ABSTRACT
Objective To investigate the preparation of the internal quality controls for urinary retinal binding protein(RBP)de-tected by the latex enhanced immunoturbidimetry assay and its performance evaluation.Methods The urine specimens from the pa-tients with nephropathy and healthy people with physical examination were collected,centrifuged by 3 500 r/min for removing sedi-ment,urinary RBP levels were adjusted to the low value for healthy population and the high value for the patients with nephropa-thy;then conducted the anticorrosion,packed and stored at -20 ℃.By using the urinary RBP commodity kits,the latex enhanced immunoturbidimetry was adopted to detect urinary RBP,the intra-batch and inter-batch precisions were detected respectively.Then the high and low values of self-prepared controls were detected once per day for consecutive 5 months.The data were processed by using the SPSS13.0 software.Then the performance of self-prepared urinary RBP quality control was evaluated.Results The intra-batch imprecision of 2 levels of urinary RBP controls was less than 5% and the inter-batch imprecision was less than 6%;the stable period by storage at -20 ℃ was at least 5 months,the imprecision had no statistical difference within 5 months(P >0.05).Conclu-sion The high and low levels of self-prepared urinary RBP quality controls can meet the operating requirements of internal quality controls.
ABSTRACT
Objective To study the levels of serum apolipoproteins in the middle aged in Qingdao city.Methods 2 287 subjects(male 1 631,female 656) aged 35～59(44.70?5.99) yrs in Qingdao city were randomly recruited.Their serum Apolipoprotein B(ApoB) and Apolipoprotein E(ApoE) were measured by immunoturbidimetry assay.Results The results of ApoAI,ApoB,ApoE and ApoB/AI were(1.36?0.22)g/L,(0.99?0.26)g/L,(4.89?1.83)mg/dl and 0.75?0.23,respectively.The abnormal ratios of Apo AI,Apo B,Apo E and ApoB/AI were 12.77%,29.03%,28.82% and 6.56%,respectively.With the partial correlation analysis adjusted by age,sex and BMI,the cardiovascular risk factors score were positively related with the level of Apo AI,and negatively related with ApoB,ApoE and ApoB/AI.Conclusion There was a close relationship of cardiovascular risk factors score with serum ApoB/AI,ApoB and ApoE.