Callus induction, Indirect Organogenesis and Plantlet Regeneration from different explants of Vernonia anthelmintica (L.) Willd

Present study was conducted to standardize callus development and indirect organogenesis from differentexplants of Vernonia anthelmintica (L.) Willd. Among, the various hormonal combinations tested: IndoleAcetic Acid (IAA) and BA and IAA and Kinetin combinations were found to be optimum for inducing callusingwithin a time span of 10 days. Best callus response was observed in 1.5 mg l−1 IAA and 1.5 mg l−1 BA,which produced white friable callus. Best indirect shoot organogenesis was observed in 4 mg l−1 BA and6 mg l−1 kinetin. Elongated shoots when transferred on to half strength Murashige and Skoog (MS) Mediumsupplemented with Indole-3-butyric acid (IBA), rooting was observed. MS medium fortified with 2 mg l−1 IBAshowed better rooting than all other concentrations tested. This concentration produced maximum number ofroots and maximum percentage of rooting. Plantlets developed by indirect organogenesis of V. anthelminticawere successfully acclimatized to field conditions

REGENERACION Y SENSIBILIDAD A MANOSA EN ENTRENUDOS DE PAPA (Solanum tuberosum spp. andígena Var Diacol Capiro). / REGENERATION AND MANNOSE SENSIBILITY IN POTATO INTERNODAL EXPLANTS (Solanum tuberosum spp. andígena Var Diacol Capiro)

Se estableció un sistema de organogénesis indirecta para la obtención de brotes múltiples a partir de segmentos internodales de la variedad Diacol Capiro. La ubicación de explantes en medio Murashige y Skoog (MS) suplementado con 2 mg/l de zeatina ribosido (ZR), 0,02 mg/l de ácido naftalenácetico (ANA) y 0,02 mg/l de ácido giberélico (AG3), permite la obtención de plántulas entre la séptima y novena semana con una efectividad del 80-100%. Mediante ubicación de explantes previamente cocultivados con la cepa LBA4404 de Agrobacterium tumefaciens que contiene el plásmido recombinante pNOV022, se verificó la utilidad del medio para procesos de transformación, obteniéndose tasas hasta del 100% de regeneración. Finalmente, con el objetivo de determinar el uso potencial de la manosa como agente selectivo en procesos de transformación, se evaluó el efecto de diferentes concentraciones de manosa sobre la viabilidad y capacidad regenerativa de explantes.

A system of indirect organogenesis for the multiple buds production from internode stem sections in Diacol Capiro variety was established. Explants on Murashige & Skoog (MS) medium with zeatine riboside (ZR) 2 mg/l, naftalenacetic acid (NAA) 0.02 mg/l and giberelic acid (GA3) 0.02 mg/l, produced plants ranging between 7 to 9 weeks with 80-100% effectiveness. In the same medium, explants infected with Agrobacterium LBA4404 strain which carries recombinant plasmid pNOV022, produced regeneration rates reached 100%, thus, the medium utility for trnsformation processes was verified. Finally, to determine the potential use of the mannose as selective agent in transformation processes, the effect of different mannose concentrations on explant viability and regenerative capacity was evaluated.