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Objective To prepare hydrophobic chitosan from Catharsius molossus and study its hemostatic effect. Methods The chitosan from C. molossus was used as material to carry out hydrophobic modification and characterization by acylation with lauric anhydride. At the same time, the hemostatic effect was studied by in vitro agglutination test and mouse liver wound experiment. Results The prepared hydrophobic chitosan had a hydrophobic group substitution degree of (11.69 ± 0.14)%, which was higher than that of commercially available hydrophobic chitosan. The in vitro agglutination experiment showed that it could coagulate in (3.42 ± 0.39) s when mixed with blood at a concentration of 6.0 mg/g, which was better than chitosan and hydrophobically modified commercial chitosan. The mouse liver wound experiment showed that chitosan from C. molossus could shorten hemostatic time significantly compared with chitosan and hydrophobically modified commercial chitosan. Conclusion Hydrophobically modified chitosan can significantly improve the coagulation performance of chitosan, and the hydrophobic chitosan from C. molossus is excellent, and it is expected to be a new hemostatic material with great potential.
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Objective: The molecularly imprinted osthole on surface-modified quartz sand was prepared and characterized by SEM and FT-IR. The adsorption properties of molecularly imprinted materials were investigated. Methods: The morphology of MIP was prepared using N-(β-aminoethyl)-γ-aminopropylmethylbimethoxy silane (KH-602) modified quartz sand as supporter, osthole as template, methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as crosslinker, axodiisobutyronitrile (AIBN) as initiator, and methanol as porogen, observed by SEM, and the chemical structure of MIP was characterized by FT-IR. Results: The dynamic adsorption experiment showed that the adsorption capacity of molecularly imprinted materials to osthole gradually reached saturation with the increase of time. The maximum adsorption capacity of molecularly imprinted materials for osthole was studied by static adsorption experiment. The selective adsorption experiment was adopted to study the binding properties and molecule recognition characters of molecularly imprinted materials for osthole. Conclusion: The experimental results showed that MIP had specific recognition selectivity, excellent binding affinity and elution property for ractopamine. The MIP on the adsorption ability of osthole was significantly better than xanthotoxin and imperatorin.
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Objective: To introduce a modified transsection and longitudinal suture in epicanthoplasty and summarize its effectiveness.
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Objective To investigate the effect of the proactive percutaneous catheter drainage(PCD)strategy in the treatment of acute pancreatitis(AP)complicated with necrotic infection.Methods A total of 70 patients with AP complicated with necrotic infection and trea-ted by PCD from January 2016 to November 2017 were prospectively enrolled.The patients were randomly divided into the routine group(n=35)and the modified group(n =35)according to the random number table method.The same indication and principle were performed performed during the first time PCD in both of the two groups.However,the subsequent treatment strategy was different between the two groups.The routine group was given a maximum of 1 adjustment of the drainage tube position or another catheterization.If the drainage effect was still poor,the necrosectomy would be taken through laparoscope,nephroscope or laparotomy directly.While the modified group was given frequent and early drain revision and upsizing of drains in case of lack of clinical improvement.The general data,the total number of PCD pro-cedures,the maximal final drain size,the duration between the first two times of PCD,the total drainage duration,the number of patients need for necrosectomy,the length of hospital stay after drainage,the number of patients need for new ICU admission,the number of patients need for readmission,complications and mortality were compared between the 2 groups.Results The total number of PCD procedures and the maxi-mal final drain size of the modified group was significantly higher than that of the routine group(P<0.05 or P<0.01).The duration be-tween the first two times of PCD in the modified group was significantly shorter than of the routine group(P<0.01).The ratio of patients need for necrosectomy and suffered new-onset multiple organ failure significantly decreased in the modified group,and the difference was sta-tistically significant(P<0.05).There was no significant difference between the two groups in total days of drainage,length of hospital stay after drainage,number of patients need for new ICU admission,number of patients need for readmission,complications and mortality.Conclu-sion The proactive PCD strategy of frequent and early drain revision and upsizing of drains for patients with acute infected necrotizing pan -creatitis when lack of clinical improvement can reduce the proportion of recurrent multiple organ failure and the need for necrosectomy,and it does not increase the risk of related complications.
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Objective To study the operation method of modified Lichtenstein herniorrhaphy in patients with femoral hernia.Methods The clinical data of 37 femoral hernia patients having underwent the modified Lichtenstein herniorrhaphy from January 2010 to January 2017 were retrospectively analyzed. Results The average operation time was 50 min, and average hospitalization day was 5.6 d. During the follow-up period,the postoperative pain and foreign body sensation were mild,no recurrence of femoral hernia was found,and no complications such as postoperative infection,testicular atrophy and painful ejaculation occurred. Conclusions Modified Lichtenstein herniorrhaphy for femoral hernia is effective.
