ABSTRACT
OBJECTIVE@#To observe the effect of electroacupuncture (EA) on the expression of insulin phosphatidylinositol-3 kinase/glycogen synthetase kinase-3α (PI3K/GSK3α) signal pathway related proteins in the hippocampus in mice with Alzheimer's disease (AD), and to explore the regulatory mechanism of EA on improving the pathological characteristics of AD.@*METHODS@#Twelve male APP/PS1 double transgenic mice were randomly divided a model group and a treatment group, 6 mice in each group; another 6 wild-type male mice were taken as the control group. The mice in the treatment group were treated with EA (continuous wave, 2 Hz of frequency) at "Baihui" (GV 20) and bilateral "Shenshu" (BL 23), once a day; 7-day treatment was taken as a course of treatment, and 2 courses of treatment were given. The immunohistochemistry method and Western blot method were used to detect the distribution and expression level of hippocampal PI3K/GSK3α signal pathway related proteins P85α, P110α, GSK3α and pSGSK3α, and the number of hippocampal senile plaques (SP) was observed.@*RESULTS@#The proteins of P85α, P110α, GSK3α and pSGSK3α were mainly distributed in the cytoplasm of hippocampal neurons, and the GSK3α was also distributed in the axons of neurons in the model group and the treatment group. The immunohistochemistry results showed that the distribution level of GSK3α in the hippocampus in the model group was significantly higher than that in the control group (<0.001), and the distribution level of pSGSK3α, P85α and P110α was significantly decreased (<0.01, <0.001); compared with the model group, the distribution level of GSK3α in the hippocampus in the treatment group was significantly decreased (<0.001), and the distribution level of pSGSK3α, P85α and P110α in hippocampus was significantly increased (<0.05, <0.001). The Western blot results showed compared with the control group, the expression of pSGSK3α, P85α and P110α as well as the ratio of pSGSK3α/GSK3α in the hippocampus in the model group were significantly decreased (<0.001), and the expression of GSK3α was increased (<0.05); compared with the model group, the expression of pSGSK3α, P85α, P110α and the ratio of pSGSK3α/GSK3α in the hippocampus in the treatment group were significantly increased (<0.01, <0.001), and the expression of GSK3α was decreased (<0.05). Compared with the control group, the number of hippocampal SP in the model group was significantly increased (<0.001); compared with the model group, the number of hippocampal SP in the treatment group was significantly decreased (<0.01).@*CONCLUSION@#EA could effectively regulate the expression of PI3K/GSK3α signal pathway related proteins in the hippocampus in mice with AD, so as to reduce the formation and deposition of SP.
Subject(s)
Animals , Male , Mice , Alzheimer Disease , Therapeutics , Electroacupuncture , Hippocampus , Physiology , Insulin , Physiology , Mice, Transgenic , Random Allocation , Signal TransductionABSTRACT
AIM To explore the therapeutic effects of polysaccharide from Anemarrhena asphodeloides Bunge (PAA) on diabetic rats and potential mechanism of action.METHODS The rat model for diabetes was established by intraperitoneal injection of STZ (60 mg/kg),and sixty rats were assigned to control-,model-,glibenclamide-(25 mg/kg) groups and three groups of PAA-(50,100,200 mg/kg).Drugs were intragastrically administrated to rats once a day for 28 days.The rat body weight,glucose tolerance,fasting blood glucose (FBG),fasting insulin (FINS),IL-6,TNF-α,CAT,SOD,MDA,insulin receptor substrate (IRS1),Phospho-IRS1 and glucose transporter protein 4 (Glut4) were tested.RESULTS PAA could increase rat body weight and FINS level,reduce FBG level,and improve the glucose tolerance.The levels of IL-6 and TNF-α in serum,and MDA content in hepatic tissue were decreased,and the activities of CAT and SOD in hepatic tissue were increased.Meanwhile,PAA could also reduce the Phospho-IRS1 expression,and increase the Glut4 expression in hepatic tissue.CONCLUSION PAA has an anti-diabetic effect,whose mechanism is involved in anti-inflammatory,antioxidation,decreasing Phospho-IRS1 expression,and increasing Glut4 expression.
ABSTRACT
AIM To explore the therapeutic effects of polysaccharide from Anemarrhena asphodeloides Bunge (PAA) on diabetic rats and potential mechanism of action.METHODS The rat model for diabetes was established by intraperitoneal injection of STZ (60 mg/kg),and sixty rats were assigned to control-,model-,glibenclamide-(25 mg/kg) groups and three groups of PAA-(50,100,200 mg/kg).Drugs were intragastrically administrated to rats once a day for 28 days.The rat body weight,glucose tolerance,fasting blood glucose (FBG),fasting insulin (FINS),IL-6,TNF-α,CAT,SOD,MDA,insulin receptor substrate (IRS1),Phospho-IRS1 and glucose transporter protein 4 (Glut4) were tested.RESULTS PAA could increase rat body weight and FINS level,reduce FBG level,and improve the glucose tolerance.The levels of IL-6 and TNF-α in serum,and MDA content in hepatic tissue were decreased,and the activities of CAT and SOD in hepatic tissue were increased.Meanwhile,PAA could also reduce the Phospho-IRS1 expression,and increase the Glut4 expression in hepatic tissue.CONCLUSION PAA has an anti-diabetic effect,whose mechanism is involved in anti-inflammatory,antioxidation,decreasing Phospho-IRS1 expression,and increasing Glut4 expression.