cells and decrease the apoptosis of cells, and this effect can be inhibited by ICA. The possible mechanism is to induce high expression of intracellular ROS and inhibit the expression of antioxidase system, leading to cell oxidative damage, thereby inducing apoptosis.
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Objective To investigate the effects of icariin, a major active component of traditional Chinese herb Epimedium on schizo-phrenia animal model. Methods MK-801, a N-methyl-D-aspartate (NMDA) receptor antagonist, was intraperitoneally injected into mice to establish a schizophrenia animal model. The open field behavior test infrared detection system was used to measure the mobility of mice in order to find the best optimal dose. 40 mice were randomly divided in to control group, model group, risperidone group and icariin group with 10 mice in each group. The risperidone group and the icariin group were given 0.1 mg/kg risperidone and 50 mg/kg icariin respectively. All groups were injected with 0.6 mg/kg MK-801 0.1 ml intraperitoneally, except the control group. The total distance and the central dis-tance within 210 minutes were tested. Results The total distance and the central distance were significantly longer in the model group than in the control group (P0.05). Conclusion Icariin may improve the positive and negative symptoms of schizophrenia mice, which may be beneficial to schizo-phrenia therapy.
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Objective To observe the effect of icarrin on proliferation and differentiation of rat osteoblasts cultured in vitro as well as the expressions of bone morphogenetic protein-2 (BMP-2) and Osterix in rat osteoblast.Methods Calvarial osteoblasts were obtained from newborn (＜ 24 h) SD rats by trypsin-collagenase digestion.The culture medium with different icarrin concentrations and the second generation osteoblasts were mixed.The mRNA expressions of BMP-2,Osterix,ALP,and Col Ⅰ were detected by semiquantative RT-PCR.Then the second generation osteoblasts were cultured in the medium containing icariin (10 ng/ml) with or without BMP-2 antibody.After48 h,RT-PCR was used to estimate the mRNA expression of Osterix.Results In comparison with the control group,the mRNA expressions of BMP-2,Osterix,ALP,Col Ⅰ were increased in a dose-dependent manner (P＜0.05) with a maximal effect at the concentration of 10 ng/ml.The mRNA expression of Osterix treated with the mixture of icarrin and BMP-2 antibody was significant decreased(P＜0.05),and compared with the group of icarrin,however,the difference between the BMP-2 group and the mixture group was not statistically significant (P ＞ 0.05).Conclusions Icarrin stimulates proliferation and differentiation of cultured osteoblast in vitro by increasing the mRNA expression of ALP and Col Ⅰ.Icarrin could stimulate Osterix gene expression by enchancing BMP-2 gene expression,finally,it could induce bone formation and prevent and/or treat osteoporosis.
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Objective To study on purication of stilbene glycoside and icarrin with macroporous resin of radix polygoni multiflori praeparata cum succo glycines sotae and herba epimedii of Fufang Bushenshengjing Capsule. Methods To select the best type of resin from NKA-9, AB-8, D101, X-5 and DM130, to observe the factor of the concentration of water-extraction, the volume of resin, pH of water-extraction, the concentration and volume of ethanol. Results Using AB-8 resin, the concentration of water-extraction is 0.3 g/mL, the volume of resin (mL) to the mass of medicinal materials is 2, pH of water-extraction need't change, eluant is 70% ethanol and its volume (mL) is 5 times of the mass of medicinal materials (g). Conclusion This research can provide reference for the extraction of active component from Fufang Bushenshengjing capsule in industrial production.
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Objective To optimize the extraction process for icariin from Herba Epimedii. Methods The content of icariin in the extracts obtained by different extraction methods was determined with HPLC. The effect of solvents, boiling time, extracting time and temperature, and the size of the medicinal powder was observed. Results Different extraction methods had great influence on the extraction rate of icariin. Conclusion This research can provide evidence for the extraction of active component from Herba Epimedii in industrial production.
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AIM: To establish the method for quality control of Guli Granules(Herba Epimedii,Radix Puerariae Lobatae,Rhizoma Curcumae Longae,Radix Achyranthis Bidentatae,Fructus Psoraleae,etc.). METHODS: Radix Puerariae,Rhizoma Curcumae Longae,Radix Achyranthis Bidentatae and Fructus Psoraleae in this medicine were identified by TLC.The icarrin content in this medicine was determined by HPLC.The acetonitril-water(28∶72) solution was used as the mobile phase,the column was C_(18),the flow rate was 1.0 mL?min~(-1).The detection wavelength was at 270 nm. RESULTS: These herbs could be identified by TLC.For Icarrin,the linear range was from 17 to 85 ?g?mL~(-1),and the average recovery was 98.32% with RSD of 1.67%. CONCLUSION: The method is simple,reliable,accurate and can be applied to the quality control of the preparation.
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AIM: To establish the method for quality control of Yijingbushen Granules(Herba Epimedii, Semen Cuscutae, Radix Paeoniae Alba, Rhizoma Curcumae, etc.). METHODS:Semen Cuscutae, Radix Paeoniae Alba and Rhizoma Curcumae in this medicine were identified by TLC; The content of icarrin in this medicine was determined by HPLC;The acetonitrile-2%acetum(30∶70) solution was used as the mobile phase. The flow rate was 1.0mL?min -1. The detection wavelength was set at 270nm. RESULTS:Semen Cuscutae, Radix Paeoniae Alba and Rhizoma Curcumae, could be identified by TLC on lichrospherC 18 column. For Icarrin, the linear range was from 4.32 to 21.60?g, and the average recovery was 98.69% with RSD of 1.13%. CONCLUSION:The method is simple, reliable, accurate and can be applied to the quality control of the preparation.
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AIM To investigate the effect of Icarrin on penile erection and cyclic GMP levels in rabbit corpus cavernosum(RCC). METHODS Effects of various concentration of Icariin on cGMP level in RCC were measured by 125 I radioimmunoassay procedure with Sildenafil and papaverine as the positive controls RESULTS In the presence of SNP, Icariin increased cGMP concentration in rabbit corpus cavernosum in a dose dependent manner ( P
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In this paper we report im-munopharmacologic effects of Epimedium Polysaccharide (EPS) and Icarrin on mouse thy-mus. It was found that EPS or Icarrin administered subcutaneously in mouse had following effects: (1)EPS enhanced the proliferation and IL-2 production of thymocytes, and decreased the L3T4 and Lyt2 cell numbers and responses to Con A stimulation of the thymus; (2) Icarrinincreased response to ConA stimulation to mouse thymus cell. These results suggest that both EPS and Icarrin have immunes stimulating effect on thymus. EPS possibly promoted the migration of thymocytes from the thymus to peripheral organs.