Discussion on Treatment of Recurrent Genital Herpes with Zhulingtang： Based on Thought of "Lower Energizer Resembling Drainage" / 中国实验方剂学杂志

Recurrent genital herpes is a sexually transmitted disease induced by herpes simplex virus（HSV）， mainly manifested as erythema， papules， vesicles， erosion， and exudation on the skin and mucosal membranes of the genital and anal area， accompanied by burning pain， or tingling. Factors leading to its recurrence are overworking， fever， alcohol consumption， infection， sexual activity， etc. At least 1 in 10 of the 15-49-year-old population are infected with herpes simplex virus-2. There is currently no available method to completely remove the herpes simplex virus and prevent its recurrence， and recurrence seriously affects the quality of life and psychological health of patients. According to the theory of traditional Chinese medicine （TCM）， the recurrence of genital herpes is mostly related to wind， dampness， heat， yin deficiency of liver and kidney， internal injuries due to emotional disorder， and dietary preference. TCM has advantages in reducing its recurrence rate. The lower energizer is closely related to skin diseases， and "lower energizer resembles drainage". Thus， the lower energizer is closely related to water and damp metabolism. Recurrent genital herpes occurs in the lower energizer and belongs to lower energizer diseases， and the fluid metabolism and qi movement in lower energizer are closely related to the occurrence of the disease. Based on thought of "lower energizer resembling drainage" and the clinical characteristics of recurrent genital herpes， this paper considered malfunction of lower energizer as its pathogenesis. Thus， smoothing the lower energizer according to the prevailing circumstances is particularly important and the treatment principle should be dispersing the pathogen from lower energizer without damaging yin. Zhulingtang （Treatise on Cold Damage and Miscellaneous Diseases） cures the difficulty in urination and thirst with desire to drink by smoothing the lower energizer. Thus， Zhulingtang was selected to treat recurrent genital herpes. This study is expected to provide a new idea for its clinical diagnosis and treatment.

Incidence and determinant of eclampsia and its associated complication in tertiary care hospital of Gujarat, India

Background: Eclampsia is one of common cause of maternal mortality in developing country like India. It can be detected and prevented if early ANC care is properly done. Mainstay of management in case of eclampsia is early delivery to improve the prognosis in terms of reducing maternal and perinatal morbidity and mortality.Methods: This prospective study was carried out in the department of obstetrics and gynaecology, at tertiary care centre in the state of Gujarat, for a period of two years from July 2012 to June 2014. With purposive sampling method all patients admitted with complain of eclampsia is included in study. Details are taken in predesigned, pre-validated and prescribed proforma.Results: Hospital based incidence of eclampsia in our study is 1.11%. Majority of the patient were unbooked. Eclampsia is a disease of young primigravida, specifically teenage primigravida. Seventy-five percentage of eclampsia cases occurred in the antenatal period, 14% in intranatal period and 11% in the postnatal period.Conclusions: In developing countries like India still eclampsia is major problem. Good antenatal care with increased antenatal visits may help in reducing the incidence. Increased incidence among young primigravida and low socio-economic status group provides the target group for medical measures.

Volatiles induction in response to mechanical damage is reduced by domestication in murtilla / La inducción de volátiles en respuesta al daño mecánico es reducida por la domesticación en murtilla

Volatiles compounds are involved in defensive induction against insects, playing an important role in insect-plant interaction being induced by response to mechanical damage. However, they could decrease according to the domestication degree in cultivated plants. Currently, it has been established that secondary metabolites are reduced due to the domestication process in murtilla. Hence, the follow question emerges: Are volatile organic compounds induced by mechanical damage reduced in cultivated murtilla plants in relation to wild plants? Two cultivated ecotypes and their respective wild counterparts were sampled. Volatiles compounds were obtained using Porapak-Q columns and analyzed by gas chromatography. Results showed that compounds as 2-hexanone, α-pinene, 2-thujene, 3-thujene and 1,8- cineole were more abundant in wild plants exposed to a mechanical damage than cultivated plants. Hence, these compounds have been associated to induced defense, these results suggest that domestication reduced the induction of defensive volatiles in cultivated murtilla in response to mechanical damage.

