ABSTRACT
[Objective]To investigate the effect of injectable xuebijing on macrophage ofimervertebral disc hernial tissue.[Method]The pigs were used as animal models after surgery in the intervertebral disc.Macrophage in herniated disc tissue were observed with the immunohistochemical method after it was treated by injectable xuebijing.[Result]Macrophage were found in 11 of 16 herniated disc tissue in the model group,were found in 7 of 16 herniated disc tissue in the low dose xuebijing group,and were found in 6 of 16 herniated disc tissue in the high dose xuebijing group,compared to the model group,all P
ABSTRACT
Objective To investigate the effect of injectable Xuebijing on the proliferation of mouse brain microvascular endothelial cell in vitro.Methods Cultured mouse brain microvascular endothelM cell line bEnd. 3 was treated by injectable Xuebijing of different concentrations,0 (control),5,25 and 50 mg/ml.The regulatory. effect of Xuebijing on the proliferation of cell line bEnd.3 was observed and studied by means of MTT method and cell cycle analyzed with flow cytometry.Results Compared to the control group,MTT and proliferation index (PI) of 5 and 25 mg/ml groups were significantly increased at 12 and 24 h,and PI,but not MTT,of these 2 groups was decreased remarkably at 48 h.Meanwhile,50 mg/ml group showed significantly decreased MTT at 24 and 48 h,and PI of this group was increased obviously at 12 and 24 h,but decreased significantly at 48 h. Conclusions Injectable Xuebijing at certain concentrations might promote the proliferation of cultured mouse brain microvascular endothelial,cells within specific time frame.