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Objective To investigate the role of LncRNAs-MEG3 in the carcinogenesis and progression of nasopharyngeal carcinoma (NPC) and the possible molecular mechanism. Methods qRT-PCR was used to detect the content of MEG3 and miR-543 in NPC cells. Luciferase reporter method was used to study the relation between MEG3 and miR-543, and the changes of cell proliferation and apoptosis induced by MEG3 or KLF4 were analyzed. Western blot was used to detect the expression of KLF4, Bcl-2 and Bax proteins. Results Compared with the control group, the expression of miR-543 in NPC cell line was significantly increased (P < 0.05), while the expression of MEG3 was decreased (P < 0.05). Luciferase report and Western blot showed that MEG3 could regulate the expression of KLF4 by adsorbing miR-543 to inhibit cell proliferation, promote cell apoptosis and affect the expression levels of Bcl-2 and Bax proteins. Conclusion LncRNA-MEG3 could regulate the expression of KLF4 by adsorbing miR-543 and then plays a role in inhibiting the occurrence and development of NPC. It may be a new biomarker for NPC targeted therapy.
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Objective To investigate the radiosensitizing effects of artemisinin on CNE human nasopharyngeal carcinoma cells in vitro.Methods CNE human nasopharyngeal carcinoma cell line was used in this study.Cell growth kinetics was determined by MTT assay.Effect of the drug on radiosensitivity of CNE cells was analyzed by clonogenic assay.The change of cell cycle was measured by flow cytometry.Results The inhibition of CNE cells growth by artemisinin was increased with concentrations.Artemisinin (1 μmol/L)could enhance the radiosensitizing effects on CNE cell line,and the sensitizing enhancement ratio(SER)was 1.26.Artemisinin abrogated radiation-induced G2/M arrest of the tested CNE cells.Compared with the radiation alone group,the proportion of G2/M phase cells increased in radiation combined with drug group.Conclusions Artemisinin could reduce radiation-induced G2/M arrest and enhance the cytotoxicity of γ-irradiation on the CNE ceils.
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The effect of vascular endothelial growth factor (VEGF) overexpression on matrix metalloproteinase-2 (MMP-2) in nasopharyngeal carcinoma (NPC) cells in vitro and the possible mechanism involved were investigated, and the correlation between the expression of VEGF and MMP-2 in NPC evaluated. The NPC cells were transfected with PAd-trackVEGF165 plasmid. The expression levels of VEGF and MMP-2 mRNA and protein in NPC cells were detected by semi-quantitative RT-PCR and Western blot respectively. It was found that the expression of VEGF and MMP-2 mRNA and protein was significantly increased in NPC cells after transfection of VEGF165. It was concluded that the expression of VEGF was correlated to the in vitro invasion of NPC cells, and the induction of MMP-2 by VEGF was a key process of NPC cell invasion.
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OBJECTIVE:To evaluate the inhibitory actions of 3 traditional Chinese drugs on human nasopharyngeal carcinoma cells CNE-2 in vitro.METHODS:The IC50(50% inhibiting concentration)of 3 traditional Chinese drugs on human nasopharyngeal carcinoma cells CNE-2 in vitro was measured by MTT assay.RESULTS:The inhibitory actions of 3 traditional Chinese drugs on human nasopharyngeal carcinoma cells CNE-2 in vitro were enhanced with the increase of the concentration in a concentration-dependent manner,with formulation Ⅲ showing the most potent inhibitory action on CNE-2 cells in vitro.CONCLUSION:The heat-clearing and detoxicating traditional Chinese drugs could markedly inhibit the proliferation of CNE-2 cells.
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Objective To study the inhibitory effect of berberine on human nasopharyngeal cancer cell line CNE-2 in vivo and in vitro. Methods CNE-2 cell proliferation was measured by MTT assay and cell cycle was analyzed by flow cytometry. Cell morphology was observed with transmission electron microscopy. The cell cycle relative protein was detected by Western blotting. DNA and protein syntheses were assessed by the cellular incorporation of ~ 3 H-TdR and ~ 3 H-Leu, respectively. Anti-tumor activity of berberine in the experimental transplantation tumor CNE-2 was evaluated by relative tumor growth ratio. Results Berberine inhibited CEN-2 cells growth in a time-and dose-dependent manner. MTT Assay showed that the IC_ 50 values of 48 and 72 h were (49.5?5.8) and (13.3?2.0) ?mol/L, respectively. Cell cycle analyses of 50.0 ?mol/L berberine-treated CNE-2 cells by flow cytometry showed the accumulation of cells in the G_2/M phase while 25.0 ?mol/L berberine treatments for 48 h induced apoptosis with the index of (48.9?10.4)%. The inhibition of CNE-2 cell growth by berberine was associated with suppression of cyclin B1, CDK1, and cdc25c proteins. After the treatment of berberine at dose of 30 mg/kg, the median tumor volume was 317.9 mm~3 which was much lower than that in control group (P
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AIM To study the effects of homoharringtonine(HHT) on inhibition of proliferation and induction of apoptosis of nasopharyngeal carcinoma cells CNE 2Z. METHODS The inhibitory rates of the proliferation and IC 50 were detected by MTT method. The apoptosis was analyzed by flow cytometry (FCM), agarose gel electrophoresis and Hoechst 33258/PI fluorescence staining. RESULTS After cells were treated with HHT of different concentrations for 24, 48 and 72 h,respectively,the inhibitory rates of the proliferation rised with concentration and time. The IC 50 values of 24, 48 and 72 h were (0 629?0 039), (0 483?0 011) and (0 389?0 027) mg?L -1 , respectively. The difference among IC 50 values was obvious ( P
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Objective:To study whether caspase-6 was activated during resveratrol(Res)- induced apoptosis of nasopharyngeal carcinoma cells CNE-2Z. Methods:The cleavage of pro-caspase-6 was analyzed by Western-blot. TTie changes of caspase-6 mKNA was detected by semi-quantitative RT-PCR. The changes of caspase-6 activity was determined by colorimetric assay. Results: After CNE-2Z cells were treated with 0 (control), 25, 50, 100, 200 pmol/L Res for 24 h, respectively, the expression of pro-caspase-6 was decreased with concentration increasing. Caspase-6 active fragment P20(20 kD) appeared at 100 fjtmol/L and was increased at 200 (junol/L.The expression of caspase-6 mR-NA was increased in a concentration-dependent manner ( P