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Objective@# To explore the effects of red LED light mediated by the Kelch-like ECH-associated protein 1-nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (KEAP1-NRF2/HO-1) pathway on osteogenic differentiation and oxidative stress damage of human periodontal ligament stem cells (hPDLSCs) induced by high glucose, which provides a basis for the application of red light-emitting diode (LED) light in cell antioxidative damage.@*Methods@#hPDLSCs were identified by flow cytometric analysis, alkaline phosphatase (ALP) staining and Alizarin red-S staining; hPDLSCs were pretreated in a high glucose environment for 48 hours and irradiated with 1, 3, or 5 J/cm2 red LED light. A CCK-8 assay was performed to choose the radiant exposure that had the strongest effect on promoting the cell proliferation rate for subsequent experiments. hPDLSCs were divided into a control group, a high glucose group and a high glucose+light exposure group. ALP staining, ALP activity, Alizarin red-S staining and quantitative calcified nodules were used to detect the osteogenic differentiation of hPDLSCs; qRT-PCR and Western blot were used to detect the gene and protein expression levels of ALP, runt-related transcription factor 2 (RUNX2) and osterix (OSX); the relative mRNA expression levels of antioxidant enzyme-related genes superoxide dismutase 2 (SOD2) and catalase (CAT) in hPDLSCs were detected by qRT-PCR; reactive oxygen species (ROS) levels were detected by fluorescence microscopy and flow cytometry; the tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels in cell supernatants were detected by ELISA; the NRF2-specific inhibitor ML385 was used to inhibit the NRF2 pathway; ALP staining and ALP activity were used to detect the markers of early osteogenic differentiation; qRT-PCR was used to detect the gene expression of ALP, RUNX2 and OSX; and the protein expression levels of KEAP1, NRF2 and HO-1 were detected by Western blot.@*Results @# Identified, and irradiant exposure of 5 J/cm2 was chosen for subsequent experiments. Red LED light irradiation (5 J/cm2) improved the osteogenic differentiation of hPDLSCs induced by high glucose (P<0.05), increased the mRNA and protein levels of ALP, RUNX2 and OSX (P<0.05), upregulated the mRNA expression levels of SOD2 and CAT (P<0.05), reduced the levels of ROS (P<0.05), and reduced TNF-α and IL-1β levels in the cell supernatants (P<0.05). When ML385 was added to inhibit the NRF2 pathway, the ALP activity of cells was decreased (P<0.05); the gene expression levels of ALP, RUNX2 and OSX were downregulated (P<0.05); the protein level of KEAP1 was upregulated (P<0.05); and the protein levels of NRF2 and HO-1 were downregulated (P<0.05)@*Conclusion@#Red LED light may promote the proliferation and osteoblastic differentiation of hPDLSCs induced by high glucose through the KEAP1-NRF2/HO-1 pathway and reduce the oxidative stress damage to hPDLSCs induced by high glucose.
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Objective @#To study the effect of light-emitting diode (LED) red light on the osteogenic/odontogenic differentiation of human dental pulp stem cells (hDPSCs) and its mechanism were discussed. @*Methods@#This study has been reviewed and approved by the Ethics Committee. hDPSCs were cultured by tissue block enzyme digestion. The proliferative capacity of hDPSCs was detected by the CCK-8 at days 1, 3, 5 and 7 under stimulation with 0, 1, 5 and 10 μg/mL lipopolysaccharide (LPS), and the LPS stimulatory concentration was screened. The CG group (mineralization induction), LPS+CG group, and LPS+CG+ (2, 4, 6, 8, and 10 J/cm2) LED red light groups were set. On day 7, alkaline phosphatase (ALP) staining and ALP activity were determined. Relative expression levels of the ALP, osterix (OSX), dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) genes were measured by qRT-PCR. On day 21, alizarin red staining and calcium nodule quantitative determination were performed to screen the best light energy. The LPS+CG group and LPS+CG+LED group (optimal energy) were set up, and the secretion and expression levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were detected by ELISAs on days 1, 3, 5 and 7. The relative expression levels of the extracellular regulated protein kinases 1/2 (ERK1/2), p38, c-Jun N-terminal kinase (JNK), and extracellular regulated protein kinases 5 (ERK5) proteins and their phosphorylated proteins in the MAPK signaling pathway were detected by Western blots. After the pathway was blocked, the relative expression levels of the ALP, OSX, DMP-1, and DSPP proteins after LED red light irradiation on day 7 were detected by Western blots.@*Results@# CCK-8 assays showed that the proliferation of hDPSCs induced by 10 μg/mL LPS was lower than that of the 0, 1, and 5 μg/mL groups on the 5th and 7th days (P<0.05), and 10 μg/mL was selected as the LPS stimulatory concentration in the follow-up experiment. ALP staining, ALP activity, gene expression levels of ALP, OSX, DMP-1 and DSPP and calcium nodule quantification in the LPS+CG+4 J/cm2 group were higher than those in the other treatment groups (P<0.05). 4 J/cm2 LED red light had the strongest ability to promote osteogenic/odontogenic differentiation and was used as the LED light energy density in subsequent experiments. ELISA showed that the secretion and expression levels of TNF-α and IL-1β in the LPS+CG+LED group were lower than those in the LPS+CG group on the 5th and 7th days (P<0.05). Western blot analysis showed that 4 J/cm2 LED red light promoted the expression levels of the p-ERK1/2, p-p38, p-JNK and p-ERK5 proteins. After the MAPK pathway was blocked, the expression levels of the ALP, OSX, DMP-1, and DSPP proteins in the LPS+CG+LED+U0126 (ERK1/2 inhibitor), SP600125 (JNK inhibitor), and BIX02189 (ERK5 inhibitor) groups were lower than those in the LPS+CG+LED group (P<0.001). The protein expression levels of ALP, OSX and DMP-1 in the LPS+CG+LED+SB203580 (p38 inhibitor) group were not significantly different from those in the LPS+CG+LED group (P>0.05).@*Conclusion@#In inflammatory conditions, LED red light promotes osteogenic/odontogenic differentiation of hDPSCs. This effect may be attributed to enhancement of the ERK1/2, JNK, and ERK5 signaling pathways, which reduces the production of the inflammatory cytokines TNF-α and IL-1β.
