ABSTRACT
Objective To explore the effects of miR-449a on the proliferation and apoptosis in human neuroblastoma SH-SY5Y cells.Methods Transferred miR-449a mimics SH-SY5Y cells by LipofectamineTM2000 as experimental group.Cell growth was measured by CCK8 assay,the cell cycle and apoptosis were detected by flow cytometry.Relative expression of c-Myc and Bax/Bcl-2 was detected by Western blot.Results Compare to blank and negative control cells,the proliferation of miR-449a mimics SH-SY5Y cells was decreased(P<0.05).Flow cytometry showed that over-expressed miR-449a resulted in an increase of G0/G1 phase and apoptotic cells.The expression of c-Myc showed significant difference (P<0.05),Expression of pro-apoptotic protein Bax was increased,and that of anti-apoptotic protein Bcl-2 was decreased(P<0.05).Conclusions miR-449a over-expression can inhibit cell proliferation by regulating cell cycles and induce apoptosis mainly through regulating Bcl-2 family proteins expression in SH-SY5Y cells.
ABSTRACT
Objective To investigate the expression and biological function of miRNA-449 a in lung cancer . Methods A case-control study was conducted in 58 patients diagnosed with lung cancer ( carcinoma and adeno-carcinoma) and normal tissue closely adjacent to tumor.MiRNA-449a simulation was designed and synthesized, was dissolved into two different concentrations as 10 and 20 mg/mL.The expression of miRNA-449a in lung cancer tissues and matched normal tissues were detected by Real time PCR .The expression of luciferase gene was detected by chemiluminescence technique.MiRNA-449a mimics on cell apoptosis was evaluated by MTT assay . Results The mean tissues expression levels of miRNA-449 a in squamous carcinoma group and adenocarcinoma group were 1.48 ±1.63 and 1.52 ±1.54 respectively, and were significantly lower than in control group (2.74 ± 1.55 ) ( P<0.01 ) .The average intensity of fluorescent protein in 10 mg/mL group and 20 mg/mL group were 2 115 ±168 and 1 352 ±159 respectively , and were significantly lower than that in control group ( 4 975 ±115 ) ( P<0.01 ) .Conclusions MiRNA-449 a was down-regulated expression in lung cancer and induced apoptosis .