ABSTRACT
Objective To study the expression and significance of Neogenin,Hepcidin and serum ferritin(SF)in patients with cancer-related anemia.Methods To test the serum levels of Neogenin,Hepcidin and SF in 107 cases,including 55 cases with cancer-related anemia and 52 cases of non-anemia with tumor by ABC-ELISA.Results ①The serum levels of Neogenin in patients with cancer-related anemia were 0.77 ± 0.45 ng/ml,which were lower than 0.98 ± 0.60 ng/ml in non-anemia group (t=2.003 8,P 0.05).③In patients with cancer,there were positive correlation be-tween Neogenin and Hb (r=0.212 4,t=2.226 4,P 0.05).④There were positive cor-relation between Hepsidin and SF (r=0.486 6,P <0.01).Conclusion There were lower expression of Neogenin,higher ex-pression of Hepcidin in patients with cancer-related anemia is a complex process.The abnormal expression of Neogenin and Hepcidin which were lead to the loss of use of iron are the key factors attribute to anemia in patients with cancer.
ABSTRACT
The underlying effect of different concentrations of neogenin on proliferation,apoptosis and the related proliferative factors in human trophoblasts was explored in order to understand the function of neogenin during placentation.TEV-1 cell line was cultured and the expression of netrin-1was detected by using indirect cellular immunofluorescence.Exponentially growing TEV-1 cells were treated by different concentrations of neogenin(0,1,5,10,50 ng/mL)for 24 h.Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay.TEV-1 cell apoptosis was assessed by flow cytometry(FCM).The expression of netrin-1 mRNA and protein in TEV-1 cells was examined by using real-time PCR and Western blot,respectively.It was found that immunoreactivity for netrin-1 was observed in cytoplasm of the trophoblasts.Immediately after treatment with different concentrations of neogenin for 24 h,the netrin-1 expression began to increase.Real-time PCR revealed that the expression level of netrin-1 mRNA was 37.59±10.25 times higher than control group when TEV-1 cells were exposed to 50 ng/mL neogenin(P<0.01),and the same tendency was seen by using Western blot.MTT results showed that proliferation of TEV-1 cells was independent of neogenin.Meanwhile,apoptosis was significantly increased to(22.15±6.15)% at50 ng/mL neogenin and(6.55±0.25)% without neogenin(P<0.01).It is suggested that neogenin regulates proliferation and apoptosis of TEV-1 cells.And it can enhance the ability of TEV-1 ceils to express netrin-1 in a dose-dependent manner.Neogenin may play an important biological role in the normal human pregnancy and contribute to the physiological pregnancy process.