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Objective:To analyze the willingness-to-pay (WTP) for the treatment of acute facial inflammatory dermatoses with red and yellow light from light-emitting diodes (LEDs), and to evaluate their cost-effectiveness.Methods:A questionnaire survey was conducted on outpatients with or without acute facial inflammatory dermatoses, which mainly manifested as erythema and swelling, in Department of Dermatology, Xiangya Hospital from August 2019 to June 2020. The WTP for the treatment of acute facial inflammatory dermatoses of varying severity with red and yellow light from LEDs were investigated, clinical data on the prevalence of facial skin diseases, previous medical visits and quality of life were collected, and a benefit-cost analysis was conducted. Logistic regression analysis was conducted to investigate factors influencing the patients′ WTP for the LED treatment.Results:The median WTP of the subjects was 200, 300 and 300 RMB yuan for 1-session treatment of three cases of acute facial inflammatory dermatoses of increasing severity assumed to require 1-, 3-, and 5-session LED treatments respectively, and the WTP for 1-session treatment of case 2 and case 3 was higher than the cost of 1-session LED treatment (217 RMB yuan). The subjects with high family incomes (≥ 100 000 RMB yuan/year) were significantly more willing to pay for the treatment than those with low family incomes (< 100 000 RMB yuan/year) (case 1: 40.46% vs. 27.50%, χ2 = 7.00, P = 0.008; case 2: 60.69% vs. 44.5%, χ2 = 9.75, P = 0.002; case 3: 51.20% vs. 48.8%, χ2 = 6.54, P = 0.011), and the subjects who never suffered from facial inflammatory dermatoses were significantly more willing to pay for the treatment than those who ever suffered (case 1: 35.49% vs. 28.82%, χ2 = 1.56, P = 0.212; case 2: 56.10% vs. 42.34%, χ2 = 5.92, P = 0.015; case 3: 68.32% vs. 56.75%, χ2 = 4.58, P = 0.032). No significant difference in the WTP was observed among patients with different facial dermatoses, different degrees of impairment on quality of life, or different treatment experience (all P>0.05) . Conclusion:The cost of red and yellow light from LEDs for the treatment of acute facial inflammatory dermatoses manifesting as erythema and swelling was highly acceptable in this population, and it was worthy of clinical promotion.
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Introduction: the use of light emitting diodes (LED) in domestic and public vias have increased in the last 20 years. In addition, the LED light has been used as a light source for medical applications. Objective: since humans are increasingly exposed to LEDs, there is an urgency to investigate the possible biological effects on tissues caused by this exposure. So, researchers have been focused their investigations in the application of this light in the health field. Material and method: in this review, a search in important databases was performed on the biological effects caused after application of different LED light protocols in in vitro and in vivo studies. Result: although most published papers have shown positive results, some of them reported negative biological effects of light LEDs technology on humans' cells/tissues. Conclusion: therefore, the comprehension of the biological effects caused by light LEDs will provide a better assessment of the risks involved using this technology.
Introdução: o uso de diodos emissores de luz ("LED") em vias domésticas e públicas tem aumentado nos últimos 20 anos. Além disso, a luz LED tem sido usada para aplicações médicas. Objetivo: pelo fato de seres humanos estarem cada vez mais expostos aos LEDs, há urgência em investigar os possíveis efeitos biológicos nos tecidos causados por esta exposição. Assim, pesquisadores têm focado suas investigações no uso desta luz na área da saúde. Material e método: nesta revisão foi realizada uma pesquisa em bancos de dados conceituados sobre os efeitos biológicos causados após aplicação de diferentes protocolos de luz LED em estudos in vitro e in vivo. Resultado: embora a maioria dos artigos publicados tenham mostrado resultados positivos, alguns deles relataram efeitos biológicos negativos da tecnologia de LEDs nas células/tecidos humanos. Conclusão: portanto, a compreensão dos efeitos biológicos causados pela luz LED proporcionará uma melhor avaliação dos riscos envolvidos no uso desta tecnologia.