Los compuestos volátiles están implicados en la defensa inducida contra insectos, desempeñando un papel importante en esta interacción. Sin embargo, estos compuestos podrían disminuir según el grado de domesticación. Actualmente, se ha reportado que algunos metabolitos secundarios son reducidos en plantas de murtilla domesticadas. Por lo tanto, surge la siguiente pregunta de investigación: ¿Los compuestos orgánicos volátiles inducidos por el daño mecánico son reducidos en plantas cultivadas de murtilla en comparación con plantas silvestres? Para dos ecotipos cultivados y sus respectivas contrapartes silvestres, los compuestos volátiles fueron capturados usando columnas de Porapak-Q y las muestras analizadas por cromatografía gaseosa. Los resultados mostraron que compuestos tales como 2- hexanona, α-pineno, 2-tujeno, 3-tujeno y 1,8-cineol fueron más abundantes en plantas silvestres expuestas a daño mecánico que en cultivadas. Debido a que estos compuestos se han asociado a defensa inducida, estos resultados sugieren que la domesticación reduce la inducción de volátiles en plantas cultivadas sometidas a daño mecánico.

Effect of GR24 on accumulation of diterpenoids in Tripterygium wilfordii suspension cells / 中国中药杂志

Terpenoids are main bioactive components in Tripterygium wilfordii,but the contents of some terpenoids are relatively low. In order to provide scientific evidence for the regulation of terpenoids in T. wilfordii,this research explored the effect of GR24 on accumulations of four diterpenoids( triptolide,tripterifordin,triptophenolide,and triptinin B) in T. wilfordii suspension cells by biological technology and UPLC-QQQ-MS/MS. The results indicated that 100 μmol·L-1 GR24 inhibited the accumulations of triptolide,tripterifordin,triptophenolide,and triptinin B to different degrees. Compared with the control group,the contents of 4 diterpenoids( in the induced group) were down to 96.59%,63.80%,61.02% and 33.59% in 240 h,respectively. Among them,the accumulation of triptinin B iswas significantly inhibited. In addition,the key time point of inhibitory effect was 120 h after induction with GR24 in some diterpenoids. This is the first systematic study focusing on the effect of GR24 on the accumulations of diterpenoids in T. wilfordii suspension cells. The dynamic accumulation ruleregularity of four diterpenoids after induced by GR24 was summarized,which laid a foundation for further study on the chemical response mechanism of terpenoids to GR24.

Relationship between radiation-induced xerostomia and the volume of parotid glands exposed in radiotherapy for nasopharyngeal carcinoma / 中华放射肿瘤学杂志

Objective To evaluate the relationship between the volume of parotid glands and radiation-induced xerostomia in patients with nasopharyngeal carcinoma(NPC). Methods A total of 199 patients with NPC who were admitted to our hospital from 2015 to 2016 were enrolled as subjects. According to the initial volume of parotid glands, all patients were divided into large parotid group and small parotid group. The incidence of xerostomia after radiotherapy and dosimetric parameters were compared between the two groups. Comparison of categorical data was made by x2 test. Comparison of continuous data was made by t-test or nonparametric test. Comparison of dose-volume histogram index was made by nonparametric test. Results There was no difference in the severity of xerostomia between the two groups at 3 and 6 months after radiotherapy. At one year after radiotherapy,the large parotid group had significantly milder xerostomia than the small parotid group(P=0.035).The small parotid group had a higher dose delivered to both parotid glands than the large parotid group. There was no difference in the mean dose to the submandibular gland between the two groups. Conclusions The initial volume of parotid glands is one of the influencing factors for the grade of xerostomia after radiotherapy. Patients with large parotid glands have better recovery from xerostomia after radiotherapy than those with small parotid glands. For patients with small parotid glands, more attention should be paid to reducing the dose to protect parotid glands.

