ABSTRACT
Objective To study whether L-cysteine and lysolecithin (16:0) could be as a serum markers in the detection of OVC for overcoming the OVC defects of early detection .Methods Totally 142 cases of healthy check -up patients ( control group ) 100 cases from First Affiliated Hospital of Wenzhou Medical University in January 2012 to January 2014 were extracted patient specimens .These pa-tient specimens were used to detect by MALDI -TOF-MS mass spectrometer and obtain the peak m/z by the method of principal compo-nent analysis for screening expression difference between the two groups in the metabolites and the correlation between metabolites and pathological grade.Results The most difference substance between two groups were 184.05 and 496.30m/z which identified as LPC (16:0) and HCA;On HCA average level and detection rate , OVC group was significantly higher than control (P<0.01); On 184.05 and 496.30m/z peak area, control group was significantly higher than OVC group (P<0.01);HCA positively was correlated with patho-logic grade (P<0.05);184.05 and 496.30 m/z peak area were negative correlation with pathologic grade (P<0.05).Conclusion L-cysteine and lysolecithin (16:0) mechanism in the pathogenesis of OVC is unclear , but it can be used for the detection of serum OVC markers.
ABSTRACT
OBJECTIVE: To determine lysolecithin and in vitro hemolysis of propofol nano-injection. METHODS: An HPLC method was established for determination of lysolecithin. The sample was analyzed with a Platisil C18 column (4.6 mm × 250 mm, 5 μm) at 30°C. Mobil phase A was acetonitrile-water (9:1). Mobil phase B was 0.2% phosphoric acid aqueous solution (pH adjusted to 2.5 with triethylamine). Gradient elution program was used as follows; 0 min, 60% A; 15 min, 60% A; 16 min, 90% A; 22 min, 90% A; 23 min, 60% A; 35 min, 60% A. The flow rate was 1.2 mL ·min-1. The detection wave length was set at 210 nm. Spectrophotometric method was used to determine hemolysis. RESULTS: The linear range of the calibration curve for lysolecithin was 19. 8-198.0 μg · mL-1 (r=0.9998). The average recovery was 102.0% and RSD was 0.63% (n=9). The lysolecithin contents of three batches of samples were 0.313, 0.273 and 0.318 mg · mL-1, respectively. The hemolysis rates were 2.70%, 3.37% and 3.04%, respectively. CONCLUSION: The HPLC method can be used for the determination of lysolecithin in propofol nano-injection. The hemolysis rates of three batches of samples were less than 5%.
ABSTRACT
Flow cytometry, a useful tool for measuring DNA content and cell differentiation as expressed by cell surface markers, is utilized to measure multiple antigens, especially surface antigen, intracellular oncoprotein, and DNA content, simultaneously. For this simultaneous detection, several methods off ixation and permeabilization have been used with limited values. In this study, 20 ng/ml of lysolecithin in 1% paraformaldehyde solution was utilized for fixation and permeabilization of cultured promyelocytic leukemic cells(HL 60). The cells were first stained with phycoerythrin (PE)-conjugated monoclonal antibody to the cell surface My 7 antigen and then were fixed and permeabilized with 20 ng/ml of lysolecithin in 1% partormaldehyde solution. After incubation, the fixed and permeabilized cells were stained with monoclonal antibody to intracellular c-myc antigen, which were followed by fluorescein isothiocyanate (FITC)-conjugated secondary antibody. The c-myc stained cells were finally stained for DNA content with 7-amino-actinomycin D(7-AAD). This procedure permits excellent staining for intracellular oncoproteins and preservation of surface antigens with relatively low cofficients of variation (CV) for the G0G1 peak of the DNA histograms and suggests that the sequential staining procedure of surface antigen, intracellular antigen, and DNA content will be extended for the study of correlations with cellular differentiation, expression of oncoproteins, and cell cycle analysis in the cells which are obtained from human malignant diseases using a 488 nm single laser flow cytometry.