Subject(s)
Phototherapy , Tissues , In Vitro Techniques , Catchment Area, Health , Cells , Lasers, Semiconductor , Curing Lights, DentalABSTRACT
Carotenoid cleavage dioxygenase (CCD) family is important for production of volatile aromatic compounds and synthesis of plant hormones. To explore the biological functions and gene expression patterns of CsCCD gene family in tea plant, genome-wide identification of CsCCD gene family was performed. The gene structures, conserved motifs, chromosome locations, protein physicochemical properties, evolutionary characteristics, interaction network and cis-acting regulatory elements were predicted and analyzed. Real time-quantitative reverse transcription PCR (RT-qPCR) was used to detect the relative expression level of CsCCD gene family members under different leaf positions and light treatments during processing. A total of 11 CsCCD gene family members, each containing exons ranging from 1 to 11 and introns ranging from 0 to 10, were identified. The average number of amino acids and molecular weight were 519 aa and 57 643.35 Da, respectively. Phylogenetic analysis showed the CsCCD gene family was clustered into 5 major groups (CCD1, CCD4, CCD7, CCD8 and NCED). The CsCCD gene family mainly contained stress response elements, hormone response elements, light response elements and multi-factor response elements, and light response elements was the most abundant (142 elements). Expression analysis showed that the expression levels of CsCCD1 and CsCCD4 in elder leaves were higher than those in younger leaves and stems. With the increase of turning over times, the expression levels of CsCCD1 and CsCCD4 decreased, while supplementary LED light strongly promoted their expression levels in the early stage. The expression level of NCED in younger leaves was higher than that in elder leaves and stems on average, and the expression trend varied in the process of turning over. NCED3 first increased and then decreased, with an expression level 15 times higher than that in fresh leaves. In the late stage of turning over, supplementary LED light significantly promoted its gene expression. In conclusion, CsCCD gene family member expressions were regulated by mechanical force and light. These understandings may help to optimize tea processing techniques and improve tea quality.
Subject(s)
Camellia sinensis/genetics , Gene Expression Regulation, Plant , Phylogeny , Plant Leaves/genetics , Plant Proteins/metabolism , TeaABSTRACT
Objective:To compare efficacy and safety of 308-nm SQ light-emitting diode (LED) light versus 308-nm excimer light in the treatment of facial vitiligo.Methods:Patients with stable facial vitiligo were retrospectively collected from Department of Physical Therapy, Hospital of Dermatology, Chinese Academy of Medical Sciences from June 2018 to June 2020, who received treatment with 308-nm SQ LED light (LED group) or 308-nm excimer light (excimer light group). The treatment was performed once or twice a week, and patients who had received more than 8 sessions of treatment were included in the analysis of efficacy and safety. Statistical analysis was carried out by using chi-square test.Results:Totally, 68 patients with 90 lesions were enrolled into the LED group, including 36 males and 32 females, aged 25.01 ± 13.37 years; 20 patients with 28 lesions were enrolled into the excimer light group, including 13 males and 7 females, aged 27.15 ± 14.30 years. After 8 and 16 sessions of treatment, there was no significant difference in the response rate between the LED group (23.33%, 46.67%, respectively) and excimer light group (14.29%, 46.43%, χ2 = 1.05, < 0.001, respectively, both P > 0.05). During the treatment, 36 (52.94%) patients in the LED group developed persistent erythema, 17 (85%) in the excimer light group developed persistent erythema or blisters. The incidence of adverse reactions was significantly lower in the LED group than in the excimer light group ( χ2 = 16.43, P < 0.001) . Conclusion:Compared with the 308-nm excimer light, the 308-nm SQ LED light showed similar effect but higher safety for the treatment of facial vitiligo.
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ABSTRACT: Light is one of the factors that influence the germination and initial establishment of orchids under in vitro cultivation. This study evaluated the effect of different light sources on these stages in in vitro cultivation of Schomburgkia crispa Lindl. After sowing in an aseptic environment, we stored the cultures in a screened greenhouse (natural light) or in a growth room with the following light sources: 3,000 K yellow LED; 6,500 K white LED [1]; 6,500 K white LED [2]; or 6,500 K white fluorescent lamp (control). We assessed germination percentage and initial seedling establishment at 45 and 90 days after sowing. Light did not influence the germination of S. crispa. However, the use of 3,000 K LED provided a faster initial establishment of S. crispa when compared to the other light sources, also presenting lower seedling mortality. Thus, the light source 3,000 K LED is a potential substitute for the 6,500 K fluorescent lamps and LEDs used in growth rooms in in vitro culture laboratories.
