Advances in methodologies for preparation and analysis of new biochromatic stationary phase / 药学实践杂志

Biochromatography is a new chromatographic technology with great development potential. It has been widely used in drug screening and biomolecular interaction analysis. The core of this technology is the chromatographic stationary phase of biomolecules. Nowadays, it mainly develops cell membrane chromatography, artificial biomimetic membrane chromatography and the various immobilization strategies to directly immobilizes proteins on the stationary phase carrier. This paper reviews the research progress of new biochromatographic stationary phase and the application of biochromatographic analysis based on new stationary phase. And, the applications of biochromatographic stationary phase and micro biochromatographic analysis system based on monolithic column are prospected.

A Pilot Evaluation of Cast Immobilization of Intact Upper Extremity: A New Therapeutic Approach for Spastic Hemiplegic Cerebral Palsy

OBJECTIVE: To investigate the functional facilitation effect on an affected side of spastic hemiplegic cerebral palsy by immobilizing the intact side with a cast. METHOD: Five hemiplegic cerebral palsies, who had been treated by general rehabilitation therapy during previous 6 months and showed no functional improvement were studied. Their intact arms were immobilized by scotch short arm casts for 6 weeks. And, the therapeatic effects of this method were evaluated by the Box and Block Test of manual dexterity, Erhardt Developmental Prehension Assesment and Brain Perfusion SPECT. RESULTS: 1. Box and Block test: Before the cast immobilization method, hemiplegic cerebral palsies could transfer a mean of 5.4+/-3.36 cubic rods from one to the other side, and after the treatment a mean of 8.0+/-1.41 cubic rods with the affected arm. 2. Erhardt Developmental Prehension Assesment: Before the treatment, the grasp motion of round rod, cubic rod, and button suited in a mean of 5.1+/-3.0 months, and after the treatment, suited in a mean of 8.2+/-0.66 months. 3. Brain Perfusion SPECTs did not change before and after the cast immobilization method. 4. There was no adverse effect resulting from the cast immobilization method on an intact side. CONCLUSION: We concluded that the cast immobilization method on an intact side could be a new beneficial rehabilitation method for the treatment of spastic hemiplegic cerebral palsies.