Induction of Defense-Related Physiological and Antioxidant Enzyme Response against Powdery Mildew Disease in Okra (Abelmoschus esculentus L.) Plant by Using Chitosan and Potassium Salts

Foliar sprays of three plant resistance inducers, including chitosan (CH), potassium sorbate (PS) (C₆H₇kO₂), and potassium bicarbonates (PB) (KHCO₃), were used for resistance inducing against Erysiphe cichoracearum DC (powdery mildew) infecting okra plants. Experiments under green house and field conditions showed that, the powdery mildew disease severity was significantly reduced with all tested treatments of CH, PS, and PB in comparison with untreated control. CH at 0.5% and 0.75% (w/v) plus PS at 1.0% and 2.0% and/or PB at 2.0% or 3.0% recorded as the most effective treatments. Moreover, the highest values of vegetative studies and yield were observed with such treatments. CH and potassium salts treatments reflected many compounds of defense singles which leading to the activation power defense system in okra plant. The highest records of reduction in powdery mildew were accompanied with increasing in total phenolic, protein content and increased the activity of polyphenol oxidase, peroxidase, chitinase, and β-1,3-glucanase in okra plants. Meanwhile, single treatments of CH, PS, and PB at high concentration (0.75%, 2.0%, and/or 3.0%) caused considerable effects. Therefore, application of CH and potassium salts as natural and chemical inducers by foliar methods can be used to control of powdery mildew disease at early stages of growth and led to a maximum fruit yield in okra plants.

The inhibitory effect of isoquercitrin onRaf/MEK/ERK signaling pathway in HepG2 cells / 中国药理学通报

Aim To study the inhibitory effect of isoquercitrin on Raf/MEK/ERK signaling pathway in HepG2 cells.Methods MTT was used to detect the proliferation of human liver cancer HepG2 cells after the treatment of isoquercitrin.The morphology and growth of cells were observed under inverted microscope after the different concentrations of isoquercitrin(0, 40, 80, 160, 320 μmol·L-1) to treat HepG2 cells for 24 and 48 h.Cell cycle was assessed by flow cytometry.Ras, Raf, MEK, ERK expression was assayed by Western blot, and mRNA expression was detected by quantitative fluorescence PCR.Results Isoquercitrin could inhibit the growth of HepG2 cells in a concentration-and time-dependent manner.Typical morphological changes of apoptosis were observed by inverted microscopy after HepG2 cells were treated with different concentrations of of isoquercitrin for 24 h or 48 h.The cell cycle assay showed that with the increasing concentration of isoquerditrin, the number of cells that was arrested in G1 phase gradually increased.Compared with the blank group, the expressions of Ras, Raf, MEK, ERK mRNA were down-regulated, and related proteins expression were also down-regulated(P<0.05), and these results had statistical significance.Conclusion Isoquercitrin can induce the apoptosis of HepG2 cells, which may be related to the Raf/MEK/ERK signaling pathway.

Analysis of anorectal function in cervical cancer patients with chronic pelvic radiation disease / 医学研究生学报