RESUMO: A luz é um dos fatores que influenciam a germinação e o estabelecimento inicial no cultivo in vitro de orquídeas. Assim, objetivou-se avaliar o efeito de diferentes fontes de luz na germinação e no estabelecimento inicial in vitro de Schomburgkia crispa Lindl. Após semeadura em ambiente asséptico, as culturas foram acondicionadas em viveiro telado (luz natural) ou em sala de crescimento nas seguintes fontes luminosas: LEDs amarelo 3.000 K, branco 6.500 K [1], branco 6.500 K [2] ou lâmpada fluorescente branca 6.500 K (controle). Aos 45 e 90 dias após a semeadura foi avaliada a porcentagem de germinação e o estabelecimento inicial dos propágulos. A luz não influenciou a germinação das sementes de S. crispa, mas a utilização do LED 3.000 K proporcionou estabelecimento inicial de S. crispa em menor período e com menor mortalidade das plântulas, quando comparado com as demais fontes de luz utilizadas. A fonte de luz LED 3.000 K pode ser indicada como um substituto potencial para as lâmpadas fluorescentes e LEDs 6.500 K, utilizadas em salas de crescimento em laboratórios de cultivo in vitro.
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Background: The most commonly used light sources in the conventional phototherapy units are compact fluorescent lamp (CFL), halogen spotlights and fiberoptic blankets. Recently light emitting diodes (LED) has emerged as better light source for phototherapy and almost replacing all the available conventional light sources. Comparative studies on the efficacy of LED versus conventional phototherapy are limited from India. That is why; this study was undertaken.Methods: 48 neonates of ≥35 weeks gestational age with hyperbilirubinemia were participated in this study. Among them, 24 neonates received conventional phototherapy and rest of them received LED phototherapy. The rate of fall of bilirubin levels at 6 hours and at completion along with total duration of phototherapy in both groups was measured. Results analysed by standard statistical methods.Results: LED phototherapy units showed higher rate of fall in bilirubin at six hrs (LED group: 0.38±0.05 mg/dl/hr versus conventional group: 0.30±0.04 mg/dl/hr, p<0.05) and after completion of therapy (LED group: 0.32±0.03 mg/dl/hr versus conventional group: 0.26±0.03 mg/dl/hr, p<0.05) compared to conventional group. Significant difference was documented in total duration of phototherapy in LED group (30.8±1.8 hours) when compared to conventional group (34.6±0.7 hours). None of the neonate showed phototherapy failure. Side effects were minimal and comparable in both the groups.Conclusions: The LED phototherapy units are more efficacious in terms of higher rate of fall of bilirubin levels and lesser duration of phototherapy compared to conventional phototherapy units.
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Objective: This study evaluates the shear bond strength (SBS) and Vickers micro-hardness (VHN) of the orthodontic adhesive resin Transbond XT using thermocycled samples. The study also measures the degree of cure by poliwave light curing unit Valo. Materials and Methods: 135 brackets were bonded for the SBS test, and 90 disc-shaped orthodontic adhesive resins were prepared for the VHN test. The measurements were taken either immediately or after 500 or 10.0000 thermocycling. The SBS and VHN test data were statistically evaluated using a Two-way ANOVA and Tukey multiple comparison tests. The degree of conversion (DC) was measured by FTIR. Results: The 10-sec polymerized control group (57.08) and the 10.000 termocycled samples (55.96) had the highest VHN scores. On the other hand, the 3 second polymerized and the 10.000 termocycled samples (39.22) had the lowest VHN scores. There was no significant difference in the bracket SBS values between the termocycled and immediate groups. An evaluation of the bond failure surfaces revealed that the ARI scores did not differ between the immediate and the thermo-cycled groups according to the power modes. The FTIR results revealed that the lowest DC was seen in the 3 second light-cured sample. Conclusion: TransbondXT provided sufficient bond strength, microhardness and DC under in vitro bracket bonding conditions after 3 and 10 s of Poliwave LED curing. However, low mean values were seen in the groups that received Poliwave LED curing for only 3 s (AU)
Objetivo: Este estudo avalia a resistência ao cisalhamento (SBS) e a microdureza Vickers (VHN) da resina adesiva ortodôntica Transbond XT utilizando amostras termocicladas. O estudo também mede o grau de cura pela unidade de fotopolimerização Valo poliwave. Materiais e Métodos: 135 braquetes foram colados para o teste SBS e 90 discos de resina adesiva ortodôntica foram preparados para o teste VHN. As medições foram feitas imediatamente ou após 500 ou 10.000 ciclos de termociclagem. Os dados dos testes SBS e VHN foram avaliados estatisticamente usando ANOVA 2 fatores e teste de comparação múltipla de Tukey. O grau de conversão (DC) foi medido por FTIR. Resultados: O grupo controle polimerizado por 10segundos (57,08) ciclados por 10.000 ciclos (55,96) tiveram os valores VHN mais altos. Por outro lado, as amostras polimerizadas de 3 segundos e 10.000 ciclos (39,22) os valores de VHN mais baixos. Não houve diferença significativa nos valores de SBS do braquete entre os grupos termociclados e imediato. Uma avaliação das superfícies de falha de adesão revelou que os escores do ARI não diferiram entre os grupos imediato e termociclados de acordo com as densidades de potência. Os resultados do FTIR revelaram que o menor DC foi observado nas amostras fotopolimerizadas de 3 segundos. Conclusão: Transbond XT forneceu suficiente resistência de união, microdureza e DC sob condições de colagem de braquetes in vitro após 3 e 10 s de polimerização em LED Poliwave. No entanto, valores médios baixos foram observados nos grupos que receberam a cura do LED Poliwave por apenas 3 s (AU)
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Orthodontics , Spectroscopy, Fourier Transform Infrared , Shear Strength , Lasers, SemiconductorABSTRACT
Abstract The aim of this study was to evaluate the in vivo response of red light-emitting diode (LED) on acute lung injury (ALI) in a sepsis model in rats. Twenty rats were randomly allocated into two experimental groups (n=10): Control Sepsis Group (CS); sepsis and red LED group (SRL). The anterior region of the trachea and ventral regions of the chest (below the ribs), bilaterally were irradiated daily for two consecutive days, starting immediately after the surgery using red (630 nm) LED. The histological results showed that in red LED treated group presented a modulation of the lung inflammatory process, less intense alveolar septum thickening and decrease of the inflammatory cells. Moreover, LED significantly reduced the lung injury score and increased interleukin type 10 (IL-10) protein expression compared SG. These results suggest that LED was efficient in attenuating ALI in a sepsis model in rats by reducing inflammatory cells into lung tissue and enhancing the anti-inflammatory cytokine production.
