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Objective To evaluate the effect of minocycline hydrochloride (MH)-loaded in-situ hydrogels on periodontitis of rats. Methods Ligation, high sugar diet, local vaccination of Porphyromonas gingivalis and intramusclar injection of acetic predniso-lone were combined to establish the periodontitis of rats. Whether periodontitis was established successfully or not was determined according to clinical examination and pathology after 4 weeks. 28 periodontitis rats were divided into the model group, the positive controll (MH ointment), the blank in-situ hydrogels (ISG) and MH-loaded ISG groups. The detection index included gingival index, bleeding index, bleeding on probing, and probing depth before treatment and 4 weeks after treatment, respectively. Results The inflame-matory and destruction degrees of periodontal tissues in the positive and MH ISG groups were better than those of the model and the blank groups. Conclusion The curative effect of MH-loaded ISG on periodontitis is obvious. It is expected to become a novel formulation to treat periodontitis in future.
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Objective To investigate the therapeutic effect of curcumin in situ hydrogels for treatment of vaginal candidiasis. Methods In situ hydrogels were prepared using poloxamer 407 and 188 as the gel matrix.Gelation temperature,gelation time and rheological characteristics were determined.Mice models with vaginal candidiasis were established.The animals were divided into six groups:the healthy mice group,the model group,the positive control group(ketoconazole cream),curcumin-loaded in situ hydrogels of 0.025,0.10 and 0.20 g/ml.Before treatment and after the fourth and eighth treatments,the vaginal smears were collected and Gram-stained.The vagina washings fluid of mice were collected to determine DNA of Lactobacillus.On 8thday,the mice were sacrificed and the vaginal tissues were excised for pathological detection.Results The gelation temperature and time of in situ hydrogels were 29℃and 2′30″,respectively.Rheological profiles demonstrated that it showed good retention effect in vivo.Compared with the model group, curcumin hydrogels of 0.025,0.10 and 0.20 g/ml could inhibit the growth of Candida albicans,inhibit vaginal mucosal edema,inflam?matory hyperplasia,and decrease the expression of inflammatory factors including TNF-α(P<0.01)and IL-1β(P<0.01).Howev?er,curcumin hydrogels could not influence the growth of lactobacillus in the vagina.It indicated that curcumin hydrogels did not de?pend on lactobacillus to treat fungal vaginitis.Conclusion Curcumin in situ hydrogels could inhibit candidiasis growth,decrease ede?ma of vaginal mucosa and inflammatory hyperplasia,and reduce the expression of inflammatory factors.It provides a novel choice for the treatment of clinical vaginal candidiasis.
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Objective To prepare thermosensitive in sitt hydrogels (ISG) of gabexate mesilate and establish quality control method. Methods ISGs were prepared using PF127 and PF68 as matrix, evaluated by gelling temperature, gelling time, gelling capacity in vitro, and determinated by HPLC. Results The gelling time of ISG was (1. 8 ±0. 2) min, with gelling temperature at 317. When the gel temperature rose to 31 7, the viscosity of ISG increased dramatically. The linear range of HPLC determination curve was 10 -350 μg/ml, with an average recovery of 99. 63% (RSD =0. 88%). Conclusion Preparation of the gel with controllable quality is simple and easy. The determination method is reliable for gabexate mesilate thermosensitive m sitt hydrogels.
ABSTRACT
Objective To prepare thermosensitive in situ hydrogels (ISG) of gabexate mesilate and establish quality control method.Methods ISGs were prepared using PF127 and PF68 as matrix, evaluated by gelling temperature , gelling time, gelling ca-pacity in vitro, and determinated by HPLC.Results The gelling time of ISG was (1.8 ±0.2)min, with gelling temperature at 31℃. When the gel temperature rose to 31℃, the viscosity of ISG increased dramatically .The linear range of HPLC determination curve was 10-350 μg/ml, with an average recovery of 99.63%( RSD=0.88%) .Conclusion Preparation of the gel with controllable quality is simple and easy .The determination method is reliable for gabexate mesilate thermosensitive in situ hydrogels .