ABSTRACT
Objective To establish animal models facilitating observation of the process of epithelial-mesenchymal transition between liver cirrhosis and liver cancer. Methods Liver cirrhosis and liver cancer models were established in mouse and subsequently assessed using HE and immunohistochemical staining. Results The success rate of the liver cirrhosis model was 100％ and the fatality rate was 30％. The success rate of the liver cancer model was also 100％ and the fatality rate was 40％. In the liver cirrhosis and liver cancer models,the positive expression rates were,respectively,46. 7％ and 7. 1％ for E-cadherin,40. 0％ and 7. 1％ for β3-integrin,13. 6％ and 40. 0％ for vimentin,and 20. 0％ and 57. 1％ for fibronectin. Conclusion In mouse models of liver cirrhosis and liver cancer,low expression levels of E-cadherin and β3-integrin in addition to high expression levels of fibronectin and vimentin are related to the epithelial-mesenchymal transition between liver cancer and cirrhosis,and may be important indicators for clinical detection.
ABSTRACT
Objective To investigate MELD and Child-Pugh in the patients with decompensated liver cirrhosis.Methods 41 cases of death and 71 survivors were graded with MELD and Child-Pugh and compared.Results The deaths' MELD and Child-Pugh score was (17.93?8.22 )and (10.07?1.84),the survivors' was (11.18?6.54 ) and (8.04?2.09)(P=0.000).Among the deaths and survivors,MELD≤9 was 14.63% and 42.25%(P =0.003),MELD 20-29 was 26.83% and 8.45%(P=0.009),Child A was 21.95% and 56.34%(P=0.000),Child C was 29.27% and 5.63%(P=0.001).Conclusion The deaths' MELD and Child-Pugh score is higher than the survivors'.The MELD score can act as a disease severity and prognosis index for patients with decompensated liver cirrhosis.