Subject(s)
Animals , Male , Rats , Sepsis/therapy , Low-Level Light Therapy , Lasers, Semiconductor , Acute Lung Injury/therapy , Biomarkers , Rats, Wistar , Disease Models, AnimalABSTRACT
Objective: The aim of this in vitro study was to evaluate the efficacy of photodynamic inactivation (PDI) with erythrosine (E), using a light-emitting diode (LED) on planktonic cultures of Streptococcus mutans. Material and Methods: A Streptococcus mutans strain (UA 159) was used to prepare the suspensions containing 107 cells/mL, which was tested under different experimental conditions: a) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); b) LED irradiation only (P-L+); c) treatment with erythrosine only (E+L-); and d) no LED irradiation or photosensitizer (P) treatment, which served as a control group (P-L-). After treatment, strains were seeded onto MSBS agar for determination of the number of colony-forming units (CFU/mL). Results: The results were submitted to analysis of variance and the Tukey test (p < 0.05). No reduction in the number of CFU/mL was observed in the treatment group with erythrosine (E+L+) when compared to the control (P-L-). Conclusion: PDI using erythrosine did not reduce the number of CFUs per millimeter within the parameters in this study. (AU)
Objetivo: o objetivo deste estudo in vitro foi avaliar a eficácia da inativação fotodinâmica (PDI) com a eritrosina (E), usando diodo de emissão de luz azul (LED) em culturas planctônicas de Streptococcus mutans. Material e métodos: a cepa de Streptococcus mutans (UA 159) foi usada para o preparo das suspensões padrões contendo 107 células/mL, as quais foram testadas em diferentes condições experimentais a) irradiação com LED em presença da eritrosina como fotossensibilizador (E+L+); b) irradiação com LED apenas (F-L+); c) tratamento com eritrosina apenas (E+L-); e d) tratamento sem irradiação com LED ou fotossensibilizador (F), que serviu como grupo controle (F-L-). Após o tratamento, as cepas foram semeadas em ágar MSBS para determinação do número de unidades formadoras de colônias (UFC/mL). Resultados: os resultados foram submetidos à análise de variância e teste de Tukey (p < 0.05). Não foi observada redução no número de UFC/mL no grupo de tratamento com eritrosina (E+L+) quando comparado ao grupo controle (F-L-). Conclusão: a PDI usando etritrosina e LED não reduziu o número de UFCs por milímetro com os parâmetros utilizados neste estudo.(AU)
Subject(s)
Streptococcus mutans , Photosensitizing Agents , Dental Caries , ErythrosineABSTRACT
Background: Due to low sensitivity and inability to detect drug resistance, smear microscopy limits its impact on TB control. Culture methods and drug susceptibility testing is complex, time consuming, and takes around 6-8 weeks. A new diagnostic test, cartridge based nucleic acid amplification test (CBNAAT) was developed based on real-time polymerase chain reaction (RT PCR). Objective of this study to compare the results of CBNAAT for diagnosis of pulmonary tuberculosis with LED fluorescent microscopy and sputum culture.Methods: A cross-sectional study was conducted in the department of Chest and TB, CIMS, Bilaspur. Each Sputum sample of presumptive TB patients were tested with CBNAAT, sputum smear microscopy by light emitting diode (LED) fluorescent microscopy (FM) and solid and liquid culture for diagnosis of Tuberculosis. Results of CBNAAT, Fluorescent Microscopy and Culture for detection of Mycobacterium Tuberculosis were compared.Results: The sensitivity and specificity for CBNAAT were 97% and 100% respectively; while that for Fluorescent microscopy were 70% and 100% respectively. The positive and negative predictive value for CBNAAT was 100% and 96% respectively. The positive and negative predictive value for Fluorescent microscopy was 100% and 73% respectively.Conclusion: CBNAAT is having high sensitivity and specificity for diagnosis of pulmonary tuberculosis. It should be routinely used under national health programme to detect a tuberculosis case efficiently.
