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ObjectiveTo explore the mechanisms of internal treatment （Renshen Baidusan）， external treatment （Yurui Enema）， and combination of the two methods in treating intestinal mucosal injury in the rat model of ulcerative colitis （UC） from the changes of phosphatidylinositol-3 kinase （PI3K）/protein kinase B （Akt）/nuclear factor-κB （NF-κB） pathway. MethodFifty SPF-grade SD rats were randomized into blank， model， Renshen Baidusan （15.6 g·kg-1）， Yurui Enema （25 g·kg-1）， and combined treatment （15.6 g·kg-1 Renshen Baidusan + 25 g·kg-1 Yurui Enema） groups （n=10）. The rat model of UC was established in other groups except the blank group by 2，4， 6-trinitrosulfonic acid （TNBS）/ethanol. The rats were administered with corresponding drugs once a day for 14 consecutive days since the 8th day after modeling. The histopathological changes of colon were observed by hematoxylin-eosin （HE） staining. Enzyme-linked immunosorbent assay （ELISA） was employed to measure the levels of tumor necrosis factor （TNF）-α， interferon （IFN）-γ， interleukin （IL）-4， and IL-10 in the colon tissue. The apoptosis of colon epithelial cells was detected by terminal deoxynucleotidyl transferase-mediated nick end labeling （TUNEL）. The location and expression of B-cell lymphoma-2 （Bcl-2）， Bcl-2-associated X protein （Bax）， TNF-α， and IL-6 in the colon tissue were examined by immunohistochemistry. Real-time quantitative fluorescence polymerase chain reaction （Real-time PCR） and Western blot were employed to determine the mRNA and protein levels， respectively， of the proteins in the PI3K/Akt/NF-κB pathway in the colon tissue. ResultIn the model group， HE staining showed a large number of inflammatory cell infiltration in the mucosa and submucosa. Compared with the blank group， the model group showed elevated levels of TNF-α and IFN-γ and lowered levels of IL-4 and IL-10 in the colon tissue， increased apoptosis rate of colon epithelial cells， increased positive expression of Bax， TNF-α， and IL-6， and decreased positive expression of Bcl-2 （P<0.05）. Moreover， the model group showed up-regulated mRNA levels of PI3K， Akt， and NF-κB and protein levels of PI3K， p-PI3K， Akt， p-Akt， p65， p-p65， Bax， and cleaved Caspase-3， increased Bax/Bcl-2 and cleaved Caspase-3/Caspase-3 ratios， and down-regulated protein levels of NF-κB suppressor protein α（IκBα）， Bcl-2， and Caspase-3 in the colon tissue （P<0.05）. Compared with the model group， the internal treatment， the external treatment， and the combination （referred to as the three groups） alleviated the colonic mucosal injury， lowered the levels of TNF-α and IFN-γ and elevated the levels of IL-4 and IL-10 in the colon tissue， decreased the apoptosis rate of colon cells， inhibited the positive expression of Bax， TNF-α， and IL-6， and promoted the positive expression of Bcl-2 （P<0.05）. Furthermore， the combination group down-regulated the mRNA level of PI3K （P<0.05）. The three groups down-regulated the mRNA levels of Akt and NF-κB and the protein levels of p-PI3K， Akt， p-Akt， p65， p-p65， Bax， and cleaved Caspase-3 in the colon tissue， decreased the Bax/Bcl-2 and cleaved Caspase-3/Caspase-3 ratios， and up-regulated the protein levels of IκBα， Bcl-2， and Caspase-3 （P<0.05）. ConclusionRenshen Baidusan， Yurui Enema， and their combination may inhibit the activation of PI3K/Akt/NF-κB signaling pathway and regulate the expression of genes and proteins related to this pathway to achieve anti-inflammatory and anti-apoptotic effects， thus restoring the intestinal mucosal barrier function of UC rats.
