The Influence of Invigorating Spleen and Restoring Virility to the Nerve Peptide Y Level In Brain and Gene Expression / 中国中医药信息杂志

Objective Comparison on the influence of invigorating spleen and restoring virility to the NPY level in brain and gene expression. Method Establishing the spleen insufficiency model of rat by adopting bitter decline diarrhea, wild eating and overworking, to examine the hypothalamus ventral kernel, the hippocampi CA1 area and forehead cortex, the gene expression change of NPY. Result In the model group, the immunoreaction masculine substance of NPY, hypothalamus ventral kernel,hippocampi CA1 area and prefrontal cortex decreases obviously. The immunoreaction masculine substance of NPY on above mentioned parts in the group of invigorating spleen and restoring virility increases distinctly. In the model group, the expression of NPY mRNA on prefronta cortex and hypothalamus ventral kernel decreases obviously, while on the groups of invigorating spleen and restoring virility increases obviously. Conclusion In the model group, the NPY level and gene expression in brain to promote study memory changed. Invigorating spleen and restoring virility may affect study memory by regulating the NPY level and gene expression.

Influence of Invigorating Spleen and Restoring Virility to the SS Level and SSR1 Gene Expression in Brain of Spleen Insufficiency Model of Rat / 中国中医药信息杂志

Objective To compare on the influence of invigorating spleen and restoring virility to the SS level and SSR1 gene expression in brain. Method The spleen insufficiency model of rat was established by adopting bitter decline diarrhea, wild eating and overworking. SS level and SSR1 gene expression change of hypothalamus ventral kernel, hippocampi CA1 area and forehead corte were detected by immunohistochemistry and in situ hybridization techniques. Result In the model group, the immunoreaction masculine substance of SS and SSR1 mRNA, hypothalamus ventral kernel, hippocampi CA1 area and forehead cortex decreased obviously. The immunoreaction masculine substance of SS and SSR1 mRNA on above mentioned parts in the group of invigorating spleen and restoring virility increased distinctly. Conclusion In the model group, the SS level and SSR1 gene expression in brain to promoting study memory ability changed. Invigorating spleen and restoring virility may affect study memory ability by regulating the SS level and SSR1 gene expression.