ABSTRACT
Objective To investigate the expression and significance of myeloid cell leukemia-1 (Mcl-1 )gene and protein in Hepatocellular Carcinoma(HCC).Methods The expression of Mcl-1 was detected respectively by Reverse Transcription-Polymerase Chain Reaction (RT-PCR),Western blot and ENVISION immunohistochemistry in 20 HCC specimens,19 liver cirrhosis(LC)specimens,and 12 control ones.Their relationship with clinical and pathological characteristics of HCC was investigated.Results The expression of Mcl-1 mRNA in the control group,LC group and HCC group was 0.52±0.17, 3.46±1.7,3.65±2.93,respectively.The level in HCC and LC group was statistically different compared with the control group,respectively (t=7.925,5.334,P<0.05).The relative expression of Mcl-1 protein in LC group (0.51±0.35)and HCC group (0.75±0.36)were significantly higher than that in the control group (0.21±0.19)(t=5.526,6.355,P<0.05).The positive expression rate of Mcl-1 in HCC group was 55.00% (11/20),significantly higher than that in the con-trol group 33.33% (4/12)(t=7.835,P<0.05).The positive expression of Mcl-1 was related to tumor necrosis and TNM staging (χ2=4.201,P<0.05).Conclusion Mcl-1 gene and protein are differentially expressed in HCC,LC and the control, which may be involved in the occurrence,development and malignant transformation of HCC.
ABSTRACT
Objective. Myeloid cell leukemia-1 (Mcl-1) protein is one of the numbers in bel-2 superfamily, which plays an important role in controlling apoptosis, proliferation, and differentiation of tumor cells. This study was to detect the expression of Mcl-1 protein in different stages of human cervical lesions and to explore further their possible roles in the formation and progression of cervical cancer. Methods: The expression of Mcl-1 protein was detected in 83 paraffin-embedded specimens of the cervical tissues with immunohistochemical SP methods. Its clinical significance was analyzed associating with clinicopathological classification. Results: The positive rate of Mcl-1 protein expression was 4.0% 33.3%, and 74.2% in cervicitis, cervical intraepithelial neoplasia (CIN) and cervical cancer tissue, respectively. The difference was significant among the three groups (P 0.05). Conclusion: Over-expression of Mcl-I may play an important role in the initiation and development of cervical cancer.