[Abstract ] Objective Pelvic radiation disease(PRD) is multiple injuries in more than one organ resulted by pelvic radio-therapy.Patients have the symptoms of frequent feces and fecal incontinence after the resection of small bowel lesion .Sugeries on PRD patients were mainly distal ileum and ilieocecal valve resections , while the most susceptible part of pelvic radiation injury is rectum . However , little research has been done concerning PRD patients′anorectal functions .This study was mainly to evaluate the anorectal function of cervical cancer patients with PRD in order to provide evidence for the therapy and prognosis of PRD . Methods Cervical cancer patients with PRD in need of small bowel resection who hospitalized in our department from January 2014 to January 2015 were collected as patient group , while people from outpatient physical exam group were selected as control group according to the exclusion criteria of hypertension , diabetes, constipation and unrelaxed pelvic floor syndrome .PDR group and control group were matched according to age.All subjects underwent colonoscopy and anorectal manometry .Rectal radiation injury was estimated on the basis of colonoscopy results.Anorectal manometry results of PRD group and control group were analysised statistically . Results PRD group and control group both included 20 women without stenosis or obstruction in rectum .Significant difference was found between PRD group and con-trol group in anal resting pressure (47.23 ±9.08 mmHg vs 58.25 ±9.24 mmHg, P<0.05), anal maximum squeezing pressure (47.23 ±9.08 mmHg vs 58.25 ±9.24 mmHg, P<0.01), anal distension pressure (23.30 ±12.49 mmHg vs 39.10 ±9.99 mmHg, P<0.01), rectal defecation pressure(22.85 ±16,69 mmHg vs 50.90 ±9.14 mmHg, P<0.01) and maximum tolerated rectal volume (112.85 ±51.34 mL vs 173.50 ±48.15 mL, P<0.01).There was no significant difference between the two groups as to the lenghth of functional sphincters(P=0.313),rectum initial threshold(P=0.416) and rectal defecation threshold(P=0.161). Conclusion Ionization radiation that injures PRD patients′internal anal sphincters and external anal sphincters also reduces maximum tolerated rec -tal volumes preoperatively .It′s necessary to assess the muscles and nerve functions of anorectum before intestinal surgery in order to make a proper operation plan which will improve PRD patients′life quality.

Advances of TGF-β-induced long non-coding RNAs in tumors / 中国肿瘤临床

The TGF-βsignaling pathway plays a crucial role in regulating cell proliferation, differentiation, and apoptosis. This pathway exerts either tumor-suppressing or tumor-promoting effects, which are cell-or context-dependent, making it simultaneously advanta-geous and disadvantageous in the process of carcinogenesis and tumor progression. Long non-coding RNAs (lncRNAs) do not encode proteins, but they are involved in the regulation of various signaling pathways and biological functions. Moreover, lncRNAs can induce tumor angiogenesis, as well as affect tumor proliferation, invasion, and metastasis by acting as oncogenes or tumor-suppressor genes. Recent studies revealed that some lncRNAs may be induced and regulated by TGF-βto form a complicated crosslinked regulatory net-work. This review focuses on the crosstalk between the TGF-βsignaling pathway and TGF-β-induced lncRNAs.

Taste from the Matching of Qingyingtang Prescription Features / 浙江中医药大学学报

Objective]To investigate the characteristics of Qingyingtang group side, learning worthy dialectical medication characteristics, to guide clinical practice. [Methods] Retrieve ancient and modern literature, combined with a comprehensive analysis of drug-induced flavor theory;drug potency and taste of Qingyingtang were demolished square analysis, summary described the taste Qingyingtang prescription characteristics. [Results] Analyse Qingyingtang prescriptions from the taste characteristics of compatibility aspects, it can be summarized as follows:salty Hanku vent, Qingreliangxue, Gan Han Zi camp, nourish lung and stomach, bitter cold stasis, blood stasis and new students, Xin Liang Qing Xuan, through heat transfer gas four aspects. Wu Jutong heat into the blood according to the clinical syndrome features, combined with the taste of the above diagnosis and treatment using the compatibility Herbal Prescription for TCM Syndrome methods election due to drug model, called the lead from future generations. [Conclusion] The taste of the theory of the clinical treatment combination prescription medication an important basis for Qingyingtang nature flavor compatibility demolition fomula research can enhance the the world of knowledge and understanding of compatibility, thereby improving clinical scholar ability prescription medication.

Study on osteogenic/chondrogenic differentiation potential of cartilage endplate derived stem cells in vivo / 局解手术学杂志

Objective To observe the osteogenic and/or chondrogenic differentiation potential of cartilage endplate derived stem cells ( CESCs) .Methods The cartilage endplate ( CEP) was obtained from 5 patients who underwent posterior discectomy procedure for the lumber degenerative disease.The agarose culture was used to select CESCs.The expanded CESCs were injected into the hydroxyapatite ceram-ic(HA) and incubated for 7 days.After that,the HA contained CESCs were implanted into the subcutaneous of nude mouse for 7 weeks.Then the HA was obtained and sliced for the specific stain and immunohistochemisty.The expanded CESCs were injected into the degenerated in-tervertebral disk of the rabbit for 16 weeks.Then MRI was used to detect the repair of the degenerated disc.Results The metachromasia of toluidin blue indicated that cartilage matrix was formed in the vacant space of HA.Mallory stain indicated that there was bone matrix was formed.The results of immunohistochemistry indicated that there was more collageⅠand collageⅩ,and a small quantity of collage Ⅱ.MRI results indicated that the CESCs could be induced into the chondrocyte-like cells in the rabbit intervertebral disk.Conclusion The CESCs could be induced into the bone/cartilage in vivo.

