ABSTRACT
Objective:To observe expression of stathmin gene in laryngeal squamous cell carcinoma(LSCC) and relation between expression of stathmin gene and occurance and development of LSCC.Method:The expression of the stathmin gene was determined in 35 LSCC of specimens and 18 normal laryngeal tissues(NLT) of specimens by in situ hybridizaion with Digoxigenin labled probe of stathmin mRNA.Result:Expression of stathmin gene was observed in 35 cases of laryneal squamous cell carcinoma tissue (positive rate, 69%) and positive signal was observed in both cytoplasm and nuclear. Among 18 cases of normal tissue, only 6 showed weak positive signal. There was significant difference in expression of stathmin gene between laryneal squamous cell carcinoma tissue and nomal tissue.Conclusion:Expreesion of stathmin gene may play a key role in the pathogenisis and development of laryneal squamous cell carcinoma. It may be a very important biotherapy target in the treatment of laryneal squamous cell carcinoma.
ABSTRACT
Objective To study the correlation between expression of vascular endothelial growth factor(VEGF)and tissue inhibitor of metalloproteinase 2(TIMP-2)and prognosis of patients with laryngeal squamous cell carcinoma.Methods The expression of VEGF and TIMP-2 were measured in 38 specimen of LSCC and 19 specimens of normal laryngeal tissue adjacent to the tumors with EnVision immunohistochemical technique,follow-up for 3~5 years after operation local relapse occurred in four cases,cervical nodular metastasized occurred in fwo cases and pneumonic metastasis occurred one case.Results The expression level of two marks in primary tumors was significantly higher than that in pericancerous tissues(P