ABSTRACT
Morphological examination of blood cells is an important re-examination method of blood cell analysis, and it is also the basis of diagnosis of hematological diseases with cytopathic changes. Artificial intelligence assisted blood cell morphological examinationcan effectively make up for the shortcomings of artificial microscope examination methods, greatly improve the working efficiency of cell morphological examination, solve the problems of manpower shortage and labor intensity to a great extent, obviously improve the repeatability of examination results, facilitate the review of the examination results, and realize remote blood cell morphological examination and diagnosis online. However, at present, the main method of blood cell morphological examination in China is still manual microscope examination. In order to promote and expand the clinical application of artificial intelligence-assisted blood cell morphological examination, the R&D manufacturers and clinical laboratory experts of automatic blood cell morphological analyzer should work together to promote the standardization of automatic blood cell morphological analysis, strengthen its performance evaluation and verification, continuously improve its analytical performance, expand its clinical application scope, and promote the construction of blood cell morphological database in China.
ABSTRACT
Objective To study the laboratory features of Stephanoascus ciferrii isolated from middle ear excretion of the patients with chronic suppurative otitis media for providing the basis of diagnosis for the rare fungus infection.Methods The study was performed for 12 patients who were suffered from chronic suppurative otitis media associated with Stephanoascus ciferrii.The secretions of middle ear were cultured and the macroscopic features of colony and cells of the fungi were observed.The species of the fungi was identified by Vitek 2 Compact system.Results Stephanoascus ciferrii may grow on common media such as blood plate,chocolate plate and sabourauad plate,at 28 to 35 ℃.The form of the colonies displayed cream-colored,rough,hard and wrinkled quality after 1 week incubation.On CHROMagar medium the colonies looked like small mushroom with blue center and white edge.Under microscopy,abundant branched septate mycelium with small ramified chains of oval blastoconidia and the conidia were arranged along the side of hyphae.The fungi strain may assimilate lots of carbohydrates and common amino acid.Vitek 2 Compact automated system should be able to identify Stephanoascus ciferrii.Conclusion Stephanoascus ciferrii is a rare opportunistic pathogenic fungus.In clinical laboratories special attentions should be paid to culture and identification of the fungus from middle ear excretion.