ABSTRACT
Objective To study the effect of temporary in vitro preservation of parathyroid on the activity of cells in the process of parathyroid auto-transplantation and function of postoperative in order to improve the survival rate of transplantation.Methods (1)Experimental rabbits for the study were randomly divided into three groups:group A,group B,group C,with 8 rabbits in each group.Then we remove the bilateral inferior parathyroid,in group A,the parathyroid glands were immediately formaldehyde-fixed;in group B and group C,the parathyroid glands were placed in normal saline in 4 ℃ and in room temperature (22-24 ℃) for 30 minutes respectively and then fixed;HE staining was performed on the left parathyroid glands to observe the morphology of the cells;Electron microscopic examination of the right parathyroid glands were performed to observe the ultrastructural changes of the cells.(2)Experimental rabbits were randomly divided into three groups:group D group E and group F,with 8 rabbits in each group,after total thyroidectomy,the double inferior parathyroid glands were took out,in group D,the parathyroid glands were immediately transplanted in bilateral anterior cervical muscles;in group E and group F,the parathyroid glands were placed in normal saline 4 ℃ and in room temperature (22-24 ℃) for 30 minutes respectively and then transplanted.All animals were monitored of serum calcium and PTH on preoperative 1 d and postoperative 1 d,3 d,5 d,7 d;the parathyroid was took out for HE staining to observed survival of parathyroid tissue and pathology damage when 7d after operation.Results (1) The normal parathyroid gland is mainly dominated by the chief cells,the nucleus of the chief cell was round and centered under electron microscope;there were no significant change in the morphology of parathyroid cells,and the mitoehondria of the cells were slightly swollen under 4 ℃;but the parathyroid gland cells were slightly swollen and partially vacuole degeneration,the morphology of the nucleus was irregular,and the mitochondria were extremely swollen and deformed,and the ridge was broken under room temperature.(2)three groups of rabbits after transplantation of parathyroid,serum calcium and PTH decreased significantly,and increased gradually,there was significant difference on the 7th day after the operaion between the two groups (P＜0.05);(3)in group D,the normal parathyroid cells densely distributed in the anterior cervical muscle tissue;in group E,A large number of parathyroid cells survived in muscle tissue,with some vacuolated;in group F,only part of healthy parathyroid ceils scattered in the muscle.Conclusion Parathyroid should be preserved in 4 ℃ normal saline during the operation,and the transplant should be completed in 30 minutes as far as possible.
ABSTRACT
Neoglaziovia variegata (Arr. Cam.) Mez is a Bromeliaceae native to the Caatinga, used for fiber extraction in the Northeast Region of Brazil. The antropic activity has place this species among the threatened ones. The objective of the work was to establish an in vitro propagation and conservation of caroá. Seeds were cultivated in MS medium in the presence or absence of light. In vitro germinated seedlings were multiplied in MS medium supplemented with the combinations 0.05 and 0.50 µM NAA and 2.2 and 4.4 µM BAP and KIN. The best percentages of germination were obtained with the seeds incubated in the presence of light. The highest multiplication ratio was obtained for the NAA (0,5 µM) + BAP (4,4 µM) treatment and the number of roots, with NAA (0.5 µM) + KIN (2.2 µM). Plant acclimatization presented differentiated results regarding the substrates tested. The conservation was established.
Neoglaziovia variegata é uma Bromeliaceae nativa da Caatinga, usada para extração de fibras na Região Nordeste do Brasil. A atividade antrópica coloca esta espécie entre as ameaçadas. O objetivo deste trabalho foi estabelecer uma propagação e conservação in vitro de caroá. Foram cultivadas sementes em meio MS na presença ou ausência de luz. Plântulas germinadas in vitro foram multiplicadas em meio MS suplementado com as combinações de 0,05 e 0.50 µM de NAA e 2.2 e 4.4 µM de BAP e KIN. Foram obtidas as melhores porcentagens de germinação com sementes incubadas na presença de luz. A taxa de multiplicação mais alta foi obtida no tratamento NAA (0,5 µM) + BAP (4,4 µM) e, o número de raízes, com NAA (0.5 µM) + KIN (2.2 µM). Aclimatização das plantas apresentou resultados diferenciados em relação aos substratos testados. A conservação foi estabelecida.