ABSTRACT
Objective: To study chemical constituents of Argyreia acuta. Methods: The chemical constituents were isolated and purified by silica gel, macroporous resin adsorption, MCI GEL CHP 20P, Sephadex LH-20 chromatography, and preparative liquid chromatography. Their structures were identified by physicochemical properties and spectral analysis. Results: Ten compounds were isolated and identified as ethyl caffeate (1), hispidulin (2), quercetin (3), scopolin (4), paprazine (5), mannitol (6), hesperetin (7), ipalbidinium-4’-O-β-D-(6-O-trans-coumaroyl)-glucoside (8), nepitrin (9), and homoplantaginin (10). Conclusion: Compound 8 is a new compound named argyreiacutine, and compounds 2, 3, 6, 7, 9, and 10 are isolated from A. acuta for the first time.
ABSTRACT
Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining four compounds in Calonyction muricatum.Method An HPLC method was developed as QAMS to determine ipalbidine,ipalbidinium,and ipalbinium in C.muricatum,using ipalbine as the intermal reference substance,the relative correction factor (RCF) of the three components was determined by HPLC with good reproducibility.Their contents in six batches of samples were determined by both extemal standard method and QAMS.Result No significant differences were found in the quantitative results of three alkaloid compounds in six batches of C.muricatum determined by external standard method and QAMS.Conclusion It is feasible and suitable to evaluate the quality ofcurcuma aromatic by QAMS.
ABSTRACT
Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining four compounds in Calonyction muricatum.Method An HPLC method was developed as QAMS to determine ipalbidine,ipalbidinium,and ipalbinium in C.muricatum,using ipalbine as the intermal reference substance,the relative correction factor (RCF) of the three components was determined by HPLC with good reproducibility.Their contents in six batches of samples were determined by both extemal standard method and QAMS.Result No significant differences were found in the quantitative results of three alkaloid compounds in six batches of C.muricatum determined by external standard method and QAMS.Conclusion It is feasible and suitable to evaluate the quality ofcurcuma aromatic by QAMS.