Application of near infrared spectroscopy for quality control of in-process Wuji Baifeng Pill / 中成药

AIM: To study the application of near infrared spectroscopy for quality control of in-process Wuji Baifeng Pill(Gallus Domesticus,Colla Cornus cervi,Carapax Trionycis,Otheca Mantidis,Radix et Rhizoma Ginseng,etc.). METHODS: Qualitative and quantitative methods for analysis of in-process product was developed by diffuse reflectance near infrared spectroscopy combined with chemometrics.Spectra of 95 samples of Tongren Wuji Baifeng Pill in-process and 19 raw material negative in-process products were collected.Similarity match model was built on the basis of spectra of 75 batches of qualified in-process products.Using this model,the similarity match values of other samples were calculated.Multivariate calibration models for paeonin and water content in in-process product were developed with partial least square algorithm. RESULTS: The results showed that the proposed similarity match model could reflect the quality characteristic of Tongren Wuji Baifeng Pills in-process and reveal the absence and presence of raw materials.The correlation coefficients of the calibration model were within 0.965 7 and 0.992 1 while the values of root mean square error of prediction were 0.005 5% and 0.45%,respectively. CONCLUSION: The experiment shows that the established method is accurate,fast and non-destructive,and can be applied to on-line detection.