ABSTRACT
Aim To establish the model of oxidative damage in brain of Parkinsons disease(PD)rats induced by levodopa(L-DOPA)with microdialysis technique.Methods PD model rats were induced by intracerebral injection of 6-hydroxyl dopamine(6-OHDA)and were perfused in brain with L-DOPA by using microdialysis technique.Salicylic acid can capture hydroxyl radicals in brain,then yield 2,3-dihydroxy benzyl acid(2,3-DHBA)and 2,5-dihydroxy benzyl acid(2,5-DHBA).Extracelluler dopamine(DA)and its metabolites,2,3-DHBA and 2,5-DHBA in striatum of rats were measured by HPLC-ED before and after L-DOPA treatment.Results Both 2,3-DHBA and 2,5-DHBA in model group were significantly higher than those in control group at 6 and 7 time points respectively(P
ABSTRACT
AIM To establish the method of continuous measuring extracellular Acetylcholine and Choline levels in[FQ(10*2。46,X-WZ]-striatum of freely moving awake rats. METHODS The microdialysis and high performance liquid chromatography(HPLC)-post column Immobilized enzyme reactor(IMER)-electrochemical detection(ED) were used to continuously measure extracellular acetylcholine and choline levels in striatum of freely moving awake rats. RESULTS Extracellular acetylcholine and choline levels were higher at the beginning of perfusion, then decreased gradually. The ACh concentration reached a stable level after 240 min while the Ch continuously decreased in the whole perfusion period of 450 min. The detection limit for ACh and Ch, at a S/N ratio of three, was found to be 150 fmol per injection. In the range of 0.125～1.0 ?mol?L -1 , the relation between the amounts and the peak amplitude was linear. CONCLUSION HPLC-IMER-ED is a sensitive and stable method to measure the ACh and Ch. Coupled with microdialysis, it can been successfully used for monitoring the extracellular ACh and Ch levels of freely moving awake rats. -
ABSTRACT
Aim To provide an approach to drug filtration and the research into damage of brain cell caused by senile retrograde affection and oxidative stresses,a model of increased extracellular hydroxyl radical in striatum of rats was established by intracerebral perfusion of 6-hydroxy dopamine(6-OHDA).Method Microdialysis was used to establish the model,hydroxyl radicals captured by salicylic acid yielded 2,3-dihydroxy benzyl acid(2,3-DHBA) and 2,5-dihydroxy benzyl acid(2,5-DHBA) which were measured by HPLC-ED.Results After 75 minuets of the perfusion with 6-OHDA in brain of rats,the levels of 2,3-DHBA and 2,5-DHBA in the model group increased to 6.6 and 3.4 times compered with the control group respectively;and the 2,3-DHBA was still higher than that of the control group in the whole course of observation(P