ABSTRACT
In the present study, total methanol extracts prepared from Alpinia katsumadai showed significant protective effects against the oxidative stress induced by hydrogen peroxide, UV-C or gamma-ray irradiation. These protective effects were substantially increased by treatment with 20~100 microg/ml of the extract. The A. katsumadai total methanol preparation was further fractionated into n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions. Among these five fractions, the ethylacetate and butanol fractions of A. katsumadai showed the strongest protective effects against oxidative stress induced by UV-C and gamma-ray irradiation. These fractions also showed high DPPH radical scavenging and lipid peroxidation inhibitory activities. In addition, both fractions displayed cell proliferation activation effects, as evidenced by significant increases in colony formation. Our current data thus suggest that the mechanisms underlying the protective effects of A. katsumadai against oxidative damage may include radical scavenging, protection against cell membrane damage and stimulation of cell proliferation.
Subject(s)
1-Butanol , Alpinia , Cell Membrane , Cell Proliferation , Hexanes , Hydrogen Peroxide , Lipid Peroxidation , Methanol , Methylene Chloride , Oxidative Stress , Reactive Oxygen Species , WaterABSTRACT
At present,medicinal plant extract industry in China develops rapidly.As a modern industry,it does not only use the traditional Chinese medicinal materials as raw materials in conception and content,but also use medicinal plant extract as a core,including worldwide medicinal plant extract.China is an important country of extract production in the world.The present status accompanied with the existed questions and induced factors,countermeasure for development,and suggestion for medicinal plant extract industry in China are discussed in this article.It lays the emphasis on present status of industry for medicinal plant extract which involves the production,application,technology,standard and policy of present status influenced on development stage of plant extract enterprise itself in domestic and international.The development countermeasure and suggestion are put forward from seven aspects.