Progress of stress-induced flowering in plants / 生物工程学报

Plants tend to flower earlier if placed under stress conditions. Those stress factors include drought, high salinity, low temperature, high- or low-intensity light, and ultraviolet light. This phenomenon has been called stress-induced flowering. Stress-induced plant flowering might be helpful for species preservation. Thus, stress-induced flowering might have biological significance and should be considered as important as other plant flowering control strategy. Here, history of stress-induced flowering, metabolic regulation and molecular regulation mechanisms in plants were reviewed. Potential perspective was discussed.

A pilot study on differentiation of human amnion mesenchymal stem cells into ocular surface epithelial cells by co-culture in vitro / 中华实验眼科杂志

Background Recent studies indicated that human amnion mesenchymal stem cells (hAMSCs) can be induced to differentiate into multiple types of cells in vitro,but whether the hAMSCs can differentiate into ocular surface cells has not been reported yet.Objective This study was to investigate the feasibility of inducing differentiation of hAMSCs into ocular surface cells by co-culturing with human bulbar conjunctiva fibroblasts (hBCFs).Methods This study was approved by Ethic Committee of Affiliated Second Hospital of Guangzhou Medical University.HAMSCs were isolated from placenta under the informed consent of healthy delivery women.hAMSCs were cultured,passaged and identified by detecting the expressions of CD44,CD45,CD73,CD90 in the cells with flow cytometer,osteogenesis and adipogenic differentiation experiments.Human conjunctival tissue was obtained during the eye operation under the informed consent of patients and hBCFs were isolated and cultured with explant culture.The cells were divided into the hAMSCs culture group and the hAMSCs and hBCFs co-culture group and cultivated in Transwell chambers for 7 days.The expressions of cytokeratin 19 (CK19) and α-smooth muscle actin (α-SMA) in the cells were assayed by immnofluorescence technique.Results Cultured hAMSCs showed the slender shape and cell body enlarged with passage.CD44,CD73 and CD90 were expressed in the cells,and the expression of CD45 was absent.After 3-4 weeks of osteogenesis and adipogenic induce,the cells showed red staining for alizarin and oil red O.In the co-culture group of hAMSCs and hBCFs,hAMSCs presented the epithelioid cell-like in shape and showed the positive response for CK19 and weaker response for α-SMA.However,in the hAMSCs culture group,the cells showed the positive response for α-SMA and absent response for CK19.Conclusions The hAMSCs can differentiate into ocular surface cells after being induced by hBCFs.And the differentiation mechanism is possibly relevant to mesenchymal cells epithelium.

Impact study of curcumin on ASO and IL-8 level changes in serum of psoriatic mice model induced by TPA / 中国生化药物杂志

Objective To explore the impact of curcumin on antistreptolysin“O”(ASO)and interleukin 8 (IL-8)level changes in serum of psoriatic mice model induced by phorbol ester(TPA).Methods 132 SCID mice were selected and divided into three groups.43 mice in normal group, 46 mice in model group,and 43 mice in intervention group.The mice of model group and intervention group were set up psoriasis model induced by TPA. Mice of the three groups were bred in the same environment,curcumin was injected in the intervention group mice,0.02 g/100 g,once a day,two weeks for a period of treatment.ASO and IL-8 level were compared amorg the three groups of mice after treatment.Results Before the treatment,compared with the normal group,positive rate of ASO in model group and intervention group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,the level of intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05 );before the treatment,compared with the normal group,IL-8 level in intervention group and model group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,level of the intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05).Conclusion Curcumin could effectively reduce ASO and IL-8 level in serum of psoriatic mice model, improve the clinical symptoms of psoriasis mice,it is an effective drug for treatment of psoriasis.