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Objective To evaluate the efficacy of modification and care improvement in endotracheal tubes (ETTs) to prevent ventilator associated pneumonia (VAP) in patients receiving mechanical ventilation (MV).Methods PubMed,China National Knowledge Infrastructure (CNKI) were searched,literature information was extracted and classified according to the inclusion criteria,the data were statistically analyzed.Results Totally 46 clinical research articles and 6 laboratory research articles were involved,the data showed that polyurethane (PU) cuff [23.809％ (35/147)],silver-coated ETTs [4.830％ (37/766)],subglottic drainage duct [19.492％ (759/3 894)],discontinuous subglottic drainage[13.603％ (256/1 882)] were associated with a decreased incidence of VAP compared with polyvinyl (PVC) cuff [40.179％ (45/112)],non-silver-coated ETTs [7.537％ (56/743)],conventional ETTs[45.733％ (1 088/2 379)],continuous subglottic drainage [25.000％ (503/2 012)],the differences in the incidence of VAP were statistically significant,respectively,P＜ 0.05.The incidence of VAP in cylindrical PU cuff was 25.000％ (8/32),the tapered PU cuff was 21.875％ (7/32),the difference in the incidence of VAP was not statistically significant.Conclusions The modification of the ETTs and the care improvement can effectively reduce the incidence of VAP.
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Objective: To evaluate and eliminate the potential bias between data obtained from dry and liquid biochemical assays, making data obtained by different assays matchable. Methods: Bias estimation was performed based on document EP9-. A2. Simple data comparison and methodology validation were performed after the experiment methods were modified with the estimated correction factors and interception. All the collected data were analyzed by EXCEL2007 software. Results: The predicted bias of 4 of the 10 compared items exceeded their corresponding acceptable bias. After being adjusted by the coefficient and interception obtained from linear regression analysis, the four bias was improved and was within the acceptable range. The results of simple data comparison further confirmed this comparability. Conclusion: Based on EP9-A2, we have established a protocol to obtain a consistency of data from different biochemical analyzers, which makes it possible that the detection results of the same patient from different detection systems can be used directly. The protocol has been approved by the experts during the medicinal laboratory accreditation of ISO15189.
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OBJECTIVE:To observe clinical efficacy of Modified buzhong yiqi decoction in the treatment of constipation resulted from chemotherapy for colorectal cancer.METHODS:70 colorectal cancer patients with constipation resulted from chemotherapy were randomly divided into traditional Chinese medicine(TCM) treatment group and western medicine control group.TCM treatment group were given Modified buzhong yiqi decoction and western medicine control group were given PEG4000 orally for 14 days.Clinical efficacies of two groups were compared.RESULTS:Clinical efficacy of TCM treatment group was significantly better than in western medicine control group(P
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Objective To evaluate and eliminate the potential bias between data obtained from dry and liquid biochemical assays,making data obtained by different assays matchable.Methods Bias estimation was performed based on document EP9-A2.Simple data comparison and methodology validation were performed after the experiment methods were modified with the estimated correction factors and interception.All the collected data were analyzed by EXCEL2007 software.Results The predicted bias of 4 of the 10 compared items exceeded their corresponding acceptable bias.After being adjusted by the coefficient and interception obtained from linear regression analysis,the four bias was improved and was within the acceptable range.The results of simple data comparison further confirmed this comparability.Conclusion Based on EP9-A2,we have established a protocol to obtain a consistency of data from different biochemical analyzers,which makes it possible that the detection results of the same patient from different detection systems can be used directly.The protocol has been approved by the experts during the medicinal laboratory accreditation of ISO15189.
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Objective To explore the effects of neurotrophin-3 genetically modified Schwann cells(NT-3-SCs) and neural stem cells(NSCs) combinative transplantation to promote the neurons'survival and axonal regeneration of the spinal cord transected rat. Methods After the transected spinal cord injury(SCI) model was established in SD rats,NT-3-SCs or LacZ report gene modified Scwhann cells(LacZ-SCs) were combinative transplanted with NSCs into the transected site.60 days later,fluorogold(FG) was injected into the caudal spinal cord fo the transected site.7days after FG injected,the sacrifice,cryosection and morphology serious studies were performed to observe the FG labeled neurons in the rostral spinal cord(RSC) of transected site,red nuclei(RN) and the sensorimotor cortex(SMC);The survival neurons in the L_1 Clark's nuclei(CN),RN and SMC were couut,and regenerated axons within or near the transected spinal cord observed. Results From more to less,the order of groups of the survival neurons in the CN,RN and SMC was NT-3-SCS & NSCs group,LacZ-SCs & NSCs group,experimental control group.In the NT-3-SCs & NSCs group and LacZ-SCs & NSCs group,there were 5-HT,CGRP and SP positive axons within or near the transected spinal cord.And some FGlabeled cells were found in RSC,RN and SMC. Conclusion Combinative grafting NSCs and NT-3-SCs could promote the neurons'survival and axonal regeneration of the spinal cord injured rat.