ABSTRACT
Objective To investigate the protective effect of short-term moderate exercise on ischemic/reperfused myocardium and its correlation to the activation of protein kinase C(PKC).Methods Fourty Wistar rats were randomly divided into 4 groups(n=10 respectively): control group(CON group),exercise group(EXE group),exercise + PKC inhibitor group(E+C group) and PKC inhibitor group(CHE group).The occurrence of arrhythmia,the recovery of cardiac function,and infarct size were observed by using the Langendorff-ischemia/reperfusion model in isolated rat heart in vitro.Results Recovery rate of LVDP(on the 30th and 60th minute of reperfusion) and RPP(on the 20th,30th and 60 minute of reperfusion) of EXE group were higher than those of CON and E+C groups(P
ABSTRACT
Repeated calcium depletion and repletion of short term duration Ca2+ preconditioning CPC is hypothesized to protect the heart from lethal injury after exposing it to the Ca2+ paradox (Ca2+ PD). Hearts were preconditioned with five cycles of Ca2+ depletion (1 minute) and Ca2+ repletion (5 minutes). These hearts were then subjected to Ca2+ PD,ie,one cycle of Ca2+ depletion (ten minutes) and Ca2+ repletion (ten minutes). Hearts subjected to the Ca2+ PD underwent rapid and severely injury. CPC showed remarkable protection against the Ca2+ PD injury. Protein release from the perfusated heart was significantly reduced in CPC hearts compared with nopreconditioned Ca2+ PD hearts (0. 56 + 0. 10 and 1. 23 + 0. 10 mg/ml respectively ,P