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RESUMO As lâmpadas fluorescentes (LFs) podem causar sérios problemas ambientais e à saúde humana. Tal fato se deve à presença de mercúrio em sua constituição e, quando descartadas inadequadamente, podem ocasionar a contaminação do solo, do ar e da água. Já as lâmpadas light emitting diode (LED), além de mais econômicas e com maior vida útil que as fluorescentes, proporcionam maior luminosidade e, ao serem substituídas, não contaminam o meio ambiente. O objetivo deste estudo foi avaliar a substituição das LFs pelas lâmpadas LED na Universidade Federal de Uberlândia (UFU), em Uberlândia, Minas Gerais, analisando seu impacto ambiental e a viabilidade econômica e financeira. Concluiu-se que as lâmpadas LED se sobressaem nos aspectos de manutenibilidade, durabilidade, eficiência energética, economia e menores impactos ambientais, podendo ser até duas vezes mais eficientes que as LFs, além de não gerarem resíduos tóxicos. Diante desse cenário, constatou-se a viabilidade econômico-financeira para a substituição das LFs por LED na UFU, pois, considerando-se uma amostra de 1.000 LFs, projetou-se uma economia entre 55,75 e 80,78% ao completar 13 anos e um retorno do montante gasto ao final do primeiro ano. Não obstante, o que mais se destacou nessa experiência foi a importância da substituição do ponto de vista ambiental, por contemplar ações de sustentabilidade, principalmente em uma instituição de ensino.
ABSTRACT Fluorescent lamps (FLs) can cause serious environmental and human health problems. This is due to the presence of mercury in its constitution and that, when discarded improperly, they can cause soil, air and water contamination. Light emitting diode (LED) lamps, in addition to being more economic and with a life longer than fluorescent lamps', they provide greater brightness and, when replaced, do not contaminate the environment. The objective of this study was to evaluate the substitution of FLs with LED at the Federal University of Uberlândia (UFU), in Uberlândia, Minas Gerais (Brazil), by analyzing their environmental impact and economic and financial viability. It was possible to conclude that LED lamps stand out in terms of maintainability, durability, energy efficiency, economy and lower environmental impacts, being up to twice as efficient as FLs and not generating toxic waste. In this scenario, the economic and financial viability for FL's substitution in UFU was verified, since that if a sample of 1,000 FLs was considered, the projected economy was between 55.75 and 80.78% by the end of 13 years and the return of the amount spent at the end of the first year. However, what stood out most in this experiment was the importance of the replacement from the environmental point of view, since it primarily contemplates sustainability actions in an educational institution.
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Background: The aim of the study was to evaluate whether light-emitting diode (LED) phototherapy is as efficacious as compact fluorescent tube (CFT) phototherapy for the treatment of non-hemolytic jaundice in healthy term and late preterm neonates.Methods: Study design was open label randomized controlled trial conducted at tertiary care NICU. Healthy term and late preterm neonates with non-haemolytic jaundice included in the present study. Intervention was double-surface LED or CFT phototherapy. Primary outcome variable was duration of phototherapy.Results: A total of 60 neonates were randomized to receive LED (n=30) or CFT (n=30) phototherapy. The baseline demographic and biochemical variables were similar in the two groups. The median duration of phototherapy (Mean'SD) in the two groups was comparable (26.7'7.0) h vs (24.8'6.05) h, P=0.0.241). The rate of fall of serum total bilirubin (STB) during phototherapy in initial 6 hours was significantly more LED group (n=30), 3.43'0.65 versus (n=30) 2.22'0.55 with P-value of <0.001.Conclusions: LED and CFT phototherapy units were equally efficacious in the management of non-haemolytic hyperbilirubinemia in healthy term and late-preterm neonates.' Side effects were rare, comparable in the two groups and included only rash.
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Introduction: Over the years, the demand for esthetic dentistry has grown dramatically and there has been a rapid developmentof new adhesive restorative materials with nanotechnology that can restore the color and characteristics of natural tooth. Topolymerize these materials, light-curing dental materials extensively used are quartz-tungsten-halogen (QTH) and light-emittingdiode (LED)-curing units. Literature search revealed that depth of cure and flexural strength are the most important propertiesof composite resin materials, relevant to the clinical technique of incremental packing and curing.Purpose: The objectives of the present study were to evaluate and compare the depth of cure and flexural strength of ananohybrid composite resin.Materials and Methods: Two light-curing units were selected for this study: QTH (Bonart, Unicorn) and LED (Ivoclar Vivadent,Bluephase® N). The depth of cure was evaluated with scraping technique using digital caliper and flexural strength was evaluatedusing universal testing machine with a crosshead speed of 1 mm/min.Results: Descriptive statistics was employed to measure the mean and standard deviation of the depth of cure and flexuralstrength. Unpaired “t”-test was used to compare the study variables. Statistical significance was fixed at ≤0.05 and LED-curingunit showed significantly greater depth of cure and flexural strength when compared to QTH curing unit.Conclusion: Curing effectiveness of resin composite is dependent on the light-curing unit.
