ABSTRACT
Objective To observe the influence of modified Sijunzi Decoction on the decrease of immune function of exercise-induced exhaustion rats. Methods Thirty SD rats were given adaptive training, and then 6 dead or non-adaptive rats were dropped out. The remaining 24 SD rats were randomly divided into normal group ( N= 8) , model group ( N = 8) , and modified Sijunzi Decoction group ( MSD group, N = 8) . Normal group did not receive any medication and took diet freely. The rats of model group and MSD group were orally given distilled water or modified Sijunzi Decoction 8 g per day respectively after the modeling by exercise-induced exhaustion. After 61 days, the serum was taken from the three groups, and immunoglobulin lgA, lgG, lgM, and complement C3,C4 were detected. Results The contents of immunoglobulins IgA, IgG, IgM and complements C3, C4 in MSD group were higher than those in the model group ( P<0.01) . Conclusion The decrease of immunity occurs in the process of exercise stress, but can be delayed by modified Sijunzi Decoction..
ABSTRACT
Objective To establish burn-induced esophageal lesion model by adding NaOH with different concentrations in rabbits, and investigate the effect of modified sijunzi decoction on the p53 and Bcl-2.Methods After injection with different concentrations of NaOH, esophagus was dissected and observed anatomically.Rabbits were given a gavage of modified Sijunzi decoction daily for 20 days, and then were injected with NaOH.Esophageal epithelium isolated from each group was observed by hematoxylin-eosin staining.p53 and Bcl-2 protein and mRNA expression was measured with western blot and qRT-PCR, respectively.Results The degree of corrosion of esophageal epithelium was positively correlated with the concentration of NaOH.p53 protein and mRNA levels were increased after NaOH challenge; this increase was inhibited by treatment with modified Sijunzi decoction.Additionally, NaOH decreased Bcl-2 protein and mRNA, which was attenuated by modified Sijunzi decoction.Conclusion Modified sijunzi decoction can relieve the esophageal alkali burning in a dose-dependent manner, suggesting that modified Sijunzi decoction may be a useful strategy to treat chemical injuries in esophageal tissue.