Comparative proteomic analyses on the differentiation of dimethyl sulfoxide induced murine erythroleukemia cells / 解剖学报

Objective To explore the differentially expressed proteins during erythroid differentiation .Methods The murine erythroleukemia ( MEL) cell were treated by DMSO , and the comparative proteomic was systematically analyzed and identified on different differentiating time points .ratio of cell differentiation and viability were detected by benzidine staining, MTT assay and Ter119 immunofluorescence.Using two-dimensional gel electrophoresis combined with mass spectrometry technology and bioinformatics analysis , we conducted a comparative proteomic analysis on MEL cells during the process of induced differentiation to screen and identify differential proteins .Results The MEL cells induced by 1.2%DMSO for 0 hour, 6hours, 12hours, 24hours, 36hours, 48hours, 72 hours, 96 hours, 120 hours were collected for proteomic analysis, by two-dimensional gel electrophoresis combined with mass spectrometry .A total of 87 kinds of proteins were successfully identified .MEL cells exposed to DMSO at a final concentration of 1.2% for 120 hours reached the highest differentiation rate of 67%.MTT assay showed that 1.0%, 1.2%, 1.4% DMSO had no inhibiting effect on cell vitality.Conclusion DMSO may induce MEL cells to differentiate and have no inhibiting effect on cell vitality .The 87 kinds of differentially expressed proteins from two-dimentional gel electrophoresis may be divided into twelve categories ;the most three parts are 41%enzyme protein, 15%structural protein and 13%regulatory protein.

Lesões parasitárias encontradas em bovinos abatidos para consumo humano / Parasitic lesions observed in cattle slaughtered for human consumption

During a period of 28 months (January 2011-April 2013) periodical visits were made to four cattle slaughter houses to collect samples of lesions found in cattle slaughtered for human consumption. Three hundred and three lesions were collected, 25% of which were caused by six different parasites: The metacestodes of Echinocccus granulosus (hydatid cysts), Fasciola hepatica, the metacestodes of Taenia saginata (Cysticercus bovis), Oesophagostomum radiatum, Eurythrema coelomaticum, and Paramphistomum cervi. Gross and microscopic aspects of the lesions caused by these worms are profusely illustrated and a detailed description of both pathological aspects, gross and microscopic, are provide in the hopes that the neophyte veterinary meat inspector can benefit from them in the task of recognition and interpretation of the significance of these lesion both to human and animal health. In regard to the two latter objectives, the life cycle, and the pathogenicity to the animal and human hosts, and the destination of the affected carcass or organ in the line of inspection are discussed for each parasite.

Durante um período de 28 meses (janeiro 2011-abril 2013) foram feitas visitas periódicas a quarto abatedouros frigoríficos de bovinos para colher lesões regularmente encontradas em bovinos abatidos para consumo humano. Trezentas e três lesões foram colhidas, 25% das quais eram causadas por seis parasitas diferentes: o metacestoide de Echinocccus granulosus (cisto hidático), Fasciola hepatica, o metacestoide de Taenia saginata (Cysticercus bovis), Oesophagostomum radiatum, Eurythrema coelomaticum e Paramphistomum cervi. Os aspectos macro e microscópicos causados por esses vermes são profusamente ilustrados e uma descrição macro e microscópicadas lesões é fornecida na esperança de auxiliar o inspetor de carnes iniciante na tarefa de reconhecer e interpretar o significado dessas lesões tanto para a saúde animal quanto para a saúde humana. Com respeito a esses últimos objetivos, o ciclo evolutivo, e os efeitos nos hospedeiros animal e humano, e ainda a destinação da carcaça ou órgão afetado na linha de inspeção, são discutidos para cada parasita.

