ABSTRACT
OBJECTIVE: To investigate the neuroprotective effects of hydroxysafflor yellow A(HSYA) on the injury of PC12 cells induced by oxygen-glucose deprivation and reperfusion, and the effect on the expression of metrix metalloproteinase-9(MMP-9) in PC12 cells. METHODS: The PC12 cells were exposed to oxygen-glucose deprivation(OGD), and recoveried for different times. The PC12 cell viability and lactate dehydrogenase(LDH) release were seperately detected by MTT assays and LDH detecting kits, the apoptotic cells were double stained by propidumiodid(PI) and Hoechst33342, and the expression of MMP-9 was detected by immunocytochemistry. RESULTS: Compared to the control group, in OGD 4 h-R 2 h group, cell viability significantly decreased to, (P<0.01). Whereas HSYA improved the injuries induced by OGD-R, reflected on the obvious increased cell viability and reduced releasing of LDH (P<0.01) at the concentration of 1 μmol·L-1. And the result stained by PI and Hoechst33342 showed that HSYA also markedly inhibited the apoptosis of PC12 cell induced by OGD-R, which decreased to(19.7±7.5)% in HSYA 1 μmol·L-1 group(P<0.01). Meanwhile, the expression of active MMP-9 induced by OGD-R was decreased after the treatment of HSYA, similar to positive control edaravone. CONCLUSION: It is proved that HSYA protects the injury and inhibits the apoptosis induced by OGD-R in PC12 cells, and the mechanism may be related to the inhibition of active MMP-9 expression.
ABSTRACT
Objective:To study the expression of MMP-9 and eosinophils in nasal polyps and its influence on the prognosis. Method:Study the expression of MMP-9 and EOS on the tissue of nasal polyps of patients who were cured or recurrent and normal nasal mucosa(NM) by immunohistochemisty and HE staining. Result:In the patients of recurrent group (Group B) , the expression of MMP-9 was much stronger than that of good prognosis group (Group A) and NM. The MMP-9 was stronger in the eosinophil nasal polyps than that of non-eosinophil groups. The percentage of EOS in Group A was 16.67% , while 62.50% in the Group B. Conclusion:The stronger expres-sion of MMP-9 on Group B and eosinophil group indicated that MMP-9 might affect the prognosis of nasal polyps through inducing the number of EOS in the mucosa. MMP-9 may be one of the risk factors for the relapse of nasal polyps.
ABSTRACT
Objective:To investigate the effects of sodium phenylbutyrate(NaPB) on the matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW-1.Methods: CGTHW-1 cells were treated with different concentrations of NaPB,then the invasive ability of CGTHW-1 cells was assessed using Transwell assay.The expression of MMP-9 and TIMP-1 was examined by immunocytochemistry staining and RT-PCR in CGTHW-1 cells.Results: After treatment with NaPB(4 mmol/L) for 72 h,CGTHW-1 cells passing the Transwell were significantly reduced[(29.8?1.77) vs(11.00?2.59),P