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Objective:To evaluate the effect of Hibiscus paste external application combined with traditional Chinese medicine on the inflammatory markers in patients with active rheumatoid arthritis (RA). Methods: The patient information was collected by the data processing system of hospitalized medical records in the First Affiliated Hospital of Anhui University of traditional Chinese medicine. The medical records of patients with rheumatoid arthritis in the Department of Rheumatology from May 2012 to December 2016 were collected. Patients were divided into experimental group and control group according to whether Furong ointment was applied. The random walk model was used to evaluate the influence of Furong ointment combined with traditional Chinese medicine on inflammatory index,erythrocyte sedimentation rate ( ESR) and high sensitivity C reactive protein ( Hs-CRP) in patients with active rheumatoid arthritis. Results: A total of 4 832 patients with rheumatoid arthritis were in accordance with the requirements of the study, including 2 579 in the experimental group and 2 253 in the control group. Baseline data analysis showed that there was no significant difference in general condition, frequency of oral Chinese medicine and core prescriptions between two groups ( P＞0. 05 ) . In the experimental group,the maximum ESR of inflammation index was 968,the walking rate was 3 756,the positive growth rate of walking was 0. 257 7,the ratio was 3. 88,the random fluctuation power law value was 0. 364 2±0. 124 6,the comprehensive evaluation index in-creased by 0. 479 9,and the comprehensive evaluation index was 2 017 times. In the control group,the maximum ESR of inflammation index was 398,the number of walking steps was 2 251,the positive growth rate of walking was 0. 176 8,the ratio was 5. 66,the random fluctuation power law value was 0. 173 5±0. 128 8,the comprehensive evaluation index increased by 0. 357,and the comprehensive evaluation index was 1 115 times. In the experimental group,the maximum Hs-CRP of inflammation index was 1 523,the walking rate was 5 149,the positive growth rate of walking was 0. 295 8,the ratio was 3. 38,the random fluctuation power law value was 0. 389 5± 0. 108 1,the comprehensive evaluation index increased by 0. 537 4,and the comprehensive evaluation index was 2 834 times. In the control group,the maximum Hs-CRP of inflammation index was 809,the number of walking steps was 3 463,the positive growth rate of walking was 0. 233 6,the ratio was 4. 28,the random fluctuation power law value was 0. 362 9±0. 073 8,the comprehensive evaluation index increased by 0. 448 2,and the comprehensive evaluation index was 1 805 times. Conclusion: Our hospital rheumatology on the treatment of rheumatoid arthritis from the spleen, oral Chinese medicine prescription to Jianpihuashi drugs, Huoxue Tongluo drugs, Qufengchushi drugs,drug detoxification,two groups of patients with comprehensive evaluation index and acceptable interventions are long-range correlation,a better effect than that of inflammatory markers associated with traditional Chinese medicine in patients with Furong ointment the simple use of Chinese herbs.
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Objective To observe the clinical efficacy of bone-setting manipulation combined with TCM internal and external treatment for lumbar disc herniation.Methods Totally 240 cases with lumbar disc herniation were selected, and were divided into treatment group and control group according to the random number table method, with 120 cases in each group. Both groups were in bed for rest and did functional exercise. At the same time, modified Mahuang Fuzi XixinDecoction combined withDuhuo JishengDecoction were given, 1 dose per day, three times a day, orally, and were wrapped with a cloth bag dregs, adding 200 mL of vinegar, placed in lumbosacral region afer heating, 2 times a day, external treatment. The treatment group was given bone-setting manipulation combined with TCM internal and external treatment, and the control group was given traction therapy, 1 time for Monday, Wednesday and Friday, 4 weeks as a course of treatment, for continuous 2 courses. The clinical efficacy, visual analogue scale (VAS), Oswestry disability index (ODI), and serum NO content were observed.Results Compared with before the treatment, VAS and ODI score decreased significantly after the first and second courses (P<0.05); The treatment group was significantly lower than the control group at the same time after treatment (P<0.05). The total effective rate was 91.67% (110/120) in treatment group, and 83.33% (100/120) in control group, with statistical significance (Z=-2.103,P=0.036). Compared with before treatment, the NO level of the two groups significantly decreased after treatment, with statistical significance (P<0.05). The NO level in the treatment group was lower than that in the control group after treatment (t=7.843,P=0.041).Conclusion Bone-setting manipulation combined with TCM internal and external treatment have obvious efficacy for lumbar disc herniation, which may be related to down-regulating serum NO level.
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Objective To explore the effects and security of traditional Chinese medicine combined with radiofrequency thermocoagulation in the treatment of lumbar disc herniation.Methods A total of 116 cases with lumbar disc herniation were randomly divided into a treatment group and a control group.The control group (56 cases) was treated with radiofrequency thermocoagulation,while the treatment grouP (60 cases)was treated with Chinese medicine on the basis of the control group.Clinical effect was evaluated by VAS score and JOA score standard analysis before therapy,and 24 hours,1 month,3 month and 6 months after the therapy.Results Thee was no significant differences(x2=0.19,P＞0.05)in VAS and JOA score between the two groups before therapy and 24 hours after the therapy.While after 1 months,3 months and 6 months after the therapy,there was significant difference between the two groups (F value of the control grouP was 168.50、97.80、109.10,F value of the treatment group was 286.50、165.34、75.82,P＜0.01).The therapeutic effects comparison between the two group after 6 months therapy:the recovery rate was 76.7％ and 90.0％ in the control group and the treatment group respectively,showing significant differences (x2=4.568,P＜0.05).Conclusion Chinese medicine combined with radiofrequency thermocoagulation was an effective method for cervical disc herniation.
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Objective:To observe the effect of internal and external use of Chinese material medica with syndrome differentiation on acne vulgaris.Methods:200 patients with acne vulgaris were randomly divided into treatment group(110 cases)and control group(90 cases).Treatment group were given acne facial mask,oral medicine decoction,and the control group were treated with13-cis-Retinoic Acid Capsules,topical Clindamycin Gel,7 weeks for a course of treatment,the clinical effect were compared.Results:The effect on facial lesion in treatment group and control group had significant difference(P