Discussion on acupuncture promoting child delivery theory / 国际中医中药杂志

From ancient times to the present,acupuncture has made tremendous contributions to women' s reproductive health.Based on traditional Chinese medicine of kidney-menstruation-Chong and Ren-uterus axis and Western medicine of hypothalamus-pituitary-ovarian axis comparative analysis study,try to explore possible ways from the acupuncture point to molecular changes in the process of acupuncture and moxibustion treatment.

Observation on the effect of modified long-term low concentration caudal blocking in medium-term drug induced odinopoeia / 中国基层医药

Objective To explore the effects of modified long-term low concentration caudal blocking in medium-term drug induced odinopoeia.Methods According to the digital table,63 patients received drug induced odinopoeia were divided randomly into the control group(without analgesis)and treatment group(analgesis).The control group were treated with caudal blocking with mifepristone,premelle and ethacridine lactate.The treatment group received additional treatments of 0.75％ bupivacaine hydrochloride + 2％ lidocaine hydrochloride + morphine sulfate injection based on treatment of the control group.The duration of labor,bleeding volume and severity of pain were recorded and compared between the two groups.Results The duration of labor of the treatment group was (5.33 ±3.77) h,which was significantly shorter than that of the control group [(7.88 ± 2.62) h] (t =8.01,P ＜ 0.05).The severity of pain of the treatment group were all better than those of the control group(all P ＜0.05).There was no significantly statistificant difference in bleeding volume between the two groups (t =4.63,P ＞ 0.05).Conclusion Application of modified long-term low concentration caudal blocking in medium-term drug induced odinopoeia has confirmed analgesis effects,and can shorten duration of labor without increasing bleeding volume.

Research progress of retinoic acid in treatment of differentiated thyroid carcinoma / 肿瘤研究与临床

Differentiated thyroid carcinoma (DTC) cells can produce loss of differentiation in the progress of cancer development,especial of metastasis.The morphology and function of loss of differentiation DTC cell both generate degenerative changes,resulting in the reduction or loss of iodine uptake of the radioactive,which make radioactive iodine(131Ⅰ) treatment and thyroid hormone suppression therapy inefficient on treating DTC.Retinoic acid(RA) is a biologically active metabolite of vitamin A,which plays an important role in many cell morphology,degree of differentiation and proliferation process.Studies have shown that the application of RA can induce the loss differentiation of DTC redifferentiation,make its to reverse for functional thyroid organization.It’s important to produce the clinical significance,which can improve the cure rate and survival rate of the DTC patients.

Delphinidin inhibits cell proliferation and induces apoptosis in MDA-MB-231 human breast cancer cell lines / 한국영양학회지

Breast cancer is the most common malignancy in women, both in the developed and developing countries. Anthocyanins are natural coloring of a multitude of foods, such as berries, grapes or cherries. Glycosides of the aglycons delphinidin represent the most abundant anthocyanins in fruits. Delphinidin has recently been reported to inhibit the growth of human tumor cell line. Also, delphinidin is a powerful antioxidant that reportedly exerts beneficial effects in patients with advanced cancer by reducing the level of reactive oxygen species and increasing glutathion peroxidase activity. This study investigates the effects of delphinidin on protein ErbB2, ErbB3 and Akt expressions associated with cell proliferation and Bcl-2, Bax protein associated with cell apoptosis in MDA-MB-231 human breast cancer cell line. MDA-MB-231 cells were cultured with various concentrations (0, 5, 10, and 20 micromol/L) of delphinidin. Delphinidin inhibited breast cancer cell growth in a dose dependent manner (p < 0.05). ErbB2 and ErbB3 expressions were markdly lower 5 micromol/L delphinidin (p < 0.05). In addition, total Akt and phosphorylated Akt levels were decreased dose-dependently in cells treated with delphinidin (p < 0.05). Futher, Bcl-2 levels were dose-dependently decreased and Bax expression was significantly increased in cells treated with delphinidin (p < 0.05). In conclusion, I have shown that delphinidin inhibits cell growth, proliferation and induces apoptosis in MDA-MB-231 human breast cancer cell lines.