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BACKGROUND: The light-emitting diode (LED) curing light used is presumed to be safe. However, the scientific basis for this is unclear, and the safety of LED curing light is still controversial. The purpose of this study was to investigate the effect of LED curing light irradiation according to the conditions applied for the polymerization of composite resins in dental clinic on the cell viability and inflammatory response in Raw264.7 macrophages and to confirm the stability of LED curing light. METHODS: Cell viability and cell morphology of Raw264.7 macrophages treated with 100 ng/ml of lipopolysaccharide (LPS) or/and LED curing light with a wavelength of 440~490 nm for 20 seconds were confirmed by methylthiazolydiphenyl-tetrazolium bromide assay and microscopic observation. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) was confirmed by NO assay and PGE2 enzyme-linked immunosorbent assay kit. Expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in total RNA and protein was confirmed by reverse transcription polymerase chain reaction and Western blot analysis. RESULTS: The LED curing light did not affect the viability and morphology of normal Raw264.7 cells but affected the cell viability and induced cytotoxicity in the inflammation-induced Raw264.7 cells by LPS. The irradiation of the LED curing light did not progress to the inflammatory state in the inflammation-induced Raw264.7 macrophage. However, LED curing light irradiation in normal Raw264.7 cells induced an increase in NO and PGE2 production and mRNA and protein expression of IL-1β and TNF-α, indicating that it is possible to induce the inflammatory state. CONCLUSION: The irradiation of LED curing light in RAW264.7 macrophage may induce an excessive inflammatory reaction and damage oral tissues. Therefore, it is necessary to limit the long-term irradiation which is inappropriate when applying LED curing light in a dental clinic.
Subject(s)
Blotting, Western , Cell Survival , Composite Resins , Dental Clinics , Dinoprostone , Enzyme-Linked Immunosorbent Assay , Interleukins , Macrophages , Nitric Oxide , Polymerase Chain Reaction , Polymerization , Polymers , Reverse Transcription , RNA , RNA, Messenger , Tumor Necrosis Factor-alphaABSTRACT
OBJETIVOS: Apresentar a transformada wavelet como uma ferramenta alternativa para o processamento dos sinais oriundos da eletromiografia quando utilizada na avaliação da atividade elétrica do músculo masseter de mulheres com disfunção temporomandibular. MÉTODOS: Cinco voluntárias com disfunção temporomandibular passaram por quatro sessões de terapia com diodo emissor de luz (LED, light emitting diode). A eletromiografia do músculo masseter foi realizada bilateralmente antes e após o tratamento, empregando um eletromiógrafo de dois canais. Na análise dos sinais eletromiográficos foi empregada a transformada wavelet na função Morlet. RESULTADOS: Nos escalogramas, observou-se a diminuição da ativação das fibras de alta frequência no protocolo de repouso e seu aumento no protocolo de movimento isométrico. Na análise baseada no sistema de cores RGB, foi possível observar que no protocolo de repouso do músculo masseter direito houve redução dos momentos de máxima intensidades de energia em 82% para frequências de 256-512Hz e em 42% para frequências acima de 512Hz. No músculo masseter esquerdo a redução foi de 42% na banda de frequências de 256-512Hz. CONCLUSÕES: A análise pela transformada wavelet permitiu identificar fatores fisiológicos relacionados não somente à ativação do músculo masseter, mas também à intensidade e à relação tempo/frequência, assim como os principais tipos de fibras ativadas durante os protocolos antes e após a terapia LED em pacientes com disfunção temporomandibular
AIMS: To present the wavelet transform as an alternative tool in the evaluation of the masseter muscle electrical activity in women with temporomandibular disorder after therapy with Light Emitting Diode (LED). METHODS: Five volunteers with temporomandibular disorder underwent four sessions of LED therapy. Electromyography of the masseter muscle was performed bilaterally before and after treatment. For analysing the electromyographic signals, the wavelet transform was applied in the Morlet function. RESULTS: In the scalogram, a decrease in the activation of the high-frequency fibers in the rest protocol and its increase in the isometric movement protocol were observed. In the analysis based on the RGB color system, we observed that in the right masseter muscle resting protocol, the moments of maximum energy intensities were reduced by 82% for frequencies of 256-512Hz and by 42% for frequencies above 512Hz. In the left masseter muscle the reduction was 42% in the frequency band of 256-512Hz. CONCLUSIONS: Analysis by the wavelet transform allowed identification of physiological factors related not only to the activation of the masseter muscle, but also to the intensity and time / frequency relationship, as well as the main types of fibers activated during the protocols before and after LED therapy in patients with dysfunction temporomandibular.
