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Objective: To establish an HPLC with charge aerosol detection (HPLC-CAD) method for the determination of kanamy-cin and kanamycin B in kanamycin sulfate injection. Methods: The samples were injected into a Boston Green ODS C18(250 mm× 4. 6 mm, 5 μm) column. The mobile phase was composed of 0. 2 mol·L-1trifluoroacetic acid aqueous solution-methanol (95: 5) , the flow rate was 1. 0 ml·min-1and the column temperature was maintained at 30 ℃. The nebulization temperature for the CAD was maintained at 55℃ and the gas pressure was 56. 4 psi. Results: The linear range of kanamycin was 0. 385-38. 500 μg·ml-1( r=0. 999 9), and the limit of detection was 0. 075μg·ml-1, the limit of quantitation was 0. 154 μg·ml-1, and the average recovery of the method was 100. 97% (n=9). The linear range of kanamycin B was 0. 374-37. 400 μg·ml-1(r=1. 000 0), and the limit of de-tection was 0. 075μg·ml-1, the limit of quantitation was 0. 150 μg·ml-1, and the average recovery of the method was 100. 44% (n=9). Conclusion: The method for the determination of kanamycin and kanamycin B by HPLC-CAD is simple and accurate, the limit of detection is lower than HPLC-ELSD, and the quality of kanamycin sulfate injection can be effectively controlled.
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Objective To evaluate the effect of co -administration of the loop diuretic furosemide (Fur) and kanamycin (KM ) in the rats in order to establish a reliable animal model of sensorineural hearing loss .Methods Thirty -two Sprague-dawley (SD) rats were randomly divided into 4 groups with 8 rats in each group .Rats in group A ,B and C received a single intraperitoneal injection of kanamycin sulfate (KM ,500 mg/kg) ,followed by an-other intraperitoneal injection of furosemide (Fur ,0 .2 ml/kg ) 30 minutes later .While ,rats in control group D re-ceived same doses of normal saline .Auditory brain responses (ABRs) were recorded at 1 ,7 and 14 day after the in-jections ,which were group A ,group B and group C ,respectively .Hair cell loss ,the spiral ganglions and microstruc-ture were observed by immunofluorescence and scanning electron microscopy .Results The ABR thresholds in group A ,B and C were significantly higher than that of in control group D (P0 .05) .Immunofluorescence and scanning electron microscopy showed obvi-ous hair cell loss in the basal turn in each kanamycin groups ,with cilia disarrangement and fusion ,compared to the same areas in animals from the control group .The expression of cleaved caspase -3 significantly increased in each experimental group than that of in the control group(P<0 .05) .Conclusion Co -administration of furosemide and kanamycin intraperitoneally induces profound hearing loss in rats and is an effective method of establishing acute hearing loss model in animal experiments .
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IntroductionNumber of kidney acute and chronic disease is increasing rapidly in the world and becoming the majorcause of death even population employment capacity is invalid. Statistical report of Mongolian Ministryof Health last 5 years statistic kidney disease is in the 3rd of non-contagious disease Synthetic andchemical medicines used for this sort of disease would have side effects in some cases. Plants, animalsand minerals biologically active substances, side effects need to produce new drugs, has attracted theattention of researches.GoalIdentifying pharmacology act of new granule medicine preparation.Material and Methods: The effects of the medicinal substances were investigated on “WISTAR” linesof white rat. Pathological model of nephritis was formed by injected the rats with kanamycin sulfate(Mondodoev.A.J, Lameza.S.B, Bartonov.E.A, 1988). The experimental animals were given any of thenew granular herbal medicine and compared to the rats given Nefromon. After treatment the creatinine,urea acid and MDA in the serum were determined. MDA is identified by an amount of concentration andmethod (Stalinaya. I.D. 1977).ResultCreatinine amount of disease model group of kidney illness created by kanamycin sulfate is comparedwith healthy group animals and 1.64 times, carbamide amount is 4.25 times, rest of the azote’s 2.73are increased and comparing the experiment group creatinine amount is 1.65 creatinine amount is 1.65decreased comparing with disease model group.ConclusionWhen compound ingredients preparation creates experiment animal kanamycin sulfate oxidantdominates, intensify the kidney cell active, decrease the carbamide and creatinine and decrease thekidney cell necrosis.
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OBJECTIVE:To establish a method for content determination of principal agents in kanamycin sulfate eye drops by dye colorimetry.METHODS:On the basis of the spectrometric features of ion pair compound produced in the reaction between kanamycin and bromcresol green in buffer solution with pH value of6.0,absorbance was detected at the wavelength of432nm and content was determined.RESULTS:The detected concentration of Kanamycin showed good linear relationship with its absorbance(r=0.9998)within the range of18.0～72.0?g/ml.The average recovery was99.3%(RSD=1.20%).CONCL_ USION:The established method is rapid,simple and accurate,and it can be used for quick test of this preparation during the production.