ABSTRACT
Objective To investigate the methods for the detection of serum samples with low-concentration HBsAg and its clin-ical value.Methods Serum samples with HBsAg weak positive were measured by ELISA or using Roch cobas e601 were collected from the clinical laboratory.The samples with low-concentration HBsAg were confirmed by neutralization assay by using Lizhu ELISA reagent Kit or Roch cobas e601 analyzer.at the same time,ALT/AST concentrations and HBV DNA copies were also deter-mined.Results Among 53 samples that showed weak positive reaction by using Kehua reagent kit,37 samples were positive in neu-tralization test,while the other 16 samples were negative.Among 18 samples that showed weak positive reaction tested by using Roch cobas e601 analyzer,13 samples showed positive in neutralization test,and the other 5 samples showed negative.Conclusion The results of HBsAg test varies in different detection System.The clinical significance of samples with low-concentration HBsAg needs further study.
ABSTRACT
enxertia cutânea é uma das técnicas mais comuns usadas na cirurgia plástica reparadora. Esta técnica é usada para cobertura de defeitos da pele resultantes de uma ampla variedade de causas. Diversos métodos para melhorar a viabilidade desses transplantes podem ser utilizados. Os agentes físicos podem ser importantes recursos no processo de integração do enxerto de pele; o objetivo deste estudo foi revisar estes agentes. A revisão da literatura foi realizada por meio das bases de dados Pubmed, Lilacs e SciELO, no período de 1970 a 2009. Os agentes físicos ainda são pouco utilizados, mas importantes na integração, função e estética dos enxertos de pele. Entre os artigos pesquisados, todos obtiveram resultados satisfatórios com a utilização dos diferentes agentes físicos na viabilidade do enxerto de pele.
Skin graft is one of the most common techniques used in reconstructive surgery. This technique is used for covering defects on the skin resulting from an ample variety of causes. Many methods to improve the viability of these transplants can be used. Physical agents can be important resources in the process of skin graft integration. The objective of this study was to review these physical agents. The literature was sought in the databases Pubmed, Lilacs and Scielo, using articles published between 1970 and 2009. The use of physical agents is still uncommon, but they are important in the integration, function and appearance of skin grafts. All reviewed articles reported satisfactory results in the use of different physical agents to improve the viability of skin grafts.