Subject(s)
Electromyography , Low-Level Light Therapy , Temporomandibular Joint Dysfunction Syndrome/therapyABSTRACT
In a photodynamic therapy, the difference of antibacterial capacity was compared according to the type of source of light when the same quantity of energy is irradiated.After S. mutans is formed in planktonic state and biofilm state, erythrosine diluted to 40 µM was treated for 3 minutes, and as the type of light source, Halogen, LED, and Plasma arc were used, which were irradiated for 30 seconds, 15 seconds and 9.5 seconds, respectively.After the completion of the experiment, CFU of each experiment arm was measured to compare the photodynamic therapeutic effects according to each condition.The CFU of each experiment arm had no statistically significant difference.Under the same quantity of energy, the photodynamic therapeutic effect can be said to be the same regardless of types of light source, which is a useful result in the clinical field with various light irradiators.
Subject(s)
Arm , Biofilms , Erythrosine , Photochemotherapy , Plankton , Plasma , Streptococcus mutans , Streptococcus , Therapeutic UsesABSTRACT
Under different red (R):blue (B) photon flux ratios, the growth performance of rapeseed (Brassica napus L.) is significantly different. Rapeseed under high R ratios shows shade response, while under high B ratios it shows sun-type morphology. Rapeseed under monochromatic red or blue light is seriously stressed. Transcriptomic and proteomic methods were used to analyze the metabolic pathway change of rapeseed (cv. "Zhongshuang 11") leaves under different R:B photon flux ratios (including 100R:0B%, 75R:25B%, 25R:75B%, and 0R:100B%), based on digital gene expression (DGE) and two-dimensional gel electrophoresis (2-DE). For DGE analysis, 2054 differentially expressed transcripts (|log2(fold change)|≥1, q<0.005) were detected among the treatments. High R ratios (100R:0B% and 75R:25B%) enhanced the expression of cellular structural components, mainly the cell wall and cell membrane. These components participated in plant epidermis development and anatomical structure morphogenesis. This might be related to the shade response induced by red light. High B ratios (25R:75B% and 0R:100B%) promoted the expression of chloroplast-related components, which might be involved in the formation of sun-type chloroplast induced by blue light. For 2-DE analysis, 37 protein spots showed more than a 2-fold difference in expression among the treatments. Monochromatic light (ML; 100R:0B% and 0R:100B%) stimulated accumulation of proteins associated with antioxidation, photosystem II (PSII), DNA and ribosome repairs, while compound light (CL; 75R:25B% and 25R:75B%) accelerated accumulation of proteins associated with carbohydrate, nucleic acid, amino acid, vitamin, and xanthophyll metabolisms. These findings can be useful in understanding the response mechanisms of rapeseed leaves to different R:B photon flux ratios.
Subject(s)
Brassica napus/radiation effects , Brassica rapa/radiation effects , Carbon/chemistry , Chloroplasts/radiation effects , Computational Biology , Electrophoresis, Gel, Two-Dimensional , Gene Expression Regulation, Plant/radiation effects , Image Processing, Computer-Assisted , Light , Mass Spectrometry , Metabolic Networks and Pathways , Nitrogen/chemistry , Photons , Photosystem II Protein Complex/genetics , Plant Leaves/radiation effects , Plant Proteins/genetics , Proteome , Ribosomes , Transcription, Genetic , TranscriptomeABSTRACT
BACKGROUND Tuberculosis (TB) remains one of the leading causes of morbidity and mortality worldwide. The primary method for controlling TB is the rapid and accurate identification of infected individuals. Immune response exploitation represents one of the main methods used for early TB diagnosis; however, few studies have reported that whole blood originating from TB-infected patients gels faster in the presence of aldehyde than blood originating from healthy subjects, which is the focus of the current study. OBJECTIVES The study objectives are to determine the diagnostic value of a glutaraldehyde test (GT) in pulmonary tuberculosis (PTB) and extra-pulmonary tuberculosis (EPTB) and to assess its performance compared with light-emitting diode fluorescence microscopy (LED-FM). MATERIALS AND METHODS This study included 272 specimens (176 suspected PTB specimens and 96 suspected EPTB specimens). Of the 272 patients, 98 patients had TB infection confirmed by culture (64 PTB cases and 34 EPTB cases), and 174 patients had no TB infection. The gold standard technique (culture) was used as reference to verify the GT's performance. RESULTS The GT showed a high sensitivity (96.9%) and specificity (82.1%) for PTB with a good positive predictive value (PPV = 75.6%) and negative predictive value (NPV = 97.9%). For EPTB, the GT showed a sensitivity of 91.2% and a specificity of 77.4%, with PPV = 68.9% and NPV = 94.1%. LED-FM had lower sensitivities for PTB (65.6%) and EPTB (42.1%) and an excellent specificity of 100%, with PPV = 100% and NPV = 100%. CONCLUSION We concluded that GT is rapid, easy, simple and cost-effective and does not require qualified personnel with a specific background or sophisticated equipment like molecular biology or mycobacterium-specific genotyping techniques. These qualities make the GT attractive for use in low- and high-income countries in addition to other conventional methods, particularly culture